[1]于洋,刘学政.GPR120通过抑制核因子-κB信号通路对糖尿病大鼠视网膜神经节细胞损伤的保护作用[J].眼科新进展,2021,41(10):905-909.[doi:10.13389/j.cnki.rao.2021.0190]
　YU Yang,LIU Xuezheng.Protective effect of GPR120 on injured retinal ganglion cells in diabetic rats by inhibiting nuclear factor-κB signaling pathway[J].Recent Advances in Ophthalmology,2021,41(10):905-909.[doi:10.13389/j.cnki.rao.2021.0190]
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GPR120通过抑制核因子-κB信号通路对糖尿病大鼠视网膜神经节细胞损伤的保护作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
41卷
期数:
2021年10期
页码:
905-909
栏目:
实验研究
出版日期:
2021-10-05

文章信息/Info

Title:
Protective effect of GPR120 on injured retinal ganglion cells in diabetic rats by inhibiting nuclear factor-κB signaling pathway
作者:
于洋; 刘学政
121001　辽宁省锦州市，锦州医科大学附属第一医院(于洋)；121001　辽宁省锦州市，锦州医科大学解剖学教研室 (刘学政)
Author(s):
YU Yang1; LIU Xuezheng2
1.The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,Liaoning Province,China
2.Department of Anatomy,Jinzhou Medical University,Jinzhou 121001,Liaoning Province,China
关键词:
糖尿病视网膜病变; 视网膜神经节细胞; GPR120; 核因子-κB; 神经损伤
Keywords:
diabetic retinopathy;  retinal ganglion cells;  GPR120;  nuclear factor-κB;  nerve injury
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2021.0190
文献标志码:
A
摘要:
目的　探讨GPR120对糖尿病大鼠视网膜神经节细胞(RGC)的影响及可能机制。方法　健康雄性SD大鼠按55 mg·kg-1腹腔一次性注射链脲佐菌素建立糖尿病大鼠模型。模型诱导成功后，将大鼠随机分成三组：糖尿病组、鱼油预处理组(鱼油1 g·kg-1每天1次灌胃;鱼油的主要成分为omega-3，omega-3为GPR120通路激活剂)、安慰剂组(玉米油1 g·kg-1每天1次灌胃)，每组15只，另取15只正常大鼠作为对照组（对照组和糖尿病组大鼠每天1次灌胃等剂量PBS缓冲液）。12周后，气相色谱法检测各组大鼠视网膜组织中二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）含量，HE染色检测RGC密度，免疫荧光染色检测视网膜GPR120蛋白表达，Western blot检测视网膜GPR120、核因子-κB（NF-κB）、半胱氨酸蛋白酶3（Caspase-3）蛋白相对表达量。结果　与对照组相比，糖尿病组大鼠视网膜组织中DHA、EPA含量均明显减少（均为P<0.01);与糖尿病组相比，鱼油预处理组大鼠视网膜组织中DHA、EPA含量均明显增加（均为P<0.01)。GPR120主要分布于RGC层。对照组大鼠RGC密度为(437.06±4.72) 个·mm-2，GPR120、NF-κB、Caspase-3蛋白相对表达量分别为(35.65±0.89)%、(12.42±0.58)%、(13.76±0.08)%；糖尿病组大鼠RGC密度为 (329.75±3.51) 个·mm-2，GPR120、NF-κB、Caspase-3 蛋白相对表达量分别为(24.14±0.46)%、(38.94±0.45)%、(25.14±0.45)%；鱼油预处理组大鼠RGC密度为(412.44±3.62) 个·mm-2，GPR120、NF-κB、Caspase-3蛋白相对表达量分别为(31.59±0.77)%、(18.11±0.58)%、(20.14±0.61)%。与对照组相比，糖尿病组大鼠视网膜NF-κB、Caspase-3蛋白相对表达量均明显增加，RGC密度、GPR120蛋白相对表达量均明显降低；与糖尿病组相比，鱼油预处理组大鼠视网膜NF-κB、Caspase-3蛋白相对表达量均明显降低，RGC密度、GPR120蛋白相对表达量均明显增加(均为P<0.01)。糖尿病组与安慰剂组相比，各指标差异均无统计学意义(均为P＞0.05)。结论　GPR120激活对糖尿病大鼠RGC损伤具有保护作用，其机制可能与抑制NF-κB通路有关。
Abstract:
Objective　To investigate the effect and possible mechanism of GPR120 on retinal ganglion cells (RGC) in diabetic rats.Methods　Healthy male SD rats were given a one-time intraperitoneal injection of streptozotocin (STZ) at 55 mg·kg-1 to make a diabetic rat model. After the model was successfully induced, the rats were randomly divided into three groups: diabetes group, fish oil pretreatment group (gavage dose was 1 g·kg-1 q.i.d, the main ingredient of fish oil was omega-3, which is the GPR120 pathway activator), placebo group (corn oil 1 g·kg-1, q.i.d), 15 rats in each group, and 15 normal rats were taken as the control group (PBS was given to rats in control group and diabetes group with equal dose, q.i.d). After 12 weeks, the content of DHA and EPA in retinal tissue was analyzed by gas chromatography, RGC density was detected by HE staining, GPR120 protein expression in retina was detected by immunofluorescence staining, and the relative protein expression levels of GPR120, nuclear factor-κB (NF-κB), and Caspase-3 were detected by Western blot.Results　Compared with the control group, the DHA and EPA in the retinal tissue of rats in diabetes group were significantly decreased (both P<0.01); compared with the diabetes group, the DHA and EPA in the retinal tissue of rats in fish oil pretreatment group were significantly increased (both P<0.01). The GPR120 was mainly distributed in RGC layer. The RGC density in the control group was (437.06±4.72) cells·mm-2, and the relative expression levels of GPR120, NF-κB, and Caspase-3 were respectively (35.65±0.89)%, (12.42±0.58)%, and (13.76±0.08)%; the RGC density in the diabetes group was (329.75±3.51) cells·mm-2, the relative expression levels of GPR120, NF-κB, and Caspase-3 were respectively (24.14±0.46)%, (38.94±0.45)%, and (25.14±0.45)%; the RGC density in fish oil pretreatment group was (412.44±3.62) cells·mm-2, the relative expression levels of GPR120, NF-κB and Caspase-3 were respectively (31.59±0.77)%, (18.11±0.58)%, and (20.14±0.61)%. Compared with the control group, the relative expression levels of NF-κB, and Caspase-3 in the diabetes group were significantly increased, the RGC density and the expression of GPR120 were significantly reduced; compared with the diabetes group, the relative expression levels of NF-κB, and Caspase-3 in the fish oil pretreatment group were significantly reduced, RGC density and the expression of GPR120 were significantly increased (all P<0.01). There was no statistical significance in the variation of indexes between the diabetes group and the placebo group (all P>0.05).Conclusion　GPR120 activation has a protective effect on injured RGC in diabetic rats, and its mechanism may be related to the inhibition of NF-κB pathway.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81571383）；辽宁省教育厅课题(编号：L2015324)
更新日期/Last Update: 2021-10-05