[1]刘文强,王玉波,梁汇珉,等.Nogo蛋白受体（NgR）基因沉默对糖尿病大鼠视网膜神经节细胞密度及突触素表达的影响[J].眼科新进展,2017,37(2):106-109.[doi:10.13389/j.cnki.rao.2017.0028]
　LIU Wen-Qiang,WANG Yu-Bo,LIANG Hui-Min,et al.Effects of inhibiting NgR on retinal ganglion cells density and synaptophysin expression of diabetic rats[J].Recent Advances in Ophthalmology,2017,37(2):106-109.[doi:10.13389/j.cnki.rao.2017.0028]
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Nogo蛋白受体（NgR）基因沉默对糖尿病大鼠视网膜神经节细胞密度及突触素表达的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年2期
页码:
106-109
栏目:
实验研究
出版日期:
2017-02-05

文章信息/Info

Title:
Effects of inhibiting NgR on retinal ganglion cells density and synaptophysin expression of diabetic rats
作者:
刘文强; 王玉波; 梁汇珉; 李赵伟; 李铮; 刘学政
121001　辽宁省锦州市，锦州医科大学基础医学院解剖学教研室
Author(s):
LIU Wen-Qiang; WANG Yu-Bo; LIANG Hui-Min; LI Zhao-Wei; LI Zheng; LIU Xue-Zheng
Department of Anatomy,School of Basic Medical Science,Jinzhou Medical University,Jinzhou 121001,Liaoning Province,ChinaResponsible author:LIU Xue-Zheng,E-mail:liuxuezheng168@vip.sina.com;ORCID:0000-0003-2235-4910
关键词:
糖尿病视网膜病变; 视网膜神经节细胞; Nogo蛋白受体; 突触素
Keywords:
diabetic retinopathy; retinal ganglion cell; Nogo receptor; synaptophysin
分类号:
R774
DOI:
10.13389/j.cnki.rao.2017.0028
文献标志码:
A
摘要:
目的　探讨抑制Nogo蛋白受体(nogo receptor,NgR)对糖尿病大鼠视网膜神经节细胞(retinal ganglion cell,RGC)密度及突触素表达的影响。方法　取雄性SD大鼠32只，随机分成正常对照组、糖尿病组、siNgR组及scNgR组，每组8只。后3组腹腔注射链脲佐菌素(50 mg·kg-1)，72 h后尾静脉血糖大于16.7 mmol·L-1的大鼠定为糖尿病模型。siNgR组玻璃体给予腺病毒包装的NgR反义核苷酸序列10 μL，scNgR组玻璃体给予NgR阴性核苷酸序列10 μL，正常对照组及糖尿病组不进行处理。8 周后，HE染色检测RGC密度，免疫荧光技术检测视网膜突触素表达及分布，Western blot检测视网膜NgR及突触素相对表达量。结果　正常对照组、糖尿病组、siNgR组及scNgR组RGC密度分别为(624.33±3.51)个·mm-2、(420.00±2.65) 个·mm-2、(621.67±1.53) 个·mm-2、(416.67±2.52) 个·mm-2。4组间相比，总体差异有统计学意义(F=5985.37，P<0.01)。与正常对照组相比，糖尿病组及scNgR组RGC密度均明显降低(均为P<0.01)，而siNgR组无明显变化(P>0.05)。突触素主要表达于视网膜内网层和外网层，与正常对照组相比，糖尿病组及scNgR组阳性表达减少，荧光强度减弱。而siNgR组无明显变化。正常对照组、糖尿病组、siNgR组及scNgR组NgR蛋白相对表达量分别为：(11.26±0.02)%、(19.38±0.10)%、(11.17±0.02)%、(19.47±0.31)%。4组间比较，总体差异有统计学意义(F=2466.09,P<0.01)；与正常对照组相比，糖尿病组及scNgR组蛋白相对表达量均增加(均为P<0.01)，而siNgR组无明显变化(P>0.05)。突触素蛋白相对表达量分别为：(35.76±0.15)%、(25.47±0.36)%、(35.28±0.12)%、(25.03±0.75)%。4组间比较，总体差异有统计学意义(F=583.70,P<0.01)；与正常对照组相比，糖尿病组及scNgR组蛋白相对表达量均降低(均为P<0.01)，而siNgR组无明显变化(P>0.05)。结论　抑制糖尿病大鼠视网膜NgR表达有助于恢复突触数量，保护受损的RGC。
Abstract:
Objective　To investigate the effects of inhibiting NgR on retinal ganglion cells density and synaptophysin expression of diabetic rat.Methods　Thirty-two SD male rats were randomly divided into normal control group,diabetic group,siNgR group and scNgR group,8 rats in each group.Normal control group was given no any treatment.Diabetes model was induced by intraperitoneal injection of 50 mg·kg-1 streptozotocin in diabetic group,siNgR group and scNgR group,and the blood glucose more than 16.7 mmol·L-1 at 72 hours was set as the successfully model.The rats of siNgR group were intravitreally administrated with anti-NgR nucleotide and the rats of scNgR group intravitreally administrated with negative nucleotide.Eight weeks later,HE staining was conducted to detect density of retinal ganglion cell (RGC),immunofluorescence was used to evaluate the expression and distribution of synaptophysin (a marker of synaptic number).Relative expression of NgR and synaptophysin in retina were analyzed by Western blot.Results　RGC density in normal control group,diabetes group,siNgR group and scNgR group were (624.33±3.51)mm-2,(420.00±2.65)mm-2,(621.67±1.53)mm-2,(416.67±2.52)mm-2,respectively.There was significant difference among four groups (F=5985.37,P<0.01).Compared with normal control group,RGC density in diabetes group and scNgR group were obviously decreased (all P<0.01),but siNgR group had no obviously change (P>0.05).The synaptophysin mainly expressed in the inner and outer network layer.Compared with normal control group,the positive expression of synaptophysin in diabetes group and scNgR group were decreased,but siNgR group had no obviously change.The relative expression of NgR in normal control group,diabetes group,siNgR group and scNgR group were (11.26±0.02)%,(19.38±0.10)%,(11.17±0.02)%,(19.47±0.31)%,respectively.There was significant difference among four groups (F=2466.09,P<0.01).Compared with normal control group,the relative expression of NgR in diabetes group and scNgR group were obviously decreased (all P<0.01),but siNgR group had no obviously change(P>0.05).The relative expression of synaptophysin in normal control group,diabetes group,siNgR group and scNgR group were (35.76±0.15)%,(25.47±0.36)%,(35.28±0.12)%,(25.03±0.75)%,respectively.There was significant difference among four groups (F=583.70,P<0.01).Compared with the normal control group,the expression of synaptophysin in diabetic group and scNgR group were decreased increased (all P<0.01),while there was no significant difference in siNgR group (P>0.05).Conclusion　Inhibiting the expression of NgR in the retina of diabetic rats can help to restore the number of synapses and protect the damaged RGC.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：81571383）
更新日期/Last Update: 2017-03-01