[1]马殿伟,谢学军,张梅,等. 补肾活血剂对高糖和（或）缺氧状态下共同培养视网膜神经节细胞和Müller细胞谷氨酸代谢的影响[J].眼科新进展,2015,35(5):419-423.[doi:10.13389/j.cnki.rao.2015.0114]
　MA Dian-Wei,XIE Xue-Jun,ZHANG Mei,et al. Effects of BuShenSuoXue decoction on glutamate metabolism in retinal ganglion cells and Müller cells cocultured in high glucose concentration or ( and) hypoxic conditions[J].Recent Advances in Ophthalmology,2015,35(5):419-423.[doi:10.13389/j.cnki.rao.2015.0114]
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 补肾活血剂对高糖和（或）缺氧状态下共同培养视网膜神经节细胞和Müller细胞谷氨酸代谢的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年5期
页码:
419-423
栏目:
实验研究
出版日期:
2015-05-05

文章信息/Info

Title:
 Effects of BuShenSuoXue decoction on glutamate metabolism in retinal ganglion cells and Müller cells cocultured in high glucose concentration or ( and) hypoxic conditions
作者:
 马殿伟; 谢学军; 张梅; 万李
 610041　四川省成都市，武警四川总队医院成都分院五官科
Author(s):
 MA Dian-Wei ;  XIE Xue-Jun ; ZHANG Mei; WAN Li
 Department of Ophthalmology and Otorhinolaryngology , Chengdu Hospital of Sichuan PAPF, Chengdu 610041 , Sichuan Province . China
关键词:
 ; 糖尿病视网膜病变; 补肾活血剂; 视网膜Müller细胞; 视网膜神经节细胞
Keywords:
 ; diabetic retinopathy ;  BuShenHuoXue ;  retinal Muller cells ;  retinal ganglion cells
DOI:
10.13389/j.cnki.rao.2015.0114
文献标志码:
A
摘要:
 目的　应用中药血清药理学方法探讨补肾活血中药复方对体外共同培养的视网膜神经节细胞和Ｍüｌｌｅｒ细胞谷氨酸（ｇｌｕｔａｍａｔｅ，Ｇｌｕ）代谢的影响。方法　氨基酸分析仪检测共同培养细胞外液中Ｇｌｕ释放量，［３Ｈ］标记的Ｄ，Ｌ-Ｇｌｕ摄入量判断共同培养细胞的Ｇｌｕ摄取功能测定中药血清干预高糖和（或）缺氧状态下体外共同培养细胞外液中Ｇｌｕ的释放量和摄入量。结果　在４８ｈ时段，缺氧组及高糖缺氧组共同培养细胞的Ｇｌｕ释放量均比正常对照组明显增加（均为Ｐ＜０．０５），而高糖组Ｇｌｕ释放量比正常对照组明显减少（Ｐ＜０．０５）；补肾活血中药血清能明显降低正常条件下（２４ｈ、４８ｈ、７２ｈ）、缺氧及高糖缺氧条件下（４８ｈ、７２ｈ）共同培养细胞的释放量（均为Ｐ＜０．０５）。在２４ｈ、４８ｈ、７２ｈ时段缺氧组及高糖缺氧组共同培养细胞的Ｇｌｕ摄入量均比正常对照组明显降低（均为Ｐ＜０．０５）；高糖组共同培养细胞的Ｇｌｕ摄取功能在２４ｈ和７２ｈ时段均比正常对照组增强（均为Ｐ＜０．０５），但４８ｈ时段Ｇｌｕ摄入比正常对照组降低（Ｐ＜０．０５）；补肾活血中药血清能明显提高正常及缺氧条件下２４ｈ、４８ｈ时段、高糖条件下４８ｈ时段以及高糖和（或）缺氧条件下７２ｈ时段共同培养细胞的Ｇｌｕ摄入量（均为Ｐ＜０．０５）。结论　高糖和（或）缺氧能够引起体外共同培养的视网膜神经节细胞和视网膜Ｍüｌｌｅｒ细胞的Ｇｌｕ代谢障碍，这可能是补肾活血法对高糖和（或）缺氧状态下视网膜神经节细胞的保护作用机制之一。
Abstract:
 Objective To discuss the effects of BuShenSuoXue ( BSHX) decoction on glutamate ( Glu) metabolism in retinal ganglion cells and Miiller cells co-cultured in vitro in high glucose concentration or ( and) hypoxic conditions using serum pharmacology. Methods Serum with BSHX decoction was obtained by the serum pharmacology method of traditional Chinese medicine. Glu intake of the co-cultured cells was determined with intracellular [ 3H ] labeled D . L-Glu concentration by isotopic techniques. The content of Glu in the extracellular fluid was detected with a full-automatic biochemical analyzer. Results The release of Glu of co-cultured cells in the hypoxic group and the combination of high glucose and hypoxic group were increased significantly in comparison to the control group at 48 hours ( all P < 0. 05 ) , and the release of Glu from co-cultured cells in the high glucose group was decreased significantly compared with the control group at 48 hours (P < 0. 05) ; The serum of BSHX decoction could therefore significantly decrease the release of Glu of co-cultured cells in the control group ( at 24 hours ,48 hours and 72 hours) , hypoxic group and high glucose and hypoxic group at 24 hours .48 hours and 72 hours ) ( all P < 0. 05 ) . The Glu intake of co-cultured cells in the hypoxic group, and the high glucose and hypoxic group were significantly reduced compared with the control group at 24 hours .48 hours and 72 hours ( all P <0. 05 ) . the Glu intake of co-cultured cells in the high glucose group strengthened compared with the control group at 24 hours and 72 hours ( all P < 0. 05 ) ,but was lower than the control group at 48 hours (P < 0. 05 ) ;The serum of BSHX decoction could remarkably increase the Glu intake of co-cultured cells in the control group and hypoxic group at 24 hours and 48 hours, the high glucose concentration group at 48 hours and the high glucose and hypoxia group at 72 hours ( all P < 0. 05) . Conclusion The high glucose or( and) hypoxia can cause Glu metabolism dysfunction in the co-cultured retinal ganglion cells and Miiller cells. this may be one of the protection mechanisms of BSHX to the retinal ganglion cells under high glucose concentration or ( and) hypoxic conditions.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：８１０７２４８５）
更新日期/Last Update: 2015-04-27