[1]周琼,肖昂. 雷珠单抗对糖尿病大鼠早期视网膜组织结构的影响[J].眼科新进展,2015,35(9):827-831.[doi:10.13389/j.cnki.rao.2015.0226]
　ZHOU Qiong,XIAO Ang. Effects of ranibizumab on retinal structure at early stage of diabetic rats[J].Recent Advances in Ophthalmology,2015,35(9):827-831.[doi:10.13389/j.cnki.rao.2015.0226]
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 雷珠单抗对糖尿病大鼠早期视网膜组织结构的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年9期
页码:
827-831
栏目:
实验研究
出版日期:
2015-09-05

文章信息/Info

Title:
 Effects of ranibizumab on retinal structure at early stage of diabetic rats
作者:
 周琼; 肖昂
 330006　江西省南昌市，南昌大学第一附属医院眼科
Author(s):
 ZHOU Qiong; XIAO Ang
 Department of Ophthatmology , the First Affiliated Hospital of Nanchang University. Nanchang 330006 , Jiangxi Province , China
关键词:
 ; 糖尿病视网膜病变; 雷珠单抗; 血管内皮生长因子; IV型胶原; 视网膜神经节细胞
Keywords:
 ; diabetic retinopathy ;  ranibizumab ;  vascular endothelial growth factor ;  type lV collagen; retinal ganglion cell
DOI:
10.13389/j.cnki.rao.2015.0226
文献标志码:
A
摘要:
 目的　探讨雷珠单抗对糖尿病大鼠早期视网膜组织结构的影响。方法　４０只ＳＤ大鼠建立糖尿病模型后随机分为４组，每组各１０只，其中Ａ组造模后第２周右眼玻璃体内注射雷珠单抗１μＬ，Ｂ组造模后第４周右眼玻璃体内注射雷珠单抗１μＬ，ａ组造模后第２周右眼玻璃体内注射无菌ＰＢＳ缓冲液１μＬ，ｂ组造模后第４周右眼玻璃体内注射无菌ＰＢＳ缓冲液１μＬ。另１０只正常ＳＤ大鼠作为空白对照组。于第６周和第８周分别取各组大鼠右眼视网膜行苏木精-伊红染色和免疫荧光共聚焦检查。结果　造模后第６周，Ａ组和Ｂ组的神经节细胞数量分别为（２０．７３±０．９３）个和（１９．５３±０．９６）个，与ａ组的（１０．５３±０．８８）个和ｂ组的（１１．５３±０．９６）个相比，差异均有显著统计学意义（均为Ｐ＜０．０１），且Ｂ组Ⅳ型阳性（Ⅳ ＋型）胶原丝条数量为（６．８７±０．９６）条，与ａ组的（１４．７３±１．００）条和ｂ组的（１４．８０±０．９１）条相比，差异均有显著统计学意义（均为Ｐ＜０．０１）。造模后第８周，Ａ组和Ｂ组神经节细胞数量分别为（１９．８０±１．０１）个、（１９．４７±０．９９）个，与ａ组的（６．６７±０．９０）个和ｂ组的（５．８０±０．９４）个相比，差异均有显著统计学意义（均为Ｐ＜０．０１），且Ｂ组Ⅳ ＋型胶原丝条数量为（２．２０±０．７７）条，与ａ组的（１４．３３±０．９８）条和ｂ组的（１５．６７±０．９０）条相比，差异均有显著统计学意义（均为Ｐ＜０．０１）。Ａ组在第６周时的神经节细胞数量与同时间点的Ｂ组有明显差异（Ｐ＜０．０１），而在第８周时差异无统计学意义（Ｐ＞０．０５），且Ａ组在８周观察期内未观察到Ⅳ ＋型胶原丝条。结论　右眼单次玻璃体内注射雷珠单抗１μＬ，可延缓糖尿病大鼠早期视网膜组织结构病变的发生发展，且造模后第２周用药的作用效果优于第４周。
Abstract:
 Objective To investigate the effects of ranibizumab on retinal structure at early stage of diabetic rats. Methods The diabetes models were established in 40 SD rats , and randomly divided into four groups , 10 cases rn each group. The rats in group A were given the right intravitreal injection with I VL ranibizumab in 2 weeks after modeling , group B received intravitreal injection with I VL ranibizumab in 4 weeks after modeling , group a received sham injections with I VL PBS in 2 weeks after modeling , group b received sham injections with I VL PBS in 4 weeks after modeling , while 10 normal rats were assigned as control group. The right rat retinas were obtained for flat preparation at 6 weeks and 8 weeks after modeling. Fluorescence imaging techniques for flat retinal preparations and retinal histopathological observation were used. Results At 6 weeks after modeling,compared with group a( 10. 53 +0. 88) or group b ( 11. 53 + 0. 96) .there were significantly differences of retinal ganglion cells ( RGC) in group A ( 20. 73 + 0. 93 ) and group B ( 19. 53 + 0. 96 ) ( all P < 0. 01 ) , and there were sigruficant differences of IV + collagen strands in group B (6. 87 +0. 96) compared with group a ( 14. 73 + 1. 00) or group b ( 14. 80 + 0. 91 ) ( all P < 0. 01) . At 8 weeks after modeling , RGC number in group A( 19. 80 + 1. 01 ) and group B ( 19. 47 + 0. 99 ) was considerably different from group a( 6. 67 + 0. 90 ) or group b ( 5. 80 + 0. 94 ) ( all P < 0. 01 ) , and it was considerably different of lV + collagen strands in group B( 2. 20 +0. 77 ) compared with group a ( 14. 33 +0. 98 ) or group b ( 15. 67 + 0. 90 ) ( all P < 0. 01 ) . In addition , there was a significant difference in the RGC level between group A and B at 6 weeks after modeling ( P < 0. 01) ,but not at 8 weeks , and lV + collagen strands had not been found in group A during 8 weeks of observation period. Conclrision The right intravitreal injection of I VL ranibizumab can delay the development of early retinopathy of diabetic rats .moreover the effects of drug given at 2 weeks after modeling are better than that at 4 weeks.

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更新日期/Last Update: 2015-08-31