[1]张秀艳,毕爱玲,吴姗姗,等.电针刺激对单眼形觉剥夺弱视大鼠初级视皮层中多巴胺、γ-氨基丁酸及其受体mRNA表达的影响[J].眼科新进展,2020,40(12):1106-1109.[doi:10.13389/j.cnki.rao.2020.0246]
　ZHANG Xiuyan,BI Ailing,WU Shanshan,et al.Effect of electroacupuncture on expression of dopamine, γ-aminobutyric acid and its receptor mRNA in primary visual cortex of monocular deprivation amblyopic rats[J].Recent Advances in Ophthalmology,2020,40(12):1106-1109.[doi:10.13389/j.cnki.rao.2020.0246]
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电针刺激对单眼形觉剥夺弱视大鼠初级视皮层中多巴胺、γ-氨基丁酸及其受体mRNA表达的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年12期
页码:
1106-1109
栏目:
实验研究
出版日期:
2020-12-05

文章信息/Info

Title:
Effect of electroacupuncture on expression of dopamine, γ-aminobutyric acid and its receptor mRNA in primary visual cortex of monocular deprivation amblyopic rats
文章编号:
1106
作者:
张秀艳; 毕爱玲; 吴姗姗; 徐福如; 毕宏生
250002　山东省济南市，山东中医药大学附属眼科医院（张秀艳，徐福如，毕宏生）；250002　山东省济南市，山东省中西医结合眼病防治重点实验室，山东省高校中西医结合眼病防治技术（强化）重点实验室，山东中医药大学眼科研究所（毕爱玲）；610041　四川省成都市，四川大学华西医院（吴姗姗）
Author(s):
ZHANG Xiuyan1; BI Ailing2; WU Shanshan3; XU Furu1; BI Hongsheng1
1.Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine
2.Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases,Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong,Eye Institute of Shandong University of Traditional Chinese Medicine
3.West China Hospital,Sichuan University
关键词:
多巴胺; γ-氨基丁酸; 电针刺激; 初级视皮层; 弱视
Keywords:
dopamine;  gamma-aminobutyric acid;  electroacupuncture;  primary visual cortex;  amblyopia
分类号:
R777.4
DOI:
10.13389/j.cnki.rao.2020.0246
文献标志码:
A
摘要:
目的　探讨电针刺激对单眼形觉剥夺弱视大鼠初级视皮层中多巴胺（dopamine,DA）、γ-氨基丁酸（gamma-aminobutyric acid,GABA）及其受体（D1受体和GABAA受体）mRNA表达的影响。方法　选取36只鼠龄13 d的SD大鼠，随机分为3组：正常对照组、弱视模型组、电针治疗组，每组12只。正常对照组大鼠不做任何处理，弱视模型组、电针治疗组大鼠右眼进行单眼形觉剥夺弱视造模。电针治疗组大鼠在生后30~60 d选取左侧太阳、合谷，右侧太阳、攒竹进行电针刺激干预，百会和右侧合谷仅给予针刺，不通电。每天同一时间段电针刺激干预30 min，频率2 Hz，脉冲长度0.1 s，每周至少干预6 d。采用高效液相色谱-紫外检测器和实时荧光定量PCR检测各组大鼠初级视皮层中DA、GABA含量以及D1受体、GABAA受体 mRNA的表达情况。结果　弱视模型组大鼠初级视皮层中DA、GABA的含量均低于正常对照组，差异均有统计学意义（均为P<0.05）；电针治疗组DA的含量低于正常对照组，差异有统计学意义（P<0.05）；而GABA的含量与正常对照组差异无统计学意义（P>0.05）。电针治疗组大鼠初级视皮层中DA、GABA的含量均高于弱视模型组，差异均有统计学意义（均为P<0.05）。弱视模型组和电针治疗组大鼠初级视皮层中D1受体、GABAA受体 mRNA的表达均低于正常对照组，差异均有统计学意义（均为P<0.05）；电针治疗组GABAA mRNA的表达高于弱视模型组，差异有统计学意义（P<0.05），而D1受体 mRNA的表达与弱视模型组差异无统计学意义（P>0.05）。结论　电针刺激会促进弱视大鼠初级视皮层中DA、GABA及其GABAA受体 mRNA的表达。
Abstract:
Objective　To explore the effect of electroacupuncture (EA) on the mRNA expression of dopamine (DA), gamma-aminobutyric acid (GABA) and their receptor( D1, GABAA) mRNA in the primary visual cortex of rats with monocular deprived (MD) amblyopia.Methods　Thirty-six SD rats aged 13 days were randomly divided into 3 groups: normal control group, amblyopia model group and amblyopia + EA group, with 12 rats in each group. The rats in the normal control group did not do any treatment. The right eyes of the rats in the amblyopia model group and the amblyopia + EA group were modeled with monocular form deprivation amblyopia. Rats in the amblyopia + EA group were given electroacupuncture stimulation from 30 to 60 days after birth. The stimulating acupoints were left Taiyang, Hegu, right Taiyang, Zuanzhu, Baihui and right Hegu only given acupuncture without electricity. At the same time every day, electroacupuncture was intervened for 30 min with a frequency of 2 Hz and a pulse length of 0.1 s for at least 6 days per week. The expression of DA, GABA and their receptors’ mRNA in the primary visual cortex were detected by high-performance liquid chromatography (HPLC) and quantitative polymerase chain reaction (Q-PCR) techniques.Results　The contents of DA and GABA in the amblyopia model group were significantly lower than those in the normal control group, and the differences were both statistically significant (both P＜0.05); the content of DA in the amblyopia+EA group was significantly lower than that in the normal control group (P＜0.05), whereas there was no significant difference in content of GABA when compared with the normal control group (P＞0.05). The contents of DA and GABA in the amblyopia+EA group were significantly higher than those in the amblyopia model group, and the differences were both statistically significant (all P＜0.05). The expression of D1 and GABAA mRNA in the amblyopia model group and the amblyopia + EA group were significantly lower than those in the normal control group, and the differences were both statistically significant (all P＜0.05). The expression of GABAA mRNA in the amblyopia + EA group was significantly higher than that in the amblyopia model group (P＜0.05), while there was no significant difference in the expression level of D1 mRNA (P＞0.05).Conclusion　Electroacupuncture intervention may promote the expression of DA, GABA and receptor GABAA mRNA in the primary visual cortex of amblyopic rats.

参考文献/References:

［1］　GORE C,WU C.Medical therapies of amblyopia: translational research to expand our treatment armamentarium［J］.Semin Ophthalmol,2016,31(1-2):155-158.
［2］　FU J,LI S M,LI S Y,LI J L,LI H,ZHU B D,et al.Prevalence,causes and associations of amblyopia in year 1 students in Central China: The Anyang childhood eye study (ACES)［J］.Graefes Arch Clin Exp Ophthalmol,2014,252(1):137-143.
［3］　PI L H,CHEN L,LIU Q,KE N,FANG J,ZHANG S,et al.Prevalence of eye diseases and causes of visual impairment in school-aged children in Western China［J］.J Epidemiol,2012,22(1):37-44.
［4］　WU J F,BI H S,WANG S M,HU Y Y,WU H,SUN W,et al.Refractive error,visual acuity and causes of vision loss in children in Shandong,China.The Shandong Children Eye Study［J］.PLoS One,2013,8(12): e82763.
［5］　FAGHIHI M,HASHEMI H,NABOVATI P,SAATCHI M,YEKTA A,RAFATI S,et al.The prevalence of amblyopia and its determinants in a population-based study［J］.Strabismus,2017,25(4):176-183.
［6］　MUI A M,YANG V,AUNG M H,FU J,ADEKUNLE A N,PRALL B C,et al.Daily visual stimulation in the critical period enhances multiple aspects of vision through BDNF-mediated pathways in the mouse retina［J］.PLoS One,2018,13(2): e0192435.
［7］　SALE S,BERARDI N,SPOLIDORO M,BARONCELLI L,MAFFEI L.GABAergic inhibition in visual cortical plasticity［J］.Front Cell Neurosci,2010,4(10):10.
［8］　ZHU T T,CHEN C,YAN X K.Regulation of acupuncture on NMDAR1 mRNA expression in visual cortex of monocularly-deprived rats［J］.J Acupun Tuina Sci,2017,15(1):1-7.
［9］　PAXINOS G,WATSON C.The rat brain in stereotaxic coordinates［J］.J Neurosci Methods,1986,3(2):6.
［10］　PEREIRA C M,FREIRE M A M,SANTOS J R,GUIMARES J S,DIAS-FLORENCIO G,SANTOS S,et al.Non-visual exploration of novel objects increases the levels of plasticity factors in the rat primary visual cortex［J］.Peer J,2018,6:e5678.
［11］　ZHANG H M,MU L W,WANG D D,XIA D D,SALMON A,LIU Q L,et al.Uncovering a critical period of synaptic imbalance during postnatal development of the rat’s visual cortex: role of brain-derived neurotrophic factor［J］.J Physiol,2018,596(18):4511-4536.
［12］　朱田田，邢家铭，严兴科.针刺对单眼剥夺大鼠视皮层突触结构可塑性的调节机制研究［J］.中华中医药杂志，2017，32(5)：2319-2322.
ZHU T T,XING J M,YAN X K.Regulatory mechanism of acupuncture on synaptic plasticity in visual cortex of monocular deprivation rats ［J］.Chin J Tradit Chin Med Pharm,2017,32(5):2319-2322.
［13］　WANG L,FONTANINI A,MAFFEI A.Experience-dependent switch in sign and mechanisms for plasticity in layer 4 of primary visual cortex［J］.J Neurosci,2012,32(31):10562-10573.
［14］　LI R,LIANG T,CHEN Z,ZHANG S,LIN X,HUANG R.L-dopa methyl ester attenuates amblyopia-induced neuronal injury in visual cortex of amblyopic cat［J］.Gene,2013,527(1):115-122.
［15］　RAZEGHINEJAD M R,NOWROOZZADEH M H,EGHBAL M H.Levodopa and other pharmacologic interventions in ischemic and traumatic optic neuropathies and amblyopia［J］.Clin Neuropharmacol,2016,39(1):40-48.
［16］　敖明昕.视觉皮质可塑性及双眼视觉与弱视治疗新理念［J］.中华实验眼科杂志，2017，35(6)：561-566.
AO M X.Treatment of amblyopia emerging from plasticity of the visual cortex and binocular vision［J］.Chin J Exp Ophthalmol,2017,35(6):561-566.
［17］　LEVENTHAL A G,WAN Y,PU M,ZHOU Y,MA Y.GABA and its agonists improved visual cortical function in senescent monkeys［J］.Science,2003,300(5620):812-815.
［18］　邵立功，章应华，张东杲.弱视猫外侧膝状体γ-氨基丁酸能神经元变化的免疫细胞化学研究［J］.中华眼科杂志，1994，30(6)：437-440.
SHAO L G,ZHANG Y H, ZHANG D G.Expression of mGluR1 at primary visual cortex of monocular deprived amblyopia rats and the observation of neuronic ultrastructure［J］.Chin J Ophthalmol,1994,30(6):437-440.
［19］　KEIL W,SCHMIDT K F,LOWEL S,KASCHUBE M.Reorganization of columnar architecture in the growing visual cortex［J］.Proc Natl Acad Sci USA,2010,107(27):12293-12298.
［20］　YAO J P,YIN Z Q,WANG S J.Proteins associated with the termination of ocular dominance column plasticity in long evans rats［J］.Yan Ke Xue Bao,2007,23(3):143-152.
［21］　ZHAO J,LAM D S,CHEN L J,WANG Y,ZHENG C,LIN Q,et al.Ritch R randomized controlled trial of patching vs acupuncture for anisometropic amblyopia in children aged 7 to 12 years［J］.Arch Ophthalmol,2010,128(12):1510-1517.
［22］　KIORES L.The puzzle of visual development: behavior and neural limits［J］.J Neurosci,2016,36(45):11384-11393.
［23］　LAURA R,MATTEO C.Reorganization of visual callosal connections following alterations of retinal input and brain damage［J］.Front Syst Neurosci,2016,10:86.
［24］　曹朝霞，刘安国，朱田田，李小娟，魏玉婷，严兴科.针刺治疗弱视的分子生物学机制研究进展［J］.针刺研究,2018,43(3)：189-193.
CAO Z X,LIU A G,ZHU T T,LI X J,WEI Y T,YAN X K.Molecular biology of the treatment of amblyopia with acupuncture: a review of recent research［J］.Acupuncture Res,2018,43(3):189-193.

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备注/Memo

备注/Memo:
国家重点研发计划项目（编号：2019YFC1710200、2019YFC1710204）；国家自然科学基金资助（编号：81173440、31300907）；山东省自然科学基金资助（编号：BS2014YY041、ZR2013HQ028）
更新日期/Last Update: 2020-12-05