[1]曹长顺,杜益茗,柯宗文,等.小胶质细胞介导的炎症反应在衣霉素所致的小鼠视网膜损伤中的作用[J].眼科新进展,2020,40(12):1110-1114.[doi:10.13389/j.cnki.rao.2020.0247]
　CAO Changshun,DU Yiming,KE Zongwen,et al.Role of microglia-mediated inflammatory response in the retinal injury induced by tunicamycin in mice[J].Recent Advances in Ophthalmology,2020,40(12):1110-1114.[doi:10.13389/j.cnki.rao.2020.0247]
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小胶质细胞介导的炎症反应在衣霉素所致的小鼠视网膜损伤中的作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年12期
页码:
1110-1114
栏目:
实验研究
出版日期:
2020-12-05

文章信息/Info

Title:
Role of microglia-mediated inflammatory response in the retinal injury induced by tunicamycin in mice
文章编号:
1110
作者:
曹长顺; 杜益茗; 柯宗文; 张洪英; 于利; 刘华; 姜双; 车慧欣; 骆玮
121000　辽宁省锦州市，锦州医科大学（曹长顺，杜益茗，柯宗文，张洪英，于利，刘华）；121000　辽宁省锦州市，锦州医科大学附属第三医院眼科（姜双，车慧欣，骆玮）
Author(s):
CAO Changshun1; DU Yiming1; KE Zongwen1; ZHANG Hongying1; YU Li1; LIU Hua1; JIANG Shuang2; CHE Huixin2; LUO Wei2
1.Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China
2.Department of Ophthalmology,the Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China
关键词:
衣霉素; 小胶质细胞; 视网膜损伤; 炎症因子
Keywords:
tunicamycin;  microglia;  retinal injury;  inflammatory factor
分类号:
R774
DOI:
10.13389/j.cnki.rao.2020.0247
文献标志码:
A
摘要:
目的　探讨小胶质细胞介导的炎症反应在衣霉素所致的小鼠视网膜损伤中的作用。方法　取50只C57/BL6J小鼠,随机分为5组，每组10只；DMSO组给予小鼠玻璃体内注射DMSO溶液1 μL；衣霉素低剂量组给予小鼠玻璃体内注射50 mg?L-1衣霉素1 μL,衣霉素中剂量组注射100 mg?L-1衣霉素1 μL,衣霉素高剂量组注射150 mg?L-1衣霉素1 μL；对照组给予相同条件下喂养。采取小鼠玻璃体内注药建模后，分别进行OCT检查及HE染色观察小鼠视网膜组织形态改变；进行闪光视网膜电图检测评估视网膜视功能；免疫荧光染色及Western blot检测视网膜中Iba1及IL-6的表达情况。结果　与对照组相比，衣霉素低剂量组视网膜各层结构紊乱，视网膜外层呈波浪状改变，并出现细胞核排列紊乱；衣霉素中剂量组及高剂量组视网膜各层结构紊乱更加明显，并出现神经上皮与色素上皮的浅层脱离，IS/OS层及外丛状层消失。造模后第7天，闪光视网膜电图结果显示:对照组与DMSO组a波及b波振幅无差异；与对照组相比，衣霉素低剂量组、中剂量组及高剂量组a波、b波振幅下降，且呈剂量依赖性，其中a波振幅下降更为明显，差异均有统计学意义（均为P<0.05）。与对照组相比，衣霉素低剂量组、中剂量组及高剂量组视网膜内核层及外核层中Iba1表达量明显增多，且呈剂量依赖性（均为P<0.05）；IL-6视网膜中呈现与Iba1相似的表达。结论　小胶质细胞参与衣霉素所致的小鼠视网膜损伤过程，并产生相关炎症因子引起视网膜功能及结构改变。
Abstract:
Objective　To investigate the role of microglial-mediated inflammatory response in retinal damage caused by tunicamycin in mice.Methods　Fifty C57/BL6J mice were randomly divided into 5 groups, each with 10 mice: the DMSO group, in which mice were given intravitreal injection of DMSO solution 1 μL; the low-dose group was given intravitreal injection of 50 mg?L-1 tunicamycin 1 μL; the middle-dose group was injected with 100 mg?L-1 tunicamycin 1 μL, and the high-dose group was injected with 150 mg?L-1 tunicamycin 1 μL; and the control group was fed under the same conditions. After a model of intravitreal injection of mice was established，OCT and HE staining were performed to observe the morphological changes of mouse retina; flash electroretinogram was performed to evaluate retinal visual function; immunofluorescence staining and Western blot were performed to detect the expression of Iba1 and IL-6.Results　Compared with the control group, the low-dose tunicamycin group had a disorder of the various layers of the retina, the outer layer of the retina was wavy, and the nucleus was disturbed; the middle-dose and high-dose groups of tunicamycin had more obvious structural disorders of the retina, and the superficial detachment of the neuroepithelium and the pigment epithelium occurred, and the outer plexiform layer of the IS/OS layer disappeared. On the 7th day after modeling, the flash electroretinogram results showed that there was no difference in the amplitude of a wave and b wave between the control group and the DMSO group; compared with the control group, the amplitudes of a wave and b wave in the low-dose group, medium-dose group and high-dose group decreased in a dose-dependent manner, and the amplitude of the a wave decreased more obviously, and the differences were statistically significant (all P<0.05). Compared with the control group, the expression of Iba1 in the inner and outer nuclear layers of the retina in the low-dose, middle-dose, and high-dose groups of tunicamycin was significantly increased in a dose-dependent manner (all P<0.05). The expression manner of IL-6 was similar to Iba1’s expression.Conclusion　Microglia are involved in the process of mouse retinal damage caused by tunicamycin, resulting in producing related inflammatory factors to cause changes in retinal function and structure.

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备注/Memo

备注/Memo:
辽宁省自然科学基金(编号：81641057、2019-BS-101)；辽宁医学院校长基金-奥鸿博泽基金（编号：XZJJ20140125）
更新日期/Last Update: 2020-12-05