[1]董志章,李娟,孙雪荣,等.强光照致TgAPPswePS1转基因鼠视网膜功能障碍和结构损伤[J].眼科新进展,2017,37(4):317-320.[doi:10.13389/j.cnki.rao.2017.0080]
　DONG Zhi-Zhang,LI Juan,SUN Xue-Rong,et al.Light exposure resulted in APPswe/PS1 transgenic mice retinal structural and functional disorder[J].Recent Advances in Ophthalmology,2017,37(4):317-320.[doi:10.13389/j.cnki.rao.2017.0080]
点击复制

强光照致TgAPPswePS1转基因鼠视网膜功能障碍和结构损伤/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年4期
页码:
317-320
栏目:
实验研究
出版日期:
2017-04-05

文章信息/Info

Title:
Light exposure resulted in APPswe/PS1 transgenic mice retinal structural and functional disorder
作者:
董志章; 李娟; 孙雪荣; 葛坚
325200　浙江省温州市，温州医科大学附属第二医院&育英儿童医院眼科（董志章）；710004　陕西省西安市，西安市第四医院眼科（李娟）；523000　广东省湛江市，广东医科大学衰老研究所东莞校区（孙雪荣）；510060　广东省广州市，中山大学中山眼科中心（葛坚）
Author(s):
DONG Zhi-Zhang; LI Juan; SUN Xue-Rong; GE Jian
2nd Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University(DONG Zhi-Zhang),Wenzhou 325200,Zhejiang Province,China;Department of Ophthalmology,the Fourth Hospital of Xi’an(LI Juan),Xi’an 710004,Shaanxi Province,China;The Institute of Aging,Guangdong Medical University,Zhanjiang 523000,Guangdong Province,China;State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University(GE Jian),Guangzhou 510060,Guangdong Province,China
关键词:
光损伤; 视网膜电生理; 转基因鼠; 阿尔茨海默病
Keywords:
light insult; electroretinography; transgenic mouse; Alzheimer’s disease
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2017.0080
文献标志码:
A
摘要:
目的　初步研究在光照损伤作用下TgAPPswePS1转基因鼠视网膜结构和功能的改变情况。方法　给予6月龄阿尔茨海默病转基因鼠（TgAPPswePS1鼠）10 000 lux光照处理，每天光照12 h，连续光照6个月，光照处理为实验组，设鼠龄匹配的转基因鼠为对照组，在光照6个月后（即小鼠12个月时）对实验组与对照组小鼠进行视网膜电图检测，取材并进行切片，行HE染色并观察视网膜各层结构改变，予以视网膜厚度测量并进行统计学分析。结果　对照组Rod反应a、b波波幅分别为（18.33±3.53）μV、（107.58±14.72）μV，Max反应a、b波波幅分别为（64.80±7.57）μV、（178.76±14.47）μV；实验组光照6个月后小鼠视网膜电图Rod反应a、b波波幅分别为（17.92±4.89）μV、（21.83±5.51）μV，Max反应的a、b波波幅分别为（18.23±4.44）μV、（24.50±4.49）μV；与对照组比较，Rod反应的b波和Max反应的a、b波的波幅下降明显，差异均有统计学意义（均为P<0.05）；在Rod和Max反应里a波和b波的潜伏期改变不明显。与对照组比较，经过光照处理后，实验组小鼠视网膜结构发生明显改变，以外核层与感光细胞外节明显，视网膜厚度明显变薄，为（102.34±9.38）μm，与对照组（181.32±13.47）μm比较差异有统计学意义（P=0.017）。结论　高强度光照可以造成TgAPPswePS1鼠视网膜结构损伤和功能障碍。
Abstract:
Objective　To research the functional and structural change in TgAPPswePS1 transgenic mice after intensive light exposure insult.Methods　APPswe/PS1 transgenic mice at the age of 6 months old were grouped for experiments,the transgenic mice were replaced by light insult device for 6 months,while the control mice were kept in normal conditions.After 6 months light exposure,the eyes of control and experimental mice were examined with electroretinography (ERG).The retinal morphology change was investigated with H&E staining.All of the results were quantified and statistically analyzed.Results　In the control group,the amplitudes of a and b wave in the rod response were (18.33±3.53)μV and （107.58±14.72）μV，while （64.80±7.57）μV and （178.76±14.47）μV for the amplitudes of a and b wave in the maximum response;After treated by 6 months of intensive light exposure,in experimental group mice,the amplitudes of a and b wave in the rod response were （17.92±4.89）μV and (21.83±5.51)μV;While in the maximum response a striking decrease was detected with a wave (18.23±4.44)μV and b wave (24.50±4.49)μV,by compared with control group,the difference were statistical significant (all P<0.05).Histopathological analysis found significant loss of outer nuclear layer,photoreceptor out segment,whereas controls remained little change in the retina.And the retinal thickness decreased significantly from (181.32±13.47)μm in control group to (102.34±9.38)μm after light insults in experimental group,the difference was statistical significant (P=0.017).Conclusion　Intensive light exposure can cause the retinal structural and functional disorder in the APPswe/PS1 transgenic mouse.

参考文献/References:

［1］　BLENNOW K，DE LEON MJ，ZETTERBERG H.Alzheimer’s disease［J］.Lancet，2006，368（9533）：387-403.
［2］　JEN LS，HART AJ，JEN A，RELVAS JB，GENTLEMAN SM.Alzheimer’s peptide kills cells of retina in vivo［J］.Nature，1998，392（6672）：140-141.
［3］　NING A，CUI J，TO E，ASHE KH，MATSUBARA J.Amyloid-beta deposits lead to retinal degeneration in a mouse model of Alzheimer disease［J］.Invest Ophthalmol Vis Sci，2008，49（11）：5136-5143.
［4］　PEREZ SE，LUMAYAG S，KOVACS B，MUFSON EJ，XU S.Beta-amyloid deposition and functional impairment in the retina of the APPswe/PS1DeltaE9 transgenic mouse model of Alzheimer’s disease［J］.Invest Ophthalmol Vis Sci，2009，50（2）：793-800.
［5］　董志章，李娟，孙雪荣，葛坚.高强度光照损伤致TgAPPswePS1转基因鼠脉络膜新生血管形成［J］.眼科新进展，2017，37（2）：101-105.
DONG ZZ，LI J，SUN XR，GE J.Intensive light exposure induce choroidal neovascularization in TgAPPswe/PS1 transgenic mice［J］.Rec Adv Ophthalmol，2017，37（2）：101-105.
［6］　SHIMAZAWA M，INOKUCHI Y，OKUNO T，NAKAJIMA Y，SAKAGUCHI G.Reduced retinal function in amyloid precursor protein-over-expressing transgenic mice via attenuating glutamate-N-methyl-d-aspartate receptor signaling［J］.J Neurochem，2008，107（1）：279-290.
［7］　ORGANISCIAK DT，VAUGHAN DK.Retinal light damage：mechanisms and protection［J］.Prog Retin Eye Res，2010，29（2）：113-134.
［8］　CRUICKSHANKS KJ，KLEIN R，KLEIN BE，NONDAHL DM.Sunlight and the 5-year incidence of early age-related maculopathy：the beaver dam eye study［J］.Arch Ophthalmol，2001，119（2）：246-250.
［9］　LAVAIL MM，GORRIN GM，REPACI MA，THOMAS LA，GINSBERG HM.Genetic regulation of light damage to photoreceptors［J］.Invest Ophthalmol Vis Sci，1987，28（7）：1043-1048.
［10］　CAO W，TOMBRAN-TINK J，ELIAS R，SEZATE S，MRAZEK D.In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor［J］.Invest Ophthalmol Vis Sci，2001，42（7）：1646-1652.
［11］　CASSON RJ，CHIDLOW G，WOOD JP，VIDAL-SANZ M，OSBORNE NN.The effect of retinal ganglion cell injury on light-induced photoreceptor degeneration［J］.Invest Ophthalmol Vis Sci，2004，45（2）：685-693.
［12］　ALBERT DM，NEEKHRA A，WANG S，DARJATMOKO SR，SORENSON CM.Development of choroidal neovascularization in rats with advanced intense cyclic light-induced retinal degeneration［J］.Arch Ophthalmol，2010，128（2）：212-222.
［13］　LIU B，RASOOL S，YANG Z，GLABE CG，SCHREIBER SS.Amyloid-peptide vaccinations reduce beta-amyloid plaques but exacerbate vascular deposition and inflammation in the retina of Alzheimer’s transgenic mice［J］.Am J Pathol，2009，175（5）：2099-2110.
［14］　WENZEL A，GRIMM C，SAMARDZIJA M，REME CE.Molecular mechanisms of light-induced photoreceptor apoptosis and neuroprotection for retinal degeneration［J］.Prog Retin Eye Res，2005，24（2）：275-306.
［15］　刘章，周琼.阿尔茨海默病、视神经及特殊蛋白三者关系的研究进展［J］.眼科新进展，2012，32（12）：1194-1196.
LIU Z，ZHOU Q.Recent advances in the relationship among Alzheimer’s disease，optic nerve and especial proteins［J］.Rec Adv Ophthalmol，2012，32（12）：1194-1196.

相似文献/References:

[1]何婷 李根林.视网膜色素上皮层黑色素颗粒对视网膜光损伤保护作用的研究进展[J].眼科新进展,2012,32(12):000.
[2]毕伍牧 孙康. 视网膜光损伤及蓝光滤过型IOL的利弊[J].眼科新进展,2014,34(3):289.
[3]王颖立,牛膺筠.视网膜光损伤中TIMP-1、ERK-1的表达及促红细胞生成素对其影响[J].眼科新进展,2014,34(10):911.[doi:10.13389/j.cnki.rao.2014.0252]
[4]田润,梅妍,郭星,等. 岩茶提取物对大鼠视网膜光损伤的保护作用[J].眼科新进展,2015,35(8):718.[doi:10.13389/j.cnki.rao.2015.0196]
　TIAN Run,MEl Yan,GUO Xing,et al. Protective effects of rock tea extract on light-induced retinal damage in rats[J].Recent Advances in Ophthalmology,2015,35(4):718.[doi:10.13389/j.cnki.rao.2015.0196]
[5]彭素芬,韩泉洪,李丽华,等.LED光源对人视网膜色素上皮细胞分泌单核细胞趋化因子-1和白细胞介素-8的影响[J].眼科新进展,2016,36(3):201.[doi:10.13389/j.cnki.rao.2016.0055]
　PENG Su-Fen,HAN Quan-Hong,LI Li-Hua,et al.Effects of light emitting diode on expression of MCP-1 and IL-8 in cultured human retinal pigment epithelial cells[J].Recent Advances in Ophthalmology,2016,36(4):201.[doi:10.13389/j.cnki.rao.2016.0055]
[6]刘丽芳,黄楚开,林洪杰,等.激光光凝法诱导大鼠高眼压模型的有效性评估[J].眼科新进展,2016,36(3):206.[doi:10.13389/j.cnki.rao.2016.0056]
　LIU Li-Fang,HUANG Chu-Kai,LIN Hong-Jie,et al.Efficiency evaluation on laser-induced hypertensive rat model[J].Recent Advances in Ophthalmology,2016,36(4):206.[doi:10.13389/j.cnki.rao.2016.0056]
[7]董淑倩,刘双珍,李秋明.光预适应对小鼠视网膜光感受器细胞光损伤的保护作用[J].眼科新进展,2018,38(5):412.[doi:10.13389/j.cnki.rao.2018.0096]
　DONG Shu-Qian,LIU Shuang-Zhen,LI Qiu-Ming.Protective effects of light preconditioning on retinal photoreceptor cells aganist light damage[J].Recent Advances in Ophthalmology,2018,38(4):412.[doi:10.13389/j.cnki.rao.2018.0096]
[8]王雪纯,张伟,郭雅图,等.熟地黄、枸杞子及其混合提取物对小鼠视网膜光损伤的保护作用[J].眼科新进展,2021,41(1):012.[doi:10.13389/j.cnki.rao.2021.0003]
　WANG Xuechun,ZHANG Wei,GUO Yatu,et al.Protective effects of rehmannia glutinosa, wolfberry and the mixed extracts on light-induced retinal injury of mice[J].Recent Advances in Ophthalmology,2021,41(4):012.[doi:10.13389/j.cnki.rao.2021.0003]
[9]巩一博,郭雅图,张伟.菊花提取液对小鼠视网膜光损伤的保护作用[J].眼科新进展,2021,41(2):110.[doi:10.13389/j.cnki.rao.2021.0023]
　GONG Yibo,GUO Yatu,ZHANG Wei.Protective effect of chrysanthemum extract against retinal light damage in mice[J].Recent Advances in Ophthalmology,2021,41(4):110.[doi:10.13389/j.cnki.rao.2021.0023]
[10]李娟,陈雨婷,师若迪,等.miRNA-21-5p对光诱导的人视网膜色素上皮细胞氧化应激损伤的影响[J].眼科新进展,2023,43(8):594.[doi:10.13389/j.cnki.rao.2023.0120]
　LI Juan,CHEN Yuting,SHI Ruodi,et al.Effect of micro ribonucleic acid-21-5p on light-induced oxidative stress injury in human retinal pigment epithelial cells[J].Recent Advances in Ophthalmology,2023,43(4):594.[doi:10.13389/j.cnki.rao.2023.0120]

备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81400424）；陕西省科学技术研究发展计划项目（编号：2014K11-03-07-04）；温州市科技计划项目（编号：Y20140140）
更新日期/Last Update: 2017-04-26