[1]田润,梅妍,郭星,等. 岩茶提取物对大鼠视网膜光损伤的保护作用[J].眼科新进展,2015,35(8):718-720.[doi:10.13389/j.cnki.rao.2015.0196]
　TIAN Run,MEl Yan,GUO Xing,et al. Protective effects of rock tea extract on light-induced retinal damage in rats[J].Recent Advances in Ophthalmology,2015,35(8):718-720.[doi:10.13389/j.cnki.rao.2015.0196]
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 岩茶提取物对大鼠视网膜光损伤的保护作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年8期
页码:
718-720
栏目:
实验研究
出版日期:
2015-08-05

文章信息/Info

Title:
 Protective effects of rock tea extract on light-induced retinal damage in rats
作者:
 田润; 梅妍; 郭星; 陈臻; 方林; 李晓芹
 650032　云南省昆明市，云南省第一人民医院（昆明理工大学附属昆华医院）眼科
Author(s):
 TIAN Run ; MEl Yan; GUO Xing; CHEN Zhen ;  FANG Lin ;  LI Xiao-Qin
 Department of Ophthalmology , the First People’s Hospital of Yunnan Province ( Kunhua Hospital Affiliated to Kunming University of Science and Technology ), Kunming 650032 , Yunnan Province. China
关键词:
 ; 岩茶提取物; 视网膜; 光损伤; 抗氧化; 大鼠
Keywords:
 ; rock tea extract ;  retina ; light damage ;  antioxidant ;  rat
DOI:
10.13389/j.cnki.rao.2015.0196
文献标志码:
A
摘要:
 目的　探讨岩茶提取物对大鼠视网膜光损伤的保护作用。方法　４０只ＳＤ大鼠随机分为４组：正常对照组、光损伤组、高剂量组（０．２０ｇ?Ｌ－１）和低剂量组（０．０５ｇ?Ｌ－１）。除正常对照组外，其余三组置于光照强度为（４０００±２００）Ｌｕｘ的光照箱内照射１２ｈ。高、低剂量组光照前３ｄ给予岩茶提取物尾静脉注射，每天一次，光照结束后继续给药７ｄ后处死。光镜下观察４组视网膜病理变化，检测视网膜组织外核层厚度、超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）活力及丙二醛（ｍａｌｏｎａｌｄｅｈｙｄｅ，ＭＤＡ）含量。结果　光损伤组光镜下视网膜各层结构疏松，外核层厚度变薄，见大量空泡细胞；感光细胞内外节排列紊乱，染色不均。高、低剂量组损伤均较光损伤组轻，其中高剂量组损伤更轻。与正常对照组（４１．０６±１．０１）μｍ比较，其余三组视网膜外核层厚度变薄（均为Ｐ＜０．０５），高、低剂量组较光损伤组（１５．１０±１．９２）μｍ厚，其中高剂量组（２５．７７±１．０８）μｍ较低剂量组（１９．２４±０．５５）μｍ厚（Ｐ＜０．０５）。与正常对照组相比，其余三组视网膜组织ＳＯＤ活力下降（Ｐ＜０．０５），ＭＤＡ含量升高（Ｐ＜０．０５）；与光损伤组比较，高、低剂量组视网膜ＳＯＤ活力升高（Ｐ＜０．０５），ＭＤＡ含量下降（Ｐ＜０．０５），高剂量组表现更明显（Ｐ＜０．０５）。结论　岩茶提取物对大鼠视网膜光损伤有一定的保护作用，且具有剂量依赖性。
Abstract:
 Objective To investigate the protective effects of rock tea extract on light-induced retinal damage in rats. Methods Forty Sprague-Dawley ( SD) rats were randomly divided int0 4 groups : Normal control group , light damage group , high dose (0.20 g . L") and low dose (0. 05 g . L-’) group. Except for the normal group, the other three groups were exposed to constant white fluorescent lamp irradiation for 12 hours at the average illuminance (4000 + 200) Lux. Rock tea extract was given every day by caudal vein 3 days before molding until all the animals were killed. All the rats were killed 7 days after irradiation. The pathological changes of retina were observed under optical microscope. The thickness of outer nuclear layer ( ONL) , activity of superoxide dismutase ( SOD) and content of malonaldehyde ( MDA) were detected. Results Each layer of retinal structure in light damage group was loose , the thickness of ONL became thinner and some cells showed varying degrees of vacuolar degeneration. Inner and outer segment of the photoreceptor cells arranged in disorder and stairung was uneven. The high and low dose groups showed various degrees of improvement, the high dose group was better than the low dose group. Compared with normal group(41. 06 + 1. 01 ) ym , the thickness of ONL of other three groups became thinner ( P < 0. 05 ) ; Compared with the light damage group ( 15. 10 + 1. 92 ) ym, the high dose group ( 25. 77 + 1. 08) ym increased. and the high dose group was thicker than the low dose group ( 19. 24 +0. 55) ym (P < 0. 05 ) . Compared with normal group , SOD activity in retina of other three groups reduced while MDA increased ( all P < 0. 05 ) . Compared with light damage group, SOD activity in retina of the high and low dose groups increased and MDA reduced ( all P < 0. 05 ) . The changes in high group were obvious (P < 0. 05 ) . Conclusion Rock tea extract has the protective effects on light-induced retinal damage in rats , which shows the dose-dependent manner.

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备注/Memo

备注/Memo:
 云南省应用基础研究项目（编号：２０１１ＦＺ２７３）
更新日期/Last Update: 2015-07-31