[1]朱美江,任成达,蔡雯婷,等.外周血液中miRNA表达与老年性黄斑变性相关性研究[J].眼科新进展,2019,39(7):677-681.[doi:10.13389/j.cnki.rao.2019.0156]
　ZHU Mei-Jiang,REN Cheng-Da,CAI Wen-Ting,et al.Correlation between the expression of circulating miRNAs and age-related macular degeneration[J].Recent Advances in Ophthalmology,2019,39(7):677-681.[doi:10.13389/j.cnki.rao.2019.0156]
点击复制

外周血液中miRNA表达与老年性黄斑变性相关性研究/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
39卷
期数:
2019年7期
页码:
677-681
栏目:
应用研究
出版日期:
2019-07-05

文章信息/Info

Title:
Correlation between the expression of circulating miRNAs and age-related macular degeneration
作者:
朱美江; 任成达; 蔡雯婷; 梁秀玮; 金惠子; 张瑞玲; 刘畅; 范佳淇; 沈天怡; 于靖
200072　上海市，同济大学附属第十人民医院眼科
Author(s):
ZHU Mei-Jiang; REN Cheng-Da; CAI Wen-Ting; LIANG Xiu-Wei; JIN Hui-Zi; ZHANG Rui-Ling; LIU Chang; FAN Jia-Qi; SHEN Tian-Yi; YU Jing
Department of Ophthalmology，Tenth People’s Hospital of Tongji University，Shanghai 200072，China
关键词:
老年性黄斑变性; miRNA; 基因芯片技术; 病例分析
Keywords:
age-related macular degeneration; miRNA; microarray; case-control
分类号:
R774.5
DOI:
10.13389/j.cnki.rao.2019.0156
文献标志码:
A
摘要:
目的　检测微小核糖核酸（microribonucleicacids，miRNA）在老年性黄斑变性（age-related macular degeneration，AMD）患者中的表达，并探讨miRNA的表达量与AMD病程之间的关系。方法　选取2014年1月至2016年11月于同济大学附属第十人民医院眼科门诊就诊的AMD患者6例为试验组，并选取同期6名正常人为对照组，通过基因芯片技术检测两组血液中miRNA的表达量。扩大样本的病例对照研究中共纳入126例AMD患者和140名正常人，检测其血液样本中miRNA的表达，比较两组人群间miRNA的表达量差异。结果　通过基因芯片技术，在试验组与对照组间共检测出216个miRNA存在表达差异（均为P<0.05），与对照组相比，试验组中111个miRNA表达量上升，105个miRNA表达量下降，差异均有统计学意义（均为P<0.05）。扩大样本的病例对照研究结果表明，在AMD患者中，miR-27a-3p、miR-29b-3p、miR-195-5p的表达量显著上升，同时，湿性AMD患者血液中miR-27a-3p的表达量高于干性AMD患者，差异均有统计学意义（均为P<0.05）。结论　AMD患者外周血中miRNA表达量水平有明显变化，miR-27a-3p、miR-29b-3p、miR-195-5p可能成为AMD血清学诊断和预后的标志物。
Abstract:
Objective　To detect the expression of microribonucleic acid (miRNA) inpatients with age-related macular degeneration (AMD),and to investigate the relationship between the expression of miRNA and the progression of AMD.Methods　Totally six AMD patients as experimental group and six gender-matched control patients were included in our study in the outpatient department of the Tenth People’s Hospital of Tongji University between January 2014 and November 2016,miRNA expression was measured by Taqman between these two groups.Furthermore,the larger whole blood samples of 126 AMD patients and 140 control patients were obtained in a case-control study to detect the differences of miRNA expression in the two groups.Results　A total of 216 differentially expressed miRNAs were detected via gene chips microarray in experimental group and control group (all P<0.05).Compared with control group,the expression of 111 miRNAs increased and 105 miRNAs decreased in experimental group.The results showed that the expression of miR-27a-3p,miR-29b-3p and miR-195-5p were significantly higher in AMD patients in the expanded case-control study.Moreover,the level of miR-27a-3p was higher in patients with wet AMD compared to patients with dry AMD (all P<0.05).Conclusion　Circulating miRNA levels are significantly varied in AMD patients.Especially,miR-27a-3p,miR-29b-3p and miR-195-5p may be a marker of serological diagnosis and prognosis of AMD.

参考文献/References:

［1］　LUTTRULL J K，MARGOLIS B W.Functionally guided retinal protective therapy for dry age-related macular and inherited retinal degenerations：A pilot study［J］.Invest Ophthalmol Vis Sci，2016，57（1）：265-275.
［2］　EANDI C M，ALOVISI C，DE SANCTIS U，GRIGNOLO F M.Treatment for neovascular age related macular degeneration：The state of the art［J］.Eur J Pharmacol，2016，787：78-83.
［3］　VAN LOOKEREN CAMPAGNE M，STRAUSS E C，YASPAN B L.Age-related macular degeneration：Complement in action［J］.Immunobiology，2016，221（6）：733-739.
［4］　ZUO K，ZHI K，ZHANG X，LU C，WANG S，LI M，et al.A dysregulated microRNA-26a/EphA2 axis impairs endothelial progenitor cell function via the p38 MAPK/VEGF pathway［J］.Cell Pysiol Biochem，2015，35（2）：477-88.
［5］　KARALI M，PERSICO M，MUTARELLI M，CARISSIMO A，PIZZO M，SINGH MARWAH V，et al.High-resolution analysis of the human retina miRNome reveals isomiR variations and novel microRNAs［J］.Nucleic Acids Res，2016，44（4）：1525-1540.
［6］　WU J，WANG R，YE Z，SUN X，CHEN Z，XIA F，et al.Protective effects of methane-rich saline on diabetic retinopathy via anti-inflammation in a streptozotocin-induced diabetic rat model［J］.Biochem Biophys Res Commun，2015，466（2）：155-161.
［7］　BHATTACHARJEE S，ZHAO Y，DUA P，ROGAEV E I，LUKIW W J.microRNA-34a-mediated down-regulation of the microglial-enriched triggering receptor and phagocytosis-sensor TREM2 in age-related macular degeneration［J］.PLoS One，2016，11（3）：e0150211.
［8］　SUN Y，LIU Y，COGDELL D，CALIN G A，SUN B，KOPETZ S，et al.Examining plasma microRNA markers for colorectal cancer at different stages［J］.Oncotarget，2016，7（10）：11434-11449.
［9］　CHANG P Y，CHEN C C，CHANG Y S，TSAI W S，YOU J F，LIN G P，CHEN T W，et al.MioRNA-223 and microRNA-92a in stool and plasma samples act as complementary biomarkers to increase colorectal cancer detection［J］.Oncotarget，2016，7（9）：10663-10675.
［10］　LAURENCE S L，PAUL M，JOHANNA M S，FRANK G H，TIEN Y W.Age-related macular degeneration［J］.Lancet，2012，379（9827）：1728-1738.
［11］　REN X.Image characteristics of wet age-related macular degeneration by optical coherence tomography angiography［J］.Cont Med，2018，24（26）：149-151.
任旋.湿性老年性黄斑变性光相干断层扫描血管成像图像特征研究［J］.当代医学，2018，24（26）：149-151.
［12］　SMYTH G K.Linear models and empirical bayes methods for assessing differential expression in microarray experiments［J］.Stat Appl Genet Mol Biol，2004，3：Article3.
［13］　SINGH N，SRINIVASAN S，MURALIDHARAN V，ROY R，V J，RAMAN R.Prevention of age-related macular degeneration［J］.Asia Pac J Ophthalmol（Phila），2017，6（6）：520-526.
［14］　MACHALINSKA A，KAWA M P，MARLICZ W，MACHALINSKI B.Complement system activation and endothelial dysfunction in patients with age-related macular degeneration （AMD）：possible relationship between AMD and atherosclerosis［J］.Acta Ophthalmol，2012，90（8）：695-703.
［15］　WANG S，KOSTER K M，HE Y，ZHOU Q.miRNAs as potential therapeutic targets for age-related macular degeneration［J］.Future Med Chem，2012，4（3）：277-287.
［16］　ZHANG Y，ZHANG D，WANG F，XU D，GUO Y，CUI W.Serum miRNAs panel （miR-16-2*，miR-195，miR-2861，miR-497） as novel non-invasive biomarkers for detection of cervical cancer［J］.Sci Rep，2015，5：17942.
［17］　CHEN X，SHI K，WANG Y，SONG M，ZHOU W，TU H，et al.Clinical value of integrated-signature miRNAs in colorectal cancer：miRNA expression profiling analysis and experimental validation［J］.Oncotarget，2015，6（35）：37544-37556.

相似文献/References:

[1]周尚昆 唐由之 冯俊 陈有信 戴虹 王慧娟. 明睛颗粒治疗湿性老年性黄斑变性的临床研究[J].眼科新进展,2014,34(2):155.
[2]郑玲,冯光强.小梁细胞表达的miRNA研究新进展[J].眼科新进展,2014,34(11):1094.[doi:10.13389/j.cnki.rao.2014.0304]
　ZHENG Ling,FENG Guang-Qiang.Research advances in miRNA in human trabecular meshwork cells[J].Recent Advances in Ophthalmology,2014,34(7):1094.[doi:10.13389/j.cnki.rao.2014.0304]
[3]吕洋,侯慧媛,王雨生. miRNA在脉络膜新生血管发生发展中的作用研究进展[J].眼科新进展,2015,35(3):283.[doi:10.13389/j.cnki.rao.2015.0076]
　LV Yang,HOU Hui-Yuan,WANG Yu-Sheng. Research advances in role of miRNA in development of choroidal neovascularization[J].Recent Advances in Ophthalmology,2015,35(7):283.[doi:10.13389/j.cnki.rao.2015.0076]
[4]刘雪莲,白宁艳,李治清,等.视网膜脉络膜深层扫描在老年性黄斑变性患者诊断中的作用[J].眼科新进展,2015,35(10):952.[doi:10.13389/j.cnki.rao.2015.0259]
　LIU Xue-Lian,BAI Ning-Yan,LI Zhi-Qing,et al.Role of retinal choroidal deep scan in diagnosis of age-related macular degeneration[J].Recent Advances in Ophthalmology,2015,35(7):952.[doi:10.13389/j.cnki.rao.2015.0259]
[5]孙园园,郭大东,毕宏生.MicroRNA在葡萄膜炎发病过程中的调控作用研究进展[J].眼科新进展,2016,36(11):1082.[doi:10.13389/j.cnki.rao.2016.0289]
　SUN Yuan-Yuan,GUO Da-Dong,BI Hong-Sheng.Recent advances in regulatory effects of microRNA in pathogenesis of uveitis[J].Recent Advances in Ophthalmology,2016,36(7):1082.[doi:10.13389/j.cnki.rao.2016.0289]
[6]吴若欣,徐洁慧,温积全,等.玻璃体内注射康柏西普对渗出性老年性黄斑变性患者脉络膜厚度的影响[J].眼科新进展,2016,36(12):1172.[doi:10.13389/j.cnki.rao.2016.0312]
　WU Ruo-Xin,XU Jie-Hui,WEN Ji-Quan,et al.Effects of intravitreal conbercept injections on choroidal thickness in exudative age-related macular degeneration[J].Recent Advances in Ophthalmology,2016,36(7):1172.[doi:10.13389/j.cnki.rao.2016.0312]
[7]郝义,孙笑,刘玉秋,等.miRNA-146a在视网膜色素上皮细胞老化及老年性黄斑变性中的功能研究[J].眼科新进展,2017,37(2):117.[doi:10.13389/j.cnki.rao.2017.0031]
　HAO Yi,SUN Xiao,LIU Yu-Qiu,et al.Function of miR-146a in retinal pigment epithelial cells aging and age-related macular degeneration[J].Recent Advances in Ophthalmology,2017,37(7):117.[doi:10.13389/j.cnki.rao.2017.0031]
[8]赵永吉,丁秋爱,游志鹏.老年性黄斑变性免疫炎症机制的研究进展[J].眼科新进展,2017,37(11):1079.[doi:10.13389/j.cnki.rao.2017.0273]
　ZHAO Yong-Ji,DING Qiu-Ai,YOU Zhi-Peng.Advance in immune and inflammatory mechanisms of age-related macular degeneration[J].Recent Advances in Ophthalmology,2017,37(7):1079.[doi:10.13389/j.cnki.rao.2017.0273]
[9]余曦,田敏,何薇,等.叔丁基对苯二酚对糖尿病大鼠视网膜的保护作用及其机制研究[J].眼科新进展,2018,38(8):719.[doi:10.13389/j.cnki.rao.2018.0169]
　YU Xi,TIAN Min,HE Wei,et al.Protective effects of tert-butylhydroquinone on the retina of diabetic rats and its mechanisms[J].Recent Advances in Ophthalmology,2018,38(7):719.[doi:10.13389/j.cnki.rao.2018.0169]
[10]刘阳,兰长骏,廖萱.近视的表观遗传学研究[J].眼科新进展,2018,38(10):909.[doi:10.13389/j.cnki.rao.2018.0215]
　LIU Yang,LAN Chang-Jun,LIAO Xuan.Review progress on epigenetics of myopia[J].Recent Advances in Ophthalmology,2018,38(7):909.[doi:10.13389/j.cnki.rao.2018.0215]

更新日期/Last Update: 2019-07-01