[1]白梦天,康刚劲,徐曼华,等.生姜提取物对链脲佐菌素诱导的糖尿病大鼠晶状体的保护作用[J].眼科新进展,2018,38(5):425-429.[doi:10.13389/j.cnki.rao.2018.0099]
　BAI Meng-Tian,KANG Gang-Jin,XU Man-Hua,et al.Protective effects of zingiber officinalis extract on the lens of diabetic rats[J].Recent Advances in Ophthalmology,2018,38(5):425-429.[doi:10.13389/j.cnki.rao.2018.0099]
点击复制

生姜提取物对链脲佐菌素诱导的糖尿病大鼠晶状体的保护作用/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年5期
页码:
425-429
栏目:
实验研究
出版日期:
2018-05-05

文章信息/Info

Title:
Protective effects of zingiber officinalis extract on the lens of diabetic rats
作者:
白梦天; 康刚劲; 徐曼华; 李茂娇; 彭正红; 吴剑; 李韵
646000　四川省泸州市，西南医科大学附属医院眼科 （白梦天，康刚劲，徐曼华，李茂娇，彭正红，吴剑）；621700　四川省江油市，江油市人民医院肿瘤科（李韵）
Author(s):
BAI Meng-Tian; KANG Gang-Jin; XU Man-Hua; LI Mao-Jiao; PENG Zheng-Hong; WU Jian; LI Yun
Department of Ophthalmology，the Affiliated Hospital of Southwest Medical University（BAI Meng-Tian，KANG Gang-Jin，XU Man-Hua，LI Mao-Jiao，PENG Zheng-Hong，WU Jian），Luzhou 646000，Sichuan Province，China；Department of Oncology，Jiangyou People’s Hospital （LI Yun），Jiangyou 621700，Sichuan Province，China
关键词:
晶状体上皮细胞; 糖尿病性白内障; 凋亡; 氧化应激
Keywords:
lens epithelial cells; diabetic cataract; apoptosis; oxidative stress
分类号:
R776.1
DOI:
10.13389/j.cnki.rao.2018.0099
文献标志码:
A
摘要:
目的　探讨生姜提取物对链脲佐菌素（streptozotocin，STZ）诱导的糖尿病大鼠晶状体的保护作用。方法　60只SD大鼠随机分为A组（正常对照组）、B组（糖尿病组）、C1组（糖尿病+生姜灌胃50 mg·kg-1组）、C2组（糖尿病+生姜灌胃100 mg·kg-1组）、C3组（糖尿病+生姜灌胃300 mg·kg-1组），每组各12只。除A组腹腔注射等体积生理盐水外，其余各组均一次性腹腔注射STZ 65 mg·kg-1。动物模型建立成功后，立即予以C1、C2、C3组大鼠生姜提取物灌胃，A、B组给予等量生理盐水灌胃。观察大鼠成模前与成模后4周、8周、12周时血糖、晶状体变化情况。成模后4周、8周、12周分批处死大鼠并立即取出晶状体，ELISA检测晶状体醛糖还原酶（aldose reductase，AR）含量；Western blot检测糖基化终末产物（glycosylation end products，AGEs）的表达；超氧化物歧化酶（superoxide dismutase，SOD）活性测定试剂盒检测SOD活性，丙二醛（malondialdehyde，MDA）含量测定试剂盒检测MDA含量；TUNEL法检测晶状体上皮细胞（lens epithelial cells，LECs）凋亡率，扫描电镜观察晶状体超微结构变化。结果　糖尿病大鼠晶状体SOD活性呈下降趋势，差异均有统计学意义（均为P<0.05）。除A、C3组外，其余各组大鼠晶状体MDA含量变化在成模后4周、8周、12周时差异均有统计学意义（P=0.003、0.000、0.017）。B组大鼠AR持续性增高（P=0.003）。C1、C2、C3组大鼠晶状体AR含量呈下降趋势，差异均有统计学意义（P=0.007、0.000、0.000）。C1、C2、C3组大鼠晶状体AGEs表达情况差异均有统计学意义（均为P<0.05）。扫描电镜发现，B组大鼠晶状体皮质纤维破坏程度呈进行性加重，C1、C2、C3组大鼠皮质破坏程度随灌胃浓度的增加而呈减轻趋势。A组大鼠LECs凋亡率无明显差异性变化（P=0.191），其余大鼠LECs的凋亡呈现上升趋势，差异均有统计学意义 （均为P<0.05）。结论　生姜提取物灌胃可延迟糖尿病大鼠晶状体混浊时间并减慢其进展速度，并有一定的浓度依赖性，这可能是通过抑制醛糖还原酶活性、氧化应激反应、AGEs的产生以及晶状体上皮细胞凋亡等途径实现的。
Abstract:
Objective　To observe the protective mechanisms of zingiber officinalis extract on the lens of diabetic rats induced by streptozotocin （STZ）.Methods　Totally 60 SD rats were randomly divided into A group （normal control rats），B group （diabetic rats），C1 group （DM+50 mg·kg-1 ginger gavage），C2 group （DM+100 mg·kg-1 ginger gavage） and C3 group （DM+300 mg·kg-1 ginger gavage）.Each group had 12 rats.And rats in A group received intraperitoneal injection of the same volume of normal saline，while the other groups were intraperitoneally injected with 65 mg·kg-1 streptozotocin （STZ）.When the animal model was successfully established，both A group and B group were administered orally with normal saline，and the C1，C2 and C3 groups were administered orally with ginger rhizome extract.The changes in blood glucose and lens were observed every week.The rats were sacrificed in succession at 4 week，8 week，12 week，and the lens was removed immediately.The content of aldose reductase （AR） was detected by ELISA，and the expression of glycosylation end products （AGEs） was detected by Western blot.The superoxide dismutase （SOS） activity and malondialdehyde （MDA） content were also detected.Fluorescent Tunel staining was used to detect the apoptosis of the lens epithelial cells.And finally，scanning electron microscopy was used to observe the ultrastructural changes in the lens.Results　The activity of SOD in the lens of diabetic rats showed a decreasing trend with statistical significance （all P<0.05）.Changes in the content of MDA in the lens of rats in B，C1，C2 group were statistically significant in 4，8 and 12 weeks after successful modeling （all P<0.05），but no significant difference in A group and C3 group （both P>0.05）.The persistent increase of AR in group B （P=0.003）.The content of AR of the lens in C1，C2，C3 group showed a decreasing trend，and the differences were statistically significant （all P<0.05）.There were significant differences in the expression of AGEs in C1，C2 and C3 group （all P<0.05）.The degree of cortical fiber destruction in group B was progressively aggravated，but the degree of cortical destruction in C1，C2 and C3 group decreased with the increase of ginger gavage concentration through the scanning electron microscopy.It was observed that there was no significant difference in the apoptotic rate of LECs in A group （P=0.191），but the apoptosis of LECs in the rest group showed a rising trend，and the differences were statistically significant （all P<0.05）.Conclusion　The effects of ginger gavage extract can delay the opacification of lens and slow down the diabetic development in rats with a dose-independence manner.Ginger gavage extract may play a protective role on the lens of diabetic rats by inhibiting the activity of AR，oxidative stress and the production of AGEs as well as suppress the apoptosis of lens epithelial cells.

参考文献/References:

［1］　POLLREISZ A，SCHMIDT-ERFURTH U.Diabetic cataract pat-hogenesis，epidemiology and treatment［J］.J Ophthalmol，2010，2010：608751.
［2］　TZENG TF，LIOU SS，TZENG YC，LIU IM.Zerumbone，a phytochemical of subtropical ginger，protects against hyperglycemia-induced retinal damage in experimental diabetic rats［J］.Nutrients，2016，8（8）：449.
［3］　SARASWAT M，SURYANARAYANA P，REDDY PY，PATIL MA，BALAKRISHNA N，REDDY GB.Antiglycating potential of Zingiber officinalis and delay of diabetic cataract in rats［J］.Mol Vis，2010，16：1525-1537.
［4］　AO S，KIKUCHI C，ONO T，NOTSU Y.Effect of instillation of aldose reductase inhibitor FR74366 on diabetic cataract［J］.Invest Ophthalmol Vis Sci，1991，32（12）：3078-3083.
［5］　YANG CX，YAN H，LIU Q，GUO Y.Inhibitive effect of saturated hydrogen saline on sodium selenite induced cataract in rats［J］.Rec Adv Ophthalmol，2013，33（4）：305-308.
杨春潇，严宏，刘擎，郭勇.氢饱和生理盐水对大鼠亚硒酸钠性白内障的抑制作用［J］.眼科新进展，2013，33（4）：305-308.
［6］　OJEWOLE JA.Analgesic antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale （Roscoe） rhizomes （Zingiberaceae） in mice and rats［J］.Phytother Res，2006，20（9）：764-772.
［7］　RANI MP，KRISHNA MS，PADMAKUMARI KP，RAGHU KG，SUNDARESAN A.Zingiber officinale extract exhibits antidiabetic potential via modulating glucose uptake，protein glycation and inhibiting adipocyte differentiation：an in vitro study［J］.J Sci Food Agric，2012，92（9）：1948-1955.
［8］　SHANMUGAM KR，MALLIKARJUNA K，NISHANTH K，KUO CH，REDDY KS.Protective effect of dietary ginger on antioxidant enzymes and oxidative damage in experimental diabetic rat tissues［J］ Food Chem，2011，124（4）：1436-1442.
［9］　AKHANI SP，VISHWAKARMA SL，GOYAL RK.Anti-diabetic activity of Zingiber officinale in streptozotocin-induced type I diabetic rats［J］.J Pharm Pharmacol，2004，56（1）：101-105.
［10］　HEIMES K，FEISTEL B，VERSPOHL EJ.Impact of the 5-HT3 receptor channel system for insulin secretion and interaction of ginger extracts［J］.Eur J Pharmacol，2009，624（1-3）：58-65.
［11］　LUPACHYK S，STAVNIICHUK R，KOMISSARENKO JI，DREL VR，OBROSOV AA，EI-REMESS AB，et al.Na+/H+-exchanger-1 inhibition counteracts diabetic cataract formation and retinal oxidative-nitrative stress and apoptosis［J］.Int J Mol Med，2012，29（6）：989-998.
［12］　LIU J，KANG GJ，KANG HJ，BAI MT，JIANG Y，HAN Q.Quercetin on apoptosis of lens epithelial cells in diabetic rats［J］.J Luzhou Med Coll，2016，39（6）：560-565.
刘鹃，康刚劲，康海军，白梦天，蒋燕，韩茜.槲皮素对糖尿病大鼠晶状体上皮细胞凋亡的影响［J］.泸州医学院学报，2016，39（6）：560-565.
［13］　WU J，LI X，WAN WC，YANG QH，MA WF，CHEN D，et al.Gigantol from Dendrobium chrysotoxum Lindl.binds and inhibits aldose reductase gene to exert its anti-cataract activity：An in vitro mechanistic study［J］.J Ethnopharmacol，2017，198：255-261.
［14］　SANKESHI V，KUMAR PA，NAIK RR，SRIDHAR G，KUMAR MP，GOPAL VV，et al.Inhibition of aldose reductase by Aegle marmelos and its protective role in diabetic cataract［J］.J Ethnopharmacol，2013，149（1）：215-221.
［15］　LI X，LIU WP，HUANG XD，XIONG JP，WEI XY.Interaction of AR and iNOS in lens epithelial cell：A new pathogenesis and potential therapeutic targets of diabetic cataract［J］.Arch Biochem Biophys，2017，615：44-52.
［16］　HEGDE KR，HENEIN MG，VARMA SD.Establishment of mouse as an animal model for study of diabetic cataracts：biochemical studies［J］.Diabetes Obes Metab，2003，5（2）：113-119.
［17］　THIRAPHATTHANAVONG P，WATTANATHORN J，MUCHIMAPURA S，THUKHAM-MEE W，LERTRAT K，SURIHARN B.The combined extract of purple waxy corn and ginger prevents cataractogenesis and retinopathy in streptozotocin-diabetic rats［J］.Oxid Med Cell Longev，2014，2014：789406.
［18］　CHIEN TY，CHEN LG，LEE CJ，LEE FY，WANG CC.Anti-inflammatory constituents of Zingiber zerumbet［J］.Food Chem，2008，110（3）：584-589.
［19］　FRANKE S，DAWCZYNSKI J，STROBEL J，NIWA T，STAHL P，STEIN G.Increased levels of advanced glycation end products in human cataractous lenses［J］.J Cataract Refract Surg，2003，29（5）：998-1004.

相似文献/References:

[1]丁文君 韦琦 梁皓 陈金卯 李霞 谭少健.糖尿病兔后发性白内障发生过程中晶状体上皮细胞增殖的变化[J].眼科新进展,2012,32(1):000.
[2]邢星 胡义珍 李世洋 马红利.tTG 小干扰 RNA 对人晶状体上皮细胞转分化和细胞外基质沉积的抑制作用[J].眼科新进展,2012,32(1):000.
[3]朱艳 朱玉广 张立华 钟莹莹 杜孝楠 张荣 王杰.整合素β1介导的ERK/MAPK通道在人晶状体上皮细胞转分化中的作用[J].眼科新进展,2012,32(4):000.
[4]钟晓东 罗卫民 刘越峰.白藜芦醇对糖尿病性白内障大鼠晶状体上皮细胞凋亡及 bcl-2和 bax表达的影响[J].眼科新进展,2012,32(7):000.
[5]刘丹 门保成 张姝.茶多酚对H2O2诱导大鼠晶状体上皮细胞中Caveolin-1表达的影响[J].眼科新进展,2013,33(7):000.
[6]王新 李宝华 张明昌 张向东.表基因修饰参与H2O2介导人晶状体上皮细胞SRA01/04的生长停滞[J].眼科新进展,2013,33(8):000.
[7]钟晓东 罗卫民 刘越峰 余锦强 李凌 王莹 李玉霞.白藜芦醇对大鼠糖尿病性白内障抗氧化损伤的保护作用[J].眼科新进展,2012,32(2):000.
[8]丁芝祥 彭燕一 邱梅园 张玉明 曾新生.腺相关病毒介导HSV-tk/GCV 系统对晶状体上皮细胞的旁观者效应及机制[J].眼科新进展,2012,32(8):000.
[9]吕莎 宋胜仿 李华 徐霁 苏杨 吴杨杨.糖尿病性白内障术后角膜内皮细胞的变化[J].眼科新进展,2012,32(8):000.
[10]陈玮.年龄相关性白内障晶状体上皮细胞中bFGF、α-SMA的表达及临床意义[J].眼科新进展,2012,32(9):000.
[11]刘擎 杨烨 严宏. 高糖对人晶状体上皮细胞硫醇转移酶表达的影响[J].眼科新进展,2014,34(3):201.
[12]刘玉梅兰,杜珊珊,邵敬芝,等.晚期糖基化终末产物受体（RAGE）在糖尿病性白内障和年龄相关性白内障患者前囊膜及人晶状体上皮细胞中的表达[J].眼科新进展,2020,40(11):1015.[doi:10.13389/j.cnki.rao.2020.0227]
　LIU Yumeilan,DU Shanshan,SHAO Jingzhi,et al.Expression of RAGE in the anterior capsule of diabetic cataract and human LECs[J].Recent Advances in Ophthalmology,2020,40(5):1015.[doi:10.13389/j.cnki.rao.2020.0227]
[13]陈晓娟,曹鑫,薛理丹,等.高迁移率族蛋白B1对高糖诱导的晶状体上皮细胞凋亡和自噬的影响[J].眼科新进展,2023,43(1):013.[doi:10.13389/j.cnki.rao.2023.0003]
　CHEN Xiaojuan,CAO Xin,XUE Lidan,et al.Effects of high mobility group box 1 protein on apoptosis and autophagy of lens epithelial cells induced by high glucose[J].Recent Advances in Ophthalmology,2023,43(5):013.[doi:10.13389/j.cnki.rao.2023.0003]
[14]任若昕,管怀进,季敏.晶状体上皮细胞葡萄糖代谢的研究进展[J].眼科新进展,2023,43(5):412.[doi:10.13389/j.cnki.rao.2023.0083]
　REN Ruoxin,GUAN Huaijin,JI Min.Research progress in glucose metabolism of lens epithelial cell[J].Recent Advances in Ophthalmology,2023,43(5):412.[doi:10.13389/j.cnki.rao.2023.0083]

备注/Memo

备注/Memo:
西南医科大学附属医院国际合作课题（编号：2011-43）
更新日期/Last Update: 2018-05-09