[1]赵佐芳,王大庆,王宁,等.骨膜蛋白在眼睑基底细胞癌相关成纤维细胞与眼睑正常成纤维细胞中的表达差异[J].眼科新进展,2018,38(5):430-433.[doi:10.13389/j.cnki.rao.2018.0100]
　ZHAO Zuo-Fang,WANG Da-Qing,WANG Ning,et al.Expression differences and significance of periostin in eyelid basal cell carcinoma associated fibroblasts andnormal fibroblasts[J].Recent Advances in Ophthalmology,2018,38(5):430-433.[doi:10.13389/j.cnki.rao.2018.0100]
点击复制

骨膜蛋白在眼睑基底细胞癌相关成纤维细胞与眼睑正常成纤维细胞中的表达差异/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年5期
页码:
430-433
栏目:
实验研究
出版日期:
2018-05-05

文章信息/Info

Title:
Expression differences and significance of periostin in eyelid basal cell carcinoma associated fibroblasts andnormal fibroblasts
作者:
赵佐芳; 王大庆; 王宁; 雷小平; 朱大勇; 张艳红; 税民鸿; 贾前伟; 程阳; 马善春; 田翠玲; 连刚; 涂静; 王定松; 李维
611730　四川省成都市，郫都区人民医院眼科（赵佐芳，雷小平，朱大勇，张艳红，税民鸿，贾前伟，程阳，马善春，田翠玲，连刚，涂静，王定松，李维）；637000　四川省南充市，川北医学院附属医院眼科，川北医学院眼视光学系（王大庆，王宁）
Author(s):
ZHAO Zuo-Fang; WANG Da-Qing; WANG Ning; LEI Xiao-Ping; ZHU Da-Yong; ZHANG Yan-Hong; SHUI Min-Hong; JIA Qian-Wei; CHENG Yang; MA Shan-Chun; TIAN Cui-Ling; LIAN Gang; TU Jing; WANG Ding-Song; LI Wei
Department of Ophthalmology，Pidu District People’s Hospital（ZHAO Zuo-Fang，LEI Xiao-Ping，ZHU Da-Yong，ZHANG Yan-Hong，SHUI Min-Hong，JIA Qian-Wei，CHENG Yang，MA Shan-Chun，TIAN Cui-Ling，LIAN Gang，TU Jing，WANG Ding-Song，LI Wei），Chengdu 611730，Sichuan Province，China；Department of Ophthalmology，the Affiliated Hospital of North Sichuan Medical College，Department of Eye Optical of North Sichuan Medical College（WANG Da-Qing，WANG Ning），Nanchong 637000，Sichuan Province，China
关键词:
骨膜蛋白; 眼睑基底细胞癌; 癌相关成纤维细胞; 正常成纤维细胞
Keywords:
periostin; eyelid basal cell carcinoma; carcinoma associated fibroblasts; normal fibroblasts
分类号:
R777.1
DOI:
10.13389/j.cnki.rao.2018.0100
文献标志码:
A
摘要:
目的　比较对已分离、培养、纯化、鉴定的眼睑基底细胞癌相关成纤维细胞（basal cell carcinoma associated fibroblasts，BCAFs）与眼睑正常成纤维细胞（normal fibroblasts，NFs）中骨膜蛋白（periostein，PN）表达差异。方法　取生长良好的第3代纯化的BCAFs和NFs，用胰蛋白酶调制细胞悬液的浓度均为20×103个·mL-1，按每孔2 mL细胞悬液接种六孔板。以无血清培养基培养48 h后收集上清液，通过酶联免疫吸附试验（ELISA）检测BCAFs与NFs上清液中PN的含量；以盖玻片置于六孔板底制作细胞爬片，应用免疫荧光细胞化学染色技术检测BCAFs与NFs中PN的表达。结果　眼睑BCAFs与NFs上清液中PN的含量分别为（9.26±2.35）μg·L-1和（2.57±0.41）μg·L-1；细胞免疫荧光化学染色检测PN表达显示，眼睑BCAFs中强于NFs，差异有统计学意义（P<0.05）。结论　眼睑BCAFs较NFs高表达分泌PN，提示PN在眼睑基底细胞癌的局部侵袭生长中起着一定作用。
Abstract:
Objective　To investigate the expression differences and significance of periostin （PN） in eyelid basal cell carcinoma associated fibroblasts （BCAFs） andnormal fibroblasts （NFs） after separation，culture，purification and identification.Methods　The third generation of purified BCAFs and NFs was selected，and the concentrations of cell suspensions were modulated to 20×106 L-1 by trypsin，and then the cell suspension were seeded and cultured in 6-well plate by 2 mL per well.The cell culture supernatants were collected when BCAFs and NFs were cultured by serum-free medium for 48 h，then the content of PN in cell culture supernatants from BCAFs and NFs was detected by enzyme-linked immunosorbent assay （ELISA）.The glass coverslips were placed at the bottom of the 6-well plate to make cell slides，and then the expression of PN in BCAFs and NFs cells were tested by immunofluorescence staining.Results　ELISA showed that the content of PN in cell culture supernatants from BCAFs and NFs was （9.26±2.35）μg·L-1 and （2.57±0.41）μg·L-1.And the expression level of PN in BCAFs tested by immunofluorescence staining technology was higher than that in NFs cells，and the differences were statistically significant （all P<0.05）.Conclusion The expression and secretion of PN in the eyelid BCAFs were highly enhanced when compared with NFs，suggesting that periostein may promote or inhibit the occurrence and development of the eyelid basal cell carcinoma in the microenvironment of the eyelid basal cell carcinoma.

参考文献/References:

［1］　LI FM，XIE LX.Chinese ophthalmology［M］.3rd ed.Beijing：People’s Medical Publishing House，2014：916-918.
李凤鸣，谢立信.中华眼科学［M］.第3版.北京：人民卫生出版社，2014：916-918.
［2］　SCHMITT-GRAFF A，DESMOULIERE A，GABBIANI G.Heterogeneity of myofibroblast phenotypic features：an example of fibroblastic cell plasticity［J］.Virchows Arch，1994，425（1）：3-24.
［3］　SOLTERMANN A，OSSOLA R，KILGUS-HAWELSHI S，VON ECKARDSTEIN A，SUTER T，AEBERSOLD R，et al.N-glycoprotein profiling of lung adenocarcinoma pleural effusions by shotgun proteomics［J］.Cancer，2008，114（2）：124-133.
［4］　CONWAY SJ，IZUHARA K，KUDO Y，LITVIN J，MARKWALD R，OUYANG G，et al.The role of periostin in tissue remodeling across health and disease［J］.Cell Mol Life Sci，2014，71（7）：1279-1288.
［5］　ZHAO ZF，WANG N，WANG DQ，LAN CJ.Differences in cellular morphology and proliferation of eyelid basal cell carcinoma associated fibroblasts and normal fibroblasts［J］.Rec Adv Ophthalmol，2016，36（4）：327-330.
赵佐芳，王宁，王大庆，兰长骏.眼睑基底细胞癌相关成纤维细胞与正常成纤维细胞形态与生长增殖特性差异［J］.眼科新进展，2016，36（4）：327-330.
［6］　NORRIS RA，DAMON B，MIRONOV V，KASYANOV V，RAMA-MURTHI A，MORENO-RODRIGUEZ R，et al.Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues［J］.J Cell Biochem，2007，101（3）：695-711.
［7］　MA D，LU H，XU L，XU X，XIAO W.Mechanical loading promotes Lewis lung cancer cell growth through periostin［J］.In Vitro Cell Dev Biol Anim，2009，45（8）：467-472.
［8］　OUYANG GL，LIU M，RUAN K，SONG G，MAO Y，BAO S.Upregulated expression of periostin by hypoxia in non-small-cell lung cancer cells promotes cell survival via the Akt/PKB pathway［J］.Cancer Lett，2009，281（2）：213-219.
［9］　BAO S，OUYANG G，BAI X，HUANG Z，MA C，LIU M，et al.Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway［J］.Cancer Cell，2004，5（4）：329-339.
［10］　MORRA L，MOCH H.Periostin expression and epithelial-mesenchymal transition in cancer：a review and an update［J］.Virchows Arch，2011，459（5）：465-475.
［11］　ZHAO ZF，LIU Y，WANG DQ.Advances in the role of periostin in tumor［J］.Chongqing Med J，2016，45（32）：4581-4584.
赵佐芳，刘英，王大庆.骨膜蛋白在肿瘤中的作用研究进展［J］.重庆医学，2016，45（32）：4581-4584.
［12］　TILMAN G，MATTIUSSI M，BRASSEUR F，Van BAREN N，DECOTTIGNIES A.Human periostin gene expression in normal tissues，tumors and melanoma：evidences for periostin production by both stromal and melanoma cells［J］.Mol Cancer，2007，6：80.
［13］　ERKAN M，KLEEFF J，GORBACHEVSKI A，REISER C，MITKUS T，ESPOSITO I，et al.Periostin creates a tumor-supportive microenvironment in the pancreas by sustaining fibrogenic stellate cell activity［J］.Gastroenterology，2007，132（4）：1447-1464.
［14］　KIKUCHI Y，KUNITA A，IWATA C，KOMURA D，NISHIYAMA T，SHIMAZU K，et al.The niche component periostin is produced by cancer-associated fibroblasts，supporting growth of gastic cancer through ERK activation［J］.Am J Pathol，2014，184（3）：859-870.

相似文献/References:

[1]赵佐芳,王宁,王大庆,等.眼睑基底细胞癌相关成纤维细胞与正常成纤维细胞形态与生长增殖特性差异[J].眼科新进展,2016,36(4):327.[doi:10.13389/j.cnki.rao.2016.0088]
　ZHAO Zuo-Fang,WANG Ning,WANG Da-Qing,et al.Differences in cellular morphology and proliferation of eyelid basal cell carcinoma associated fibroblasts and normal fibroblasts[J].Recent Advances in Ophthalmology,2016,36(5):327.[doi:10.13389/j.cnki.rao.2016.0088]
[2]刘舒静,万光明,梁申芝,等.血清骨膜蛋白、血管内皮生长因子与糖尿病视网膜病变的关系[J].眼科新进展,2018,38(6):563.[doi:10.13389/j.cnki.rao.2018.0132]
　LIU Shu-Jing,WAN Guang-Ming,LIANG Shen-Zhi,et al.Association of diabetic retinopathy with serum periostin and vascular endothelial growth factor[J].Recent Advances in Ophthalmology,2018,38(5):563.[doi:10.13389/j.cnki.rao.2018.0132]

备注/Memo

备注/Memo:
四川省卫生和计划生育委员会科研课题（编号：17PJ549）
更新日期/Last Update: 2018-05-09