[1]李妍,李丽红,刘海,等.树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化[J].眼科新进展,2017,37(9):824-827.[doi:10.13389/j.cnki.rao.2017.0208]
 LI Yan,LI Li-Hong,LIU Hai,et al.Changes of Th1/Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis[J].Recent Advances in Ophthalmology,2017,37(9):824-827.[doi:10.13389/j.cnki.rao.2017.0208]
点击复制

树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化/HTML
分享到:

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年9期
页码:
824-827
栏目:
实验研究
出版日期:
2017-09-05

文章信息/Info

Title:
Changes of Th1/Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis
作者:
李妍李丽红刘海盛智超闵婕胡竹林
650000 云南省昆明市,昆明医科大学第四附属医院眼科、云南省第二人民医院眼科、云南省眼科研究所、云南省眼科疾病防治研究重点实验室、云南省第二人民医院白内障与眼底疾病防治省创新团队(李妍,刘海,盛智超,闵婕,胡竹林);650000 云南省昆明市,中国科学院昆明动物研究所动物模型与人类疾病机理重点实验室(李丽红)
Author(s):
LI YanLI Li-HongLIU HaiSHENG Zhi-ChaoMIN JieHU Zhu-Lin
Department of Ophthalmology,the 4th Affiliated Hospital of Kunming Medical University(LI Yan,LIU Hai,SHENG Zhi-Chao,MIN Jie,HU Zhu-Lin),Kunming 650000,Yunnan Province,China;Key Laboratory of Animal Models and Human Disease Mechanisms,Kunming Institute of Zoology,Chinese Academy of Sciences(LI Li-Hong),Kunming 650000,Yunnan Province,China
关键词:
树鼩茄病镰刀菌细胞因子
Keywords:
tree shrewsfusarium solanicytokines
分类号:
R772.21
DOI:
10.13389/j.cnki.rao.2017.0208
文献标志码:
A
摘要:
目的 观察树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化,了解Th1/Th2炎性因子与本病炎症反应的关系。方法 茄病镰刀菌培养7 d后收集真菌混悬液,调整孢子密度为10×109 CFU·mL-1。清洁级树鼩40只随机分为实验组30只、对照组10只,右眼为实验眼。实验组将真菌孢子混悬液50 μL注入角膜基质中央,对照组注入生理盐水50 μL。造模后第3天、第7天、第14天,流式细胞仪分析房水中白细胞介素(interleukin,IL)-1β、IL-6、IL-4、IL-10的水平;病理学检查观察浸润细胞类型。结果 Th1型细胞因子IL-1β和IL-6浓度均在造模后第7天达到高峰,造模后各时间点与对照组相比差异均具有统计学意义(均为P<0.05);Th2细胞因子IL-10浓度在造模后第14天达到高峰,造模后第7天、第14天与对照组相比差异均具有统计学意义(均为P<0.05);IL-4浓度仅在造模后第7天与对照组相比差异有统计学意义(P<0.05)。病理学检查结果示:炎症细胞浸润数量造模后第7天达到高峰,以中性粒细胞为主;实验组各时间点均可见菌丝平行于基质纤维生长。结论 促炎因子IL-1β和IL-6及抑炎因子IL-10在树鼩茄病镰刀菌性角膜炎炎症反应机制中发挥重要作用。
Abstract:
Objective To observe the changes of Th1 / Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis,as well as to explore the relationship between Th1 / Th2 inflammatory factors and inflammatory response in fusarium solani keratitis.Methods Forty healthy tree shrews were randomly divided into experimental group(n=30) and control group (n=10).Fusarium solani was inoculated into sabina culture medium and cultured at 26 ℃ for 7 days,and then the fungal suspension was collected and the density of spores was adjusted to 10×109 CFU·mL-1.In the experimental group,50 μL fungal spore suspension was injected into the center of the cornea stroma,while the control group received the same amount of saline.Next the levels of cytokines including interleukin (IL)-1β,IL-6,IL-4 and IL-10 were analyzed by flow cytometry on day 3,day 7,day 14 after successful modeling,and the changes in types of infiltrating cells were observed by histopathological examination.Results The expression level of IL-1β and IL-6 (Th1 type cytokines) was the highest on day 7,and the difference was statistically significant at each time point when compared with the control group (all P<0.05).The expression level of IL-10 (Th2 type cytokines) was the highest on day 14,and the difference was statistically significant at each time point when compared with the control group (all P<0.05).The difference in IL-4 expression was statistically significant on day 7 (P<0.05).In addition,histopathological examination showed that the number of infiltration cell reached its peak on day 7,mainly neutrophils,and fungal hyphae were observed to be parallel to the matrix fibers at each time point.Conclusion The proinflammatory cytokines IL-1β and IL-6 and the anti-inflammatory cytokines IL-10 may play an important role in the molecular mechanism of inflammatory response of fusarium solani keratitis in tree shrews.

参考文献/References:

[1] WU H,LIU S,WIRADHARMA N,ONG ZY,LI Y,YANG YY,et al.Short synthetic α-helical-forming peptide amphiphiles for fungal keratitis treatment in vivo[J].Adv Healthc Mater,2017,6(6):1-7.
[2] SIMEONE CA,TRAVERSI JP,MEEGAN JM,LEBERT C,COLITZ CM,JENSEN ED.Clinical management of Candida albicans keratomycosis in a bottlenose dolphin(Tursiops truncatus)[J].Vet Ophthalmol,2017,11(1):1-7.
[3] 陈立新,陈勇,宰建国.多层羊膜移植治疗真菌性角膜溃疡疗效观察[J].新乡医学院学报,2005,22(3):258-259.
CHEN LX,CHEN Y,ZAI JG.Observation of curative effect of fungal corneal ulcer treated by multilayer amniotic membrane transplantation[J].J Xinxiang Med Coll,2005,22(3):258-259.
[4] BANSAL Y,CHANDER J,KAISTHA N,AINGLA N,SOOD S,VAN DIEPENINGEN AD.Fusarium sacchari,a cause of mycotic keratitis among sugarcane farmers-a series of four cases from North India[J].Mycoses,2016,59(11):705-709.
[5] 穆红梅,皮百木,李家臣,张婉婷,郑娜,李娟.真菌性角膜炎的流行病学、临床和微生物学特征分析[J].眼科新进展,2013,33(9):879-880.
MU HM,PI BM,LI JC,ZHANG WT,ZHENG N,LI J.Epidemiological,clinical and microbiological features of fungal keratitis[J].Rec Adv Ophthalmol,2013,33(9):879-880.
[6] VEMUGANTI GK,GARG P,GOPINATHAN U,NADUVILATH TJ,JOHN RK,BUDDI R,et al,Evaluation of agent and host factors I progression of mycotic keratitis:A histologic and microbiologi Study of 167 cornea Buttons[J].Ophthalmology,2002,109(8):1538-1546.
[7] THOMAS PA.Mycotick eratitis-an underestimated mycosis[J].J Med Vet Mycol,1994,32(4):235-256.
[8] SUN M,ZHU M,CHEN K,NIE X,DENG Q,HAZLETT LD,et al.TREM-2 promotes host resistance against pseudomonas aeruginosa infection by suppressing corneal inflammation via a PI3K/Akt signaling pathway[J].Invest Ophthalmol Vis Sci,2013,54(5):3451-3462.
[9] ZHANG H,LI H,LI Y,ZOU Y,DONG X,SONG W,et al.IL-17 plays a central role in initiating experimental Candida albicans infection in mouse corneas[J].Eur J Immunol,2013,43(10):2671-2682.
[10] ZHONG W,YIN H,XIE L.Expression and potential role of major inflammatory cytokines in experimental keratomycosis[J].Mol Vis,2009,15:1303-1311.
[11] MATSUMOTO K,IKEMA K,JANIHARA H.Role of cytokines and chemokines in pseudomonal keratitis[J].Cornea,2005,24(8):43-49.
[12] NISHIMOTO N,KISHIMOTO T.Interleukin 6:From bench to bedside[J].Nat Clin Pract Rheumatol,2006,2(11):619-626.
[13] COLE N,BAO S,STAPLETON F,THAKUR A,HUSBAND AJ,BEAGLEY KW,et al.Pseudomonas aeruginosa keratitis in IL-6-deficient mice[J].Int Arch Allergy Immunol,2003,130(2):165-172.
[14] ROBERTS-THOMSON IC,FON J,UYLAKI W,CUMMINS AG,BARRY S.Cells,cytokines and inflammatory bowel disease:a clinical perspective[J].Expert Rev Gastroenterol Hepatol,2011,5(6):703-716.
[15] ZENEWICZ LA,ANTOY A,FLAVELL RA.CD4 T-cell differentiation and inflammatory bowel disease[J].Trends Mol Med,2009,15(5):199-207.
[16] 陈丽,谢正德.白细胞介素-10与EB病毒相关疾病的关系研究进展[J].中华实用儿科临床志,2017,32(10):791-793.
CHEN L,XIE ZD.Research progress in the relationship between interleukin-10 and Epstein-Barrvirusasso-ciated disease[J].Chin J Appl Clin Pediatr,2017,32(10):791-793.

相似文献/References:

[1]朱然,梁舒,钱晶晶,等.高糖和糖浓度波动对人角膜上皮细胞中MMP-9表达及真菌黏附的影响[J].眼科新进展,2021,41(6):534.[doi:10.13389/j.cnki.rao.2021.0111]
 ZHU Ran,LIANG Shu,QIAN Jingjing,et al.Effects of high glucose and fluctuating glucose on the expression of MMP-9 in human corneal epithelium cells and the adhesion of Fungi[J].Recent Advances in Ophthalmology,2021,41(9):534.[doi:10.13389/j.cnki.rao.2021.0111]
[2]李豪亮,朱然,张希熹,等.茄病镰刀菌对人角膜上皮细胞NOD2表达及相关炎症因子分泌水平的影响[J].眼科新进展,2021,41(7):633.[doi:10.13389/j.cnki.rao.2021.0130]
 LI Haoliang,ZHU Ran,ZHANG Xixi,et al.Effect of Fusarium solani on the expression of NOD2 and the secretion of related inflammatory factors in human corneal epithelial cells[J].Recent Advances in Ophthalmology,2021,41(9):633.[doi:10.13389/j.cnki.rao.2021.0130]
[3]李妍,李丽红,戴敏,等.真菌性角膜炎角膜组织NOD1、NF-κBp65表达的变化以及炎症细胞浸润情况[J].眼科新进展,2023,43(2):089.[doi:10.13389/j.cnki.rao.2023.0018]
 LI Yan,LI Lihong,DAI Min,et al.Changes in expression of nucleotide-binding oligomerization domain-containing protein 1 and nuclear factor kappa-Bp65 and infiltration of inflammatory cells in the cornea with fungal keratitis[J].Recent Advances in Ophthalmology,2023,43(9):089.[doi:10.13389/j.cnki.rao.2023.0018]
[4]赵秋语,陈黎,胡敏.树鼩形觉剥夺性弱视模型的视网膜形态变化[J].眼科新进展,2023,43(7):520.[doi:10.13389/j.cnki.rao.2023.0105]
 ZHAO Qiuyu,CHEN Li,HU Min.Retinal morphological changes in tree shrew model with form deprivation amblyopia[J].Recent Advances in Ophthalmology,2023,43(9):520.[doi:10.13389/j.cnki.rao.2023.0105]

备注/Memo

备注/Memo:
云南省卫生科技计划项目(编号:2016NS182、2017NS129);昆明医科大学博士创新基金(编号:2016D07)
更新日期/Last Update: 2017-10-12