[1]陈星如,高卫萍.针刺对干眼兔泪腺形态学及泪腺上皮细胞凋亡相关基因蛋白表达的影响[J].眼科新进展,2017,37(3):210-214.[doi:10.13389/j.cnki.rao.2017.0054]
　CHEN Xing-Ru,GAO Wei-Ping.Effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbits[J].Recent Advances in Ophthalmology,2017,37(3):210-214.[doi:10.13389/j.cnki.rao.2017.0054]
点击复制

针刺对干眼兔泪腺形态学及泪腺上皮细胞凋亡相关基因蛋白表达的影响/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年3期
页码:
210-214
栏目:
实验研究
出版日期:
2017-03-05

文章信息/Info

Title:
Effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbits
作者:
陈星如; 高卫萍
210029　江苏省南京市，南京中医药大学
Author(s):
CHEN Xing-Ru; GAO Wei-Ping
Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu Province,China
关键词:
针刺; 干眼; 泪腺; 细胞凋亡
Keywords:
acupuncture; dry eye; lacrimal gland; cell apoptosis
分类号:
R777.2
DOI:
10.13389/j.cnki.rao.2017.0054
文献标志码:
A
摘要:
目的　探讨针刺对干眼兔泪腺形态学及泪腺上皮细胞凋亡相关基因蛋白表达的影响，分析针刺对干眼的作用机制。方法　取健康新西兰白兔18只随机分为空白组、模型组、针刺组，每组6只，空白组不作任何处理；模型组皮下注射氢溴酸东莨菪碱，每天4次，每次2 mg·kg-1共24 d；针刺组在与模型组同样处理的基础上进行针刺，每天1次，共14 d。不同时段观察各组泪液分泌试验检测泪液量的结果，光镜、电镜观察泪腺形态学变化，免疫组织化学检测泪腺组织中凋亡相关基因蛋白的表达。结果　泪液分泌试验检测泪液量结果显示在针刺第4、7、14天同时段针刺组与模型组相比明显升高，差异均有统计学意义（均为P＜0.05）；在针刺第14天时，针刺组与实验前相比，差异无统计学意义（P＞0.05）。光镜、电镜示针刺组泪腺上皮细胞胞浆丰富，排列紧密，活动良好，与空白组接近。免疫组织化学检测显示与模型组比较，针刺组泪腺导管及腺泡上皮细胞中Bax的阳性细胞半定量评分值明显低，差异有统计学意义（P＜0.05）；Bcl-2的阳性细胞半定量评分值亦低，但差异无统计学意义（P＞0.05）。与空白组相比，针刺组Bax、Bcl-2评分值差异均无统计学意义（均为P＞0.05）。与模型组比较，针刺组的泪腺导管及腺泡上皮细胞中Fas、FasL的阳性细胞半定量评分值明显低，差异均有统计学意义（均为P＜0.05）。与空白组相比，针刺组Fas、FasL评分值差异均无统计学意义 (均为P＞0.05)。结论　针刺通过抑制干眼兔泪腺组织的细胞凋亡及腺体萎缩，促进兔干眼模型的泪腺代谢，从而达到增加泪液分泌的作用。
Abstract:
Objective　To investigate the effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbit,and explore the possible mechanism of acupuncture on dry eye.Methods　Eighteen healthy New Zealand white rabbits were randomly divided into three groups:blank group,model group,acupuncture group,6 rabbits in each group．Blank group did not accept any treatment.Model group and acupuncture group were treated with subcutaneous injections of scopolamine hydrobromide,4 times a day with the dose of 2.0 mg·kg-1,when the acupuncture group was also treated with acupuncture,once a day for fourteen days.Three groups were examined Schirmer I test (SⅠt) at different stages,and morphological changes of lacrimal gland under light and electron microscope were observed.The immunohistochemical method was used to detect the protein levels of apoptosis related gene in lacrimal gland of all rabbits.Results　Between the acupuncture group and model group,the SⅠt was significantly different on day 4,7,14 (all P<0.05).At the same time,compared with that before experiment,SⅠt had no significant difference on day 14 in acupuncture group (P＞0.05).Optical and electron microscope showed the lacrimal gland epithelial cells of acupuncture group closely arranged with abundant cytoplasm,closed to that of the blank group.Meanwhile,the number of lacrimal epithelium cells with Bax,Fas and FasL positive expression was significantly less than that in the model group (all P<0.05).But when it compared with blank group,there was no significant difference of Bax/Bcl-2 and Fas/FasL positive expression in acupuncture group (all P>0.05).Conclusion　Acupuncture can restrain the apoptosis and gland atrophy of lacrimal gland tissue of dry eye rabbit,and promote metabolism,thereby increase the tear secretion.

参考文献/References:

［1］　赵堪兴,杨培增.眼科学［M］.北京：人民卫生出版社,2013:92.
ZHAO KX,YANG PZ.Ophthalmology［M］.Beijing:People’s Medical Publishing House,2013:92.
［2］　李忠仁.实验针灸学［M］.北京：中国中医药出版社,2003:319.
LI ZR.Experimental acupuncture science［M］.Beijing:China Press of Traditional Chinese Medicine,2003:319.
［3］　曲利娟,余英豪,谈景旺,曾玲.大肠癌组织中Fas/FasL系统、nm23表达与转移的关系［J］.临床与实验病理学杂志,2002,18(2):153-155.
QU LJ,YU YH,TAN JW,ZENG L.Relationship of the expression of Fas/FasL system,nm23 and metastasis in colorectal carcinomas［J］.J Clin Exp Pathol,2002,18(2):153-155.
［4］　徐雯,刘雪,高卫萍.润目灵颗粒对水液缺乏性干眼兔泪腺形态学及细胞凋亡的影响［J］.中药新药与临床药理,2015，26(4):425-430.
XU W,LIU X,GAO WP.Influence of runmuling granules on morphology and apoptosis-related factors of lacrimal gland in aqueous tear deficiency dry eye model rabbits［J］.Tradit Chin Drug Res Pharmacol,2015,26(4):425-430.
［5］　刘雪,徐雯,高卫萍.硫酸阿托品滴眼液滴眼和氢溴酸东莨菪碱皮下注射制作水液缺乏型干眼兔模型实验研究［J］.临床眼科杂志,2015,23(3):263-266.
LIU X,XU W,GAO WP.Research on the atropine sulfate eye drops and scopolamine hydrobromide subcutaneous injection production water lack of rabbit’s dry eye model［J］.J Clin Ophthalmol,2015,23(3):263-266.
［6］　仇晶晶,袁进,周世有,刘菲,陈家祺.皮下注射氢溴酸东莨菪碱建立兔干眼模型的实验研究［J］.眼科,2009,18(6):404-409.
QIU JJ,YUAN J,ZHOU SY,LIU F,CHEN JQ.An experimental of rabbit tear-deficient dry eye model by subcutaneous injection of scopolamine hydrobromide［J］.Ophthalmol CHN,2009,18(6):404-409.
［7］　张月梅,高卫萍.针刺对去势雄兔干眼模型泪液分泌及泪腺上皮细胞相关蛋白Fas/FasL表达的影响［J］.辽宁中医药大学学报,2012，14(8):248-250.
ZHANG YM,GAO WP.Effect of acupuncture to castrated rabbit dry eye model secretion of tears and related proteins of the lacrimal gland epithelial cells Fas/FasL expression［J］.J Liaoning Univ Tradit Chin Med,2012，14(8):248-250.
［8］　NAGATA S,GOLSTEIN P.The Fas death factor［J］.Science,1995,267(5203):1449-1456.
［9］　ROSS T,OLIVIER R,MONNEY L,RAGER M,CONUS S,FELLAY I,et al.Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c［J］.Nature,1998,391(6666):496-499.
［10］　GAO J,SCHWALB TA,ADDEO JV,CHOSN CR,STERN ME.The role of apoptosis in the pathogenesis of canine keratoconjunctivitis sicca:the effect of topical Cyclosporin A therapy.［J］.Cornea,1998,17(6):654-663.
［11］　王方,彭清华,姚小磊,吴权龙,李怀凤,陈佳文.密蒙花总黄酮对去势导致干眼症雄鼠泪腺Bax mRNA、Bcl-2 mRNA表达的影响［J］.眼科新进展,2010,30(3):201-206.
WANG F,PENG QH,YAO XL,WU QL,LI HF,CHEN JW.Effect of buddleja officinali flavone on expression of Bax mRNA and Bcl-2 mRNA in induced dry eye in castrated rats［J］.Rec Adv Ophthalmol,2010,30(3):201-206.
［12］　MANGANELLI P,FIETTA P.Apoptosis and Sjgren syndrome［J］.Semin Arthr Rheum,2003,33(1):49-65.

相似文献/References:

[1]姜洋 李莹 王忠海 罗岩 金玉梅.小牛血去蛋白提取物眼用凝胶对LASIK 术后角膜修复及干眼的疗效观察[J].眼科新进展,2012,32(7):000.
[2]叶芬 王春红 陈银 施宇华 黄振平.人工泪液对糖尿病干眼患者泪河的影响[J].眼科新进展,2012,32(12):000.
[3]张磊 何彦津.无眼征Graves病患者的眼表损害[J].眼科新进展,2012,32(12):000.
[4]皮百木 穆红梅 张婉婷 郑娜 李娟.老年干眼患者眼表改变及其与睑板腺功能障碍的相关性[J].眼科新进展,2013,33(6):000.
[5]张宏 马俊旗 安晓 叶向彧.围绝经期干眼患者眼表CXCR3表达与性激素的相关性分析[J].眼科新进展,2013,33(9):000.
[6]邵春燕 张沧霞 郑艳霞 魏宝丰.彩色多普勒血流显像在前部缺血性视神经病变针刺效应评估中的应用研究[J].眼科新进展,2008,28(9):000.
[7]叶芬 田农 施宇华 薛春燕 黄振平.糖尿病干眼患者的波前像差分析[J].眼科新进展,2012,32(4):000.
[8]魏瑞华 郝永娜 赵少贞.自身免疫相关性干眼动物模型的研究进展[J].眼科新进展,2012,32(7):000.
[9]魏瑞华 郝永娜 边领斋 李雪 粘红 赵少贞.Sjogren综合征相关性干眼发病机制的研究进展[J].眼科新进展,2013,33(3):000.
[10]邱伟强 李学民 王薇 刘子源 张志红.泪点栓塞和人工泪液对干眼患者视功能和泪膜稳定性的影响[J].眼科新进展,2013,33(10):000.
[11]沈天怡,刘俊伶,蔡雯婷,等.针刺治疗绝经后期干眼患者的疗效及其泪液蛋白质组学研究[J].眼科新进展,2018,38(10):959.[doi:10.13389/j.cnki.rao.2018.0227]
　SHEN Tian-Yi,LIU Jun-Ling,CAI Wen-Ting,et al.Proteomic analysis of tears following acupuncture treatment for menopausal dry eye based on two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry[J].Recent Advances in Ophthalmology,2018,38(3):959.[doi:10.13389/j.cnki.rao.2018.0227]
[12]王娇娇,高卫萍,丁宁,等.针刺对干眼的抗炎作用以及与JAK2/STAT3信号转导通路的关系[J].眼科新进展,2019,39(8):719.[doi:10.13389/j.cnki.rao.2019.0164]
　WANG Jiao-Jiao,GAO Wei-Ping,DING Ning,et al.Anti-inflammatory effects of acupuncture on dry eye and its relationship with the signal pathway of Janus kinase 2/signal transducer and activator of transcription3[J].Recent Advances in Ophthalmology,2019,39(3):719.[doi:10.13389/j.cnki.rao.2019.0164]
[13]丁宁,韦庆波,刘成勇,等.针刺对干眼兔角膜形态学及角膜组织NF-κB信号通路的影响[J].眼科新进展,2024,44(3):173.[doi:10.13389/j.cnki.rao.2024.0034]
　DING Ning,WEI Qingbo,LIU Chengyong,et al.Effect of acupuncture on corneal morphology and nuclear factor κB signaling pathway of dry eye rabbits[J].Recent Advances in Ophthalmology,2024,44(3):173.[doi:10.13389/j.cnki.rao.2024.0034]

备注/Memo

备注/Memo:
国家自然科学基金资助(编号：81373746)
更新日期/Last Update: 2017-03-30