[1]王业青,张晓梅,董丽,等.雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响[J].眼科新进展,2017,37(3):215-219.[doi:10.13389/j.cnki.rao.2017.0055]
　WANG Ye-Qing,ZHANG Xiao-Mei,DONG Li,et al.Effects of 17β-estradiol on stromal cell-derived factor-1 expression after retinal ischemia-reperfusion injury[J].Recent Advances in Ophthalmology,2017,37(3):215-219.[doi:10.13389/j.cnki.rao.2017.0055]
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雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年3期
页码:
215-219
栏目:
实验研究
出版日期:
2017-03-05

文章信息/Info

Title:
Effects of 17β-estradiol on stromal cell-derived factor-1 expression after retinal ischemia-reperfusion injury
作者:
王业青; 张晓梅; 董丽; 石焕琦; 毕文娇; 侯文文
150001　黑龙江省哈尔滨市，哈尔滨医科大学附属第一医院眼科
Author(s):
WANG Ye-Qing; ZHANG Xiao-Mei; DONG Li; SHI Huan-Qi; BI Wen-Jiao; HOU Wen-Wen
Department of Ophthalmology，the First Affiliated Hospital of Harbin Medical University，Harbin 150001，Heilongjiang Province，China
关键词:
视网膜; 缺血-再灌注损伤; 雌二醇; 基质细胞衍生因子-1
Keywords:
retina; ischemia-reperfusion; 17β-estradiol; stromal cell-derived factor-1
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2017.0055
文献标志码:
A
摘要:
目的　通过观察视网膜缺血-再灌注损伤后基质细胞衍生因子-1（stromal cell-derived factor-1，SDF-1）的表达情况，以及雌二醇对SDF-1的表达及调控作用，研究雌二醇对大鼠视网膜缺血-再灌注损伤的保护作用机制。方法　通过升高大鼠眼压的方法建立视网膜缺血-再灌注损伤模型，应用RT-PCR和Western blot方法检测视网膜缺血-再灌注损伤后各时间点（6 h、12 h、24 h）视网膜SDF-1表达情况；腹腔注射雌二醇后观察雌二醇对视网膜缺血-再灌注损伤后SDF-1表达的影响；并且应用雌激素受体拮抗剂ICI 182-780研究雌激素受体对雌二醇诱导视网膜缺血-再灌注损伤后的SDF-1的影响。结果　SDF-1 mRNA和蛋白在缺血-再灌注6 h组、缺血-再灌注12 h组和缺血-再灌注24 h组表达均增加，与正常对照组比较差异均有统计学意义（均为P<0.05），并且在缺血-再灌注12 h组SDF-1表达达高峰。雌二醇预处理对视网膜缺血-再灌注具有一定的保护作用；缺血-再灌注+雌二醇组SDF-1 mRNA和蛋白表达增加，与缺血-再灌注对照组及缺血-再灌注+溶剂对照组比较差异均有统计学意义（均为P<0.05）。与缺血-再灌注+雌二醇组相比较，缺血-再灌注+雌二醇+ICI 182-780组SDF-1 mRNA和蛋白表达明显降低，差异均有统计学意义（均为P<0.05）。结论　雌二醇对视网膜缺血-再灌注损伤的保护作用是通过雌激素受体介导的SDF-1 mRNA和蛋白表达增强实现的。
Abstract:
Objective　To examine the expression of stromal cell-derived factor-1 (SDF-1) in a rat model of retinal ischemia-reperfusion injury (RIRI),and investigate the protective effect of 17β-Estradiol (E2) on RIRI and explore the mechanism.Methods　The RIRI model was established in Sprague-Dawley rats by increasing the intraocular pressure.Relative expression levels of SDF-1 mRNA and protein in the retina at 6 hours,12 hours and 24 hours following reperfusion was determined by RT-PCR and Western blot,respectively.E2 was administered to investigate the effects of estrogen on SDF-1 expression,and the estrogen receptor antagonist ICI 182-780 was administered to investigate the effect of estrogen receptor on the expression of SDF-1.Results　SDF-1 expression in RIRI 6 hours group,12 hours group and 24 hours group was increased compared with normal control group (all P<0.05),with maximum expression at RIRI 12 hours group.As expected,pretreatment of RIRI rats with E2 had a protection on RIRI retina;SDF-1 expression was increased in RIRI+E2 group compared with IR control group and RIRI+vehicle group (all P<0.05).RIRI+E2+ICI 182-780 group could decrease SDF-1 expression compared with RIRI+E2 group(all P<0.05).Conclusion　E2 offers protection against RIRI by inducing an up-regulation in SDF-1 expression through activation of the estrogen receptor.

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备注/Memo

备注/Memo:
黑龙江省卫生计生委科研课题（编号：2014-299）；黑龙江省博士后科研启动金（编号：LBH-Q15101）
更新日期/Last Update: 2017-03-30