[1]林凤彬,谭少健,梁皓,等.去整合素echistatin对糖尿病兔后发性白内障形成中信号通路PI3-K/Akt和ERK1/2的影响[J].眼科新进展,2016,36(5):406-409.[doi:10.13389/j.cnki.rao.2016.0109]
　LIN Feng-Bin,TAN Shao-Jian,LIANG Hao,et al.Effects of disintegrin echistatin on PI3-K/Akt and ERK1/2 signaling pathways in posterior capsule opacification model of diabetic rabbit[J].Recent Advances in Ophthalmology,2016,36(5):406-409.[doi:10.13389/j.cnki.rao.2016.0109]
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去整合素echistatin对糖尿病兔后发性白内障形成中信号通路PI3-K/Akt和ERK1/2的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年5期
页码:
406-409
栏目:
实验研究
出版日期:
2016-05-05

文章信息/Info

Title:
Effects of disintegrin echistatin on PI3-K/Akt and ERK1/2 signaling pathways in posterior capsule opacification model of diabetic rabbit
作者:
林凤彬; 谭少健; 梁皓; 陈迎迎
530021　广西壮族自治区南宁市，广西医科大学第一附属医院眼科
Author(s):
LIN Feng-Bin ; TAN Shao-Jian ;  LIANG Hao ; CHEN Ying-Ying
Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021 , Guangxi Zhuang Autonomous Region , China
关键词:
去整合素; Akt; ERK1/2; 后发性白内障; 糖尿病; 兔
Keywords:
disintegrin ;  Akt ;  ERK1/2 ;  posterior capsular opacification ;  diabetes ;  rabbit
DOI:
10.13389/j.cnki.rao.2016.0109
文献标志码:
A
摘要:
目的　观察去整合素ｅｃｈｉｓｔａｔｉｎ对糖尿病兔后发性白内障形成中信号通路ＰＩ３-Ｋ／Ａｋｔ和ＥＲＫ１／２的影响，从分子水平上探讨ｅｃｈｉｓｔａｔｉｎ的作用。方法　建立糖尿病兔模型（ｎ＝２４），随机分组并行透明晶状体囊外摘出术，术毕前房分别注入０．２ｍＬ灭菌蒸馏水（对照组，ｎ＝１２）或０．２ｍＬ１０．０ｍｇ·Ｌ－１ｅｃｈｉｓｔａｔｉｎ溶液（ｅｃｈｉｓｔａｔｉｎ干预组，ｎ＝１２）。术后１０ｄ及６周（每个时间点６眼），裂隙灯显微镜观察两组术眼ＰＣＯ分级情况，同时摘取术眼应用ＲＴ-ＰＣＲ法检测后囊膜上信号因子Ａｋｔ和ＥＲＫ１／２ｍＲＮＡ表达情况。结果　术后１０ｄ对照组和ｅｃｈｉｓｔａｔｉｎ干预组ＰＣＯ分级比较差异无统计学意义（Ｐ＝０．０９３），但ｅｃｈｉｓｔａｔｉｎ干预组ＰＣＯ１级眼数少于对照组；术后６周ｅｃｈｉｓｔａｔｉｎ干预组ＰＣＯ级别明显低于对照组（Ｐ＝０．００６）。对照组和ｅｃｈｉｓｔａｔｉｎ干预组ＡｋｔｍＲＮＡ相对表达量术后１０ｄ分别为０．９８１７±０．３８０４、０．４８１７±０．１６６５，术后６周分别为０．６５１７±０．２０４７、０．４０１７±０．１５１３，ｅｃｈｉｓｔａｔｉｎ干预组均明显低于对照组（Ｐ＝０．０１５，０．０３７）。对照组和ｅｃｈｉｓｔａｔｉｎ干预组ＥＲＫ１／２ｍＲＮＡ相对表达量术后１０ｄ分别为０．９４８３±０．２２７５、０．６１００±０．２８０６，术后６周分别为０．９２１７±０．２９９４、０．４６５０±０．１８００，ｅｃｈｉｓｔａｔｉｎ干预组均明显低于对照组（Ｐ＝０．０４５，０．００９）。结论　去整合素ｅｃｈｉｓｔａｔｉｎ对糖尿病兔后发性白内障的发生和发展有一定的抑制作用，其发生的机制可能与抑制信号因子Ａｋｔ和ＥＲＫ１／２表达，进而阻断信号通路ＰＩ３-Ｋ／Ａｋｔ和ＥＲＫ１／２的转导有关。
Abstract:
Objective To investigate the effects of disintegrin echistatin on PBK/Akt and ERKI/2 signaling pathways in the posterior capsule opacification ( PCO) model of diabetic rabbit. Methods Rabbits were induced diabetic model ( n = 24 ) . then accepted extracapsular lens extraction , and injected 0. 2 mL distilled water ( control group ; n = 12) or 0. 2 mL 10. 0 mg . L -l echistatin ( echistatin-treated group ;n = 12) into the anterior chamber randomly and intraoperatively. At postoperativelo days and 6 weeks, PCO severity of two groups was evaluated with slit lamp microscope, and the posterior capsules( n = 6 for each time point) were extracted to analyze the level of Akt and ERKI/2 mRNA. Results At 10 days postoperatively, the number of grade I of PCO in echistatin-treated group was lower than the control group ,though there was no significant difference in PCO grades in the two groups (P = 0. 093) ; At 6 weeks postoperatively , PCO grades of the echistatin-treated group were significantly lower than those of the control group (P = 0. 006 ) . Akt mRNA expression in control group and echistatintreated group were 0. 981 7 + 0. 380 4 and 0. 481 7 + 0. 166 5 at postoperative 10 days , respectively ,0. 65 1 7 + 0. 204 7 and 0. 401 7 + 0. 151 3 at postoperative 6 weeks , respectively , the echistatin-treated group were lower than the control group (P = 0. 015 .0. 037) . ERKI/2 mRNA expression in control group and echistatin-treated group were 0. 948 3 + 0. 227 5 and 0. 610 0 +0. 280 6 at postoperative 10 days , respectively,0. 921 7 +0. 299 4 and 0. 465 0 + 0. 180 0 at postoperative 6 weeks , respectively , the echistatin-treated group were lower than the control group ( P = 0. 045 , 0. 009 ) . Conclusion Echistatin can inhibit diabetic rabbit PCO occurrence and development after extracapsular lens extraction , which may be related to down-regulate the expression of Akt and ERKI/2 , then inhibit the PI3-K/Akt and ERKI/2 signaling pathways.

相似文献/References:

[1]陈迎迎,谭少健,梁皓,等. 去整合素Echistatin对糖尿病兔后发性白内障模型中晶状体上皮细胞间质转分化及胶原合成的影响[J].眼科新进展,2014,34(4):306.[doi:10.13389/j.cnki.rao.2014.0083]
[2]钱光霞,谭少健,梁皓,等.去整合素Echistatin抑制糖尿病兔远期后发性白内障[J].眼科新进展,2014,34(6):506.[doi:10.13389/j.cnki.rao.2014.0139]
[3]陈迎迎,谭少健,梁皓钱,等. 糖尿病兔后囊膜混浊发生过程中血清及房水中AGE和IGF1的变化及去整合素echistatin对二者的影响[J].眼科新进展,2014,34(7):612.[doi:10.13389/j.cnki.rao.2014.0168]
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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：８１１６０１２０）
更新日期/Last Update: 2016-05-04