[1]崔欣,汤欣,史学锋.生后早期双眼形觉剥夺对大鼠视觉传导通路电生理学特性的影响[J].眼科新进展,2016,36(5):410-413.[doi:10.13389/j.cnki.rao.2016.0110]
　CUI Xin,TANG Xin,SHI Xue-Feng.Effects of early binocular form deprivation on electrophysiological characteristics of visual pathway in rats[J].Recent Advances in Ophthalmology,2016,36(5):410-413.[doi:10.13389/j.cnki.rao.2016.0110]
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生后早期双眼形觉剥夺对大鼠视觉传导通路电生理学特性的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年5期
页码:
410-413
栏目:
实验研究
出版日期:
2016-05-05

文章信息/Info

Title:
Effects of early binocular form deprivation on electrophysiological characteristics of visual pathway in rats
作者:
崔欣; 汤欣; 史学锋
300020　天津市，天津医科大学眼科临床学院天津市眼科医院天津市眼科学与视觉科学重点实验室天津市眼科研究所
Author(s):
CUI Xin ; TANG Xin ; SHI Xue-Feng
Clinical College of Ophthalmology of Tianjin Medical University, Tianjin Eye Hospital, Tianjin Key Laboratory of Ophthalmology and Vision Science,Tianjin Eye Institute,Tianjin 300020??China
关键词:
视皮层; 双眼形觉剥夺; 图形视觉诱发电位; 全细胞膜片钳技术; 固有膜特性
Keywords:
visual cortex ;  binocular form deprivation ;  pattern visual evoked potential;  whole-cell patch clamp technique ; intrinsic membrane properties
DOI:
10.13389/j.cnki.rao.2016.0110
文献标志码:
A
摘要:
目的　探讨生后早期双眼形觉剥夺对大鼠视觉传导通路电生理学特性的影响。方法　５０只８ｄ龄健康ＳＰＦ级Ｗｉｓｔａｒ大鼠随机分为两组：双眼形觉剥夺组与正常对照组。两组均在正常光照环境下饲养（１２ｈ白天／黑夜交替环境），于视觉发育关键期后期（生后３８～４４ｄ）利用眼电生理诊断系统观察比较两组大鼠图形视觉诱发电位（ｐａｔｔｅｒｎｖｉｓｕａｌｅ-ｖｏｋｅｄｐｏｔｅｎｔｉａｌ，ＰＶＥＰ）中Ｐ１００波潜时及振幅的差异，利用全细胞膜片钳技术记录两组大鼠视皮层单个神经元固有膜特性值的差异。结果　双眼形觉剥夺组ＰＶＥＰ中Ｐ１００波的潜时值为（９５．６７±１４．６７）ｍｓ，与正常对照组的（８３．６６±１０．８２）ｍｓ相比明显延长（Ｐ＝０．００２）；双眼形觉剥夺组ＰＶＥＰ中Ｐ１００的振幅为（３．０２±１．０２）μＶ，明显低于正常对照组的（７．４２±１．６５）μＶ，差异有统计学意义（Ｐ＝０．０００）。双眼形觉剥夺组与正常对照组视皮层神经元细胞的膜电容分别为（５０．０１±１５．８６）ｐＦ和（４５．１８±１９．０９）ｐＦ，膜电阻分别为（５８．８５±１４．１５）ＭΩ和（５５．８４±１２．０３）ＭΩ，时间常数值分别为（４３．６０±１２．０９）ｍｓ和（３７．５２±１０．８４）ｍｓ，两组相比差异均无统计学意义（均为Ｐ＞０．０５）。结论　生后早期双眼形觉刺激不足对视觉传导通路信号传递有延迟作用，但对视皮层单个神经元细胞的固有电生理学特性无影响。
Abstract:
Objective To investigate the effects of binocular form deprivation on the electrophysiological characteristics of the visual pathway of postnatal rats. Methods Fifty Wistar rats of 8 postnatal days were randomly divided into binocular form deprivation group and normal control group. In this two groups , all of rats were housed in the same condition with reversed 12 hours/12 hours light/dark cycles. The latency and amplitude of PlOO-wave in these two groups were recorded by pattern visual evoked potential technique in postnatal 38 - 44 days when visual development was at the end of the critical period. Meanwhile .intrinsic membrane characteristics of single visual cortical neuron of rats in these two groups were also recorded through whole-cell patch clamp technique for comparative analysis. Results The latency of PlOO-were in binocular form deprivation group ( 95. 67 + 14. 67 ) ms was longer than that in normal control group ( 83. 66 + 10. 82 ) ms significantly (P = 0. 002 ) , while the peak amplitude of P100-were in binocular form deprivation group ( 3. 02 + 1. 02) VV was much lower than that in normal control group ( 7. 42 + 1. 65 ) vV ( P = 0. 000 ) . The membrane capacitance of visual cortical neurons in binocular form deprivation group and normal control group were ( 50. 01 + 15. 86) pF and ( 45 . 18 + 19. 09 ) pF, respectively , the membrane resistance were ( 58. 85 + 14. 15 ) M(1 and ( 55. 84 + 12. 03 ) Mfl , respectively , the time constant were ( 43. 60 + 12. 09 ) ms and ( 37. 52 + 10. 84 ) ms , respectively , there was no statistical difference between two groups ( all P > 0. 05 ) . Conclusion The postnatal deficiency of binocular form leads to delayed signal induction of visual pathway, but it has no effect on the intrinsic electrophysiological characteristics of visual cortical neurons.

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备注/Memo

备注/Memo:
国家自然科学基金项目（编号：８１３７１０４９）；天津市自然科学基金项目（编号：１１ＪＣＹＢＪＣ-１２７００）
更新日期/Last Update: 2016-05-04