[1]彭红娟,黎宗汉,郑彪,等.兔眼小梁切除术后滤过泡组织Fas mRNA的表达[J].眼科新进展,2016,36(2):118-120.[doi:10.13389/j.cnki.rao.2016.0032]
　PENG Hong-Juan,LI Zong-Han,ZHENG Biao,et al.Expression of Fas mRNA of filtration bleb after trabeculectomy in rabbit[J].Recent Advances in Ophthalmology,2016,36(2):118-120.[doi:10.13389/j.cnki.rao.2016.0032]
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兔眼小梁切除术后滤过泡组织Fas mRNA的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年2期
页码:
118-120
栏目:
实验研究
出版日期:
2016-02-05

文章信息/Info

Title:
Expression of Fas mRNA of filtration bleb after trabeculectomy in rabbit
作者:
彭红娟; 黎宗汉; 郑彪; 方林彬; 柯毅
524001　广东省湛江市，广东医学院附属医院眼科
Author(s):
PENG Hong-Juan ;  LI Zong-Han; ZHENG Biao ;  FANG Lin-Bin ;  KE Yi
Department of Ophthalmology,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001, Guangdong Province.China
关键词:
青光眼; 小梁切除术; 滤过泡; Fas mRNA; 兔
Keywords:
glaucoma ;  trabeculectomy ;  filtration bleb ;  Fas mRNA ;  rabbit
DOI:
10.13389/j.cnki.rao.2016.0032
文献标志码:
A
摘要:
目的　对小梁切除术后滤过泡组织ＦａｓｍＲＮＡ的表达进行初步研究。方法　新西兰白兔４８只单独笼养，随机选取４０只兔右眼行小梁切除术，使上方结膜形成滤过泡，按术后时间又分为１ｄ、７ｄ、１４ｄ、２０ｄ、２８ｄ共５个亚组，每组８只兔。另外８只兔的右眼未进行手术为正常对照组。在行小梁切除术后１ｄ、７ｄ、１４ｄ、２０ｄ、２８ｄ，切下滤过手术区组织，同时于２８ｄ时切除正常对照组相应部位的结膜组织。实时荧光定量ＰＣＲ检测各组标本的ＦａｓｍＲＮＡ表达情况。结果　兔眼小梁切除术后１ｄ、７ｄ、１４ｄ三个亚组ＦａｓｍＲＮＡ的相对表达量分别为１．３３±０．３５、１．４１±０．２８、１．３８±０．２５，与正常对照组的表达量１．４２±０．３４相比差异无统计学意义（Ｐ＞０．０５），术后２０ｄＦａｓｍＲＮＡ相对表达量为０．９０±０．１８，较对照组明显下降，差异有统计学意义（Ｐ＜０．０５），２８ｄ时进一步下降至０．７８±０．０９（Ｐ＜０．０５）。结论　在兔眼结膜滤过泡组织愈合过程中，术后２０ｄ及２８ｄ凋亡主要基因ＦａｓｍＲＮＡ表达量明显减少，可能为减少青光眼小梁切除术后瘢痕化寻找到一个新的突破口。
Abstract:
Objective To study the expression of Fas mRNA in filtration bleb after trabeculectomy in rabbits. Methods Forty-eight New Zealand rabbits were fed singly,in which 40 cases were randomly chosen to perform the trabeculectomy in the right eye , then the rabbits were sub-divided int0 1-day group ,7-day group , 14-day group , 20-day group and 28-day group based on the postoperative time ,8 cases in each group. Another 8 rabbits received no any operation as the normal control group. At I day,7 days ,14 days ,20 days ,28 days after trabeculectomy , the filtration bleb tissues of operated groups were harvested respectively. The conjunctiva tissues of control group were also harvested at 28 days. The expression level of Fas mRNA was detected by Real Time RT-PCR. Results The relative expression of Fas mRNA at I day ,7 days,14 days after operation were l. 33 + 0. 35 . 1. 41 + 0. 28 . 1. 38 + 0. 25 , respectively , compared with the normal control group ( 1. 42 + 0. 34 ) the difference was not significant (P > 0. 05 ) . The relative expression of Fas mRNA at 20 days after operation was 0. 90 +0. 18 ,which was lower than that in the normal control group ( P < 0. 05 ) . and decreased t0 0. 78 + 0. 09 at 28 days after operation. Conclusion In the procession of filtering bleb tissue healing, the expression of Fas mRNA decrease at 20 days and 28 days,14 days after operation, which may be emerged as a promising target for treating postoperative scar of trabeculectomy.

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备注/Memo

备注/Memo:
广东省湛江市科技计划项目（编号：２０１２０６０２）
更新日期/Last Update: 2016-01-29