[1]张海江,邢怡桥,梁亮,等.视网膜缺血再灌注损伤大鼠视网膜中白细胞介素-23和白细胞介素-17的表达及意义[J].眼科新进展,2016,36(2):121-125.[doi:10.13389/j.cnki.rao.2016.0033]
　ZHANG Hai-Jiang,XING Yi-Qiao,LIANG Liang,et al.Expression and significance of interleukin-23 and interleukin-17 in rat retina after ischemia-reperfusion injury[J].Recent Advances in Ophthalmology,2016,36(2):121-125.[doi:10.13389/j.cnki.rao.2016.0033]
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视网膜缺血再灌注损伤大鼠视网膜中白细胞介素-23和白细胞介素-17的表达及意义
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年2期
页码:
121-125
栏目:
实验研究
出版日期:
2016-02-05

文章信息/Info

Title:
Expression and significance of interleukin-23 and interleukin-17 in rat retina after ischemia-reperfusion injury
作者:
张海江; 邢怡桥; 梁亮; 金玮; 李岱
443003　湖北省宜昌市，三峡大学第一临床医学院，三峡大学眼科与视觉科学研究所，宜昌市中心人民医院眼科
Author(s):
ZHANG Hai-Jiang ; XING Yi-Qiao ;  LIANG Liang ; JIN Wei;  LI Dai
First College of Clinical Medicine Science ,Institute of Ophthalmology and Visual Science of Three Gorges University , Department of Ophthalmology , Yichang Central People ’ s Hospital , Yichang 443003 , Hubei Province , China
关键词:
视网膜缺血再灌注损伤; 白细胞介素-23; 白细胞介素-17
Keywords:
retinal ischemia-reperfusion injury ; interleukin-23 ;  interleulan-17
DOI:
10.13389/j.cnki.rao.2016.0033
文献标志码:
A
摘要:
目的　通过建立视网膜缺血再灌注损伤（ｒｅｔｉｎａｌｉｓｃｈｅｍｉａ-ｒｅｐｅｒｆｕｓｉｏｎｉｎｊｕｒｙ，ＲＩＲＩ）模型，观察白细胞介素-２３（ｉｎｔｅｒｌｅｕｋｉｎ-２３，ＩＬ-２３）和白细胞介素-１７（ｉｎｔｅｒｌｅｕｋｉｎ-１７，ＩＬ-１７）在Ｓｐｒａｇｕｅ-Ｄａｗｌｅｙ（ＳＤ）大鼠视网膜上的表达，探讨其在ＲＩＲＩ中的可能作用机制。方法　采用随机数字表法将７５只健康无眼疾ＳＤ大鼠随机分为正常对照组和模型组。正常对照组不作任何处理，模型组采用前房灌注生理盐水升高眼压法建立ＲＩＲＩ模型。分别在建模后１２ｈ、２４ｈ、７２ｈ、１４４ｈ处死ＳＤ大鼠，免疫组织化学染色法检测ＩＬ-２３和ＩＬ-１７蛋白在视网膜上的表达及分布情况；Ｗｅｓｔｅｒｎｂｌｏｔ和ＥＬＩＳＡ检测分析各时间点视网膜ＩＬ-２３和ＩＬ-１７的表达水平和变化规律。结果　免疫组织化学染色法显示ＩＬ-２３和ＩＬ-１７蛋白在正常对照组视网膜中表达极少，而在模型组表达明显增多。两者主要表达于视网膜神经节细胞层和内核层细胞的细胞浆。Ｗｅｓｔｅｒｎｂｌｏｔ法检测发现ＩＬ-２３和ＩＬ-１７蛋白在ＲＩＲＩ视网膜中的表达明显增强，ＩＬ-２３蛋白在建模后２４ｈ表达最强，而ＩＬ-１７蛋白在建模后７２ｈ表达达到最高峰。ＥＬＩＳＡ法结果显示建模后１２ｈ、２４ｈ、７２ｈ、１４４ｈ，模型组视网膜ＩＬ-２３和ＩＬ-１７蛋白的表达水平均较正常对照组明显升高，差异均有显著统计学意义（均为Ｐ＜０．０１）。结论　ＩＬ-２３和ＩＬ-１７在ＲＩＲＩ模型ＳＤ大鼠视网膜中表达明显增强，ＩＬ-２３／ＩＬ-１７通路作为致病因素，通过加重视网膜炎症反应参与ＲＩＲＩ的病理损伤。
Abstract:
Objective To observe the expression of interleukin-23 ( IL-23) and interleukin-17 ( IL-17 )in rat retina after ischemia-reperfusion injury , and explore their role in retinal ischemia-reperfusion injury ( RIRI) . Methods A total of 75 Sprague-Dawley rats were divided into normal control group ( NCG) and model group ( MG) . The rats in NCG group were not treated. The rats in MG group were induced the RIRI models with normal saline by anterior chamber perfusion. Immunohistochemistry staining was used to detect specific lesions expression of IL-23 and IL-17 at 12 hours ,24 hours ,72 hours. 144 hours after RIRI. Both levels of IL-23 and IL-17 protein and changes were quantified by Western-blot and Enzyme-Iinked immunosorbent assay( ELISA) . Results Immunohistochemistry staining results showed that IL-23 and IL-17 expressions were very few in NCG group, but were rncreased significantly in MG group. The expression of positive signal mainly located in ganglion cell layer and inner nuclear layer of retina. Westernblot test analysis revealed that IL-23 and IL-17 expressions were increased significantly after RIRI , IL-23 was the highest at 24 hours .while IL-17 was the highest at 72 hours respectively after RIRI. ELISA assay showed that the expression of IL-23 , IL-17 in MG group were increased at 12 hours . 24 hours , 72 hours , 144 hours after RIRI, and had a significant statistical difference compared to NCG group ( all P < 0. 01 ) . Conclusion The expression levels of IL-23 and IL-17 are increased significantly in retina after RIRI. which maybe suggests that both aggravate inflammatory response and participate in RIRI , leading to retinal damage.

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更新日期/Last Update: 2016-01-29