[1]石璐,汪昌运.原花青素对氧化应激人眼小梁网细胞凋亡及细胞色素C释放的影响[J].眼科新进展,2017,37(10):931-934.[doi:10.13389/j.cnki.rao.2017.0236]
　SHI Lu,WANG Chang-Yun.Effects of procyanidins on cell apoptosis and the release of cytochrome C in human trabecular meshwork cells under oxidative stress[J].Recent Advances in Ophthalmology,2017,37(10):931-934.[doi:10.13389/j.cnki.rao.2017.0236]
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原花青素对氧化应激人眼小梁网细胞凋亡及细胞色素C释放的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年10期
页码:
931-934
栏目:
实验研究
出版日期:
2017-10-05

文章信息/Info

Title:
Effects of procyanidins on cell apoptosis and the release of cytochrome C in human trabecular meshwork cells under oxidative stress
作者:
石璐; 汪昌运
330006　江西省南昌市，南昌大学第二附属医院眼科
Author(s):
SHI Lu; WANG Chang-Yun
Department of Ophthalmology，the Second Affiliated Hospital，Nanchang University，Nanchang 330006，Jiangxi Province，China
关键词:
原花青素; 小梁网细胞; 氧化应激; 细胞凋亡率; 细胞色素C
Keywords:
procyanidins; trabecular meshwork cells; oxidative stress; cell apoptosis rate; cytochrome C
分类号:
R775
DOI:
10.13389/j.cnki.rao.2017.0236
文献标志码:
A
摘要:
目的　研究原花青素对H2O2诱导人眼小梁网细胞 （human trabecular meshwork cells，HTMC）凋亡及细胞色素C释放的影响。方法　将HTMC进行传代后随机分为5组。未处理组：正常培养的HTMC；对照组：正常培养的HTMC+ H2O2 （500 μmol·L-1 处理1 h）；3个浓度的原花青素组：正常培养的HTMC+ H2O2 （500 μmol·L-1 处理1 h）+原花青素（浓度分别为0.02 g·L-1、0.05 g·L-1、0.10 g·L-1）。使用Annexin V-FITC检测细胞凋亡情况，Western blot检测HTMC胞浆细胞色素C含量。结果　与未处理组相比，对照组及各原花青素组细胞凋亡率均升高，差异均有统计学意义（均为P＜0.01）。与对照组相比，各原花青素组细胞凋亡率均降低，差异均有统计学意义（均为P＜0.01）；不同浓度原花青素组间随原花青素浓度增加细胞凋亡率下降，各原花青素组间差异均有统计学意义（均为P＜0.01）。与未处理组相比，对照组以及0.02 g·L-1和0.05 g·L-1 原花青素组细胞色素C的释放均增加，差异均有统计学意义（均为P＜0.01）；而0.10 g·L-1 原花青素组与未处理组相比，差异无统计学意义（P>0.05）。与对照组相比，各原花青素组细胞色素C释放均减少，差异均有统计学意义（均为P＜0.01）。不同浓度原花青素组间随原花青素浓度增加细胞色素C的释放减少，各组间差异均有统计学意义（均为P＜0.01）。结论　外源性原花青素可以降低氧化应激HTMC的凋亡率，减少细胞色素C的释放，有较强的抗氧化作用，并在一定浓度范围内随着浓度的增加其抗氧化作用逐渐增强。
Abstract:
Objective　To investigate the effect of procyanidins (PC) on cell apoptosis and cytochrome C releasing in human trabecular meshwork cells (HTMC) under oxidative stress induced by H2O2.Methods　HTMC were cultured and then divided randomly into 5 groups.Normal cultured HTMC served as normal group without treatment，and normal cultured HTMC in control group were treated with 500 μmol·L-1 H2O2 for 1 h，as well as normal cells in treatment groups were treated with 500 μmol·L-1 H2O2 for 1 h combined with different concentrations of PC (0.02 g·L-1，0.05 g·L-1，0.10 g·L-1).Then flow cytometry and Western blot were applied to detect the cell apoptosis rate and the release of cytochrome C (CytC) respectively.Results　Compared with the normal group，the apoptotic rates of the control group and the PC treatment groups were significantly increased，and the difference was statistically significant (all P<0.01).Compared with the control group，the apoptotic rates of the 3 PC treatment groups were decreased，and the differences were statistically significant (all P<0.01).Moreover，the apoptotic rate in 0.02 g·L-1，0.05 g·L-1，0.10 g·L-1 PC treatment groups was decreased as PC concentration increased，and the differences were statistically significant (all P<0.01).Furthermore，compared with the normal group，the release of cytochrome C in the control group，0.02 g·L-1 and 0.05 g·L-1 PC treatment group enhanced，and the differences were statistically significant (all P<0.01)，but there was no significant difference between 0.01 g·L-1 PC treatment group and the normal group (P>0.05).Compared with the control group，the release of cytochrome C in the 3 PC treatment group was attenuated with significant differences (all P<0.01).Meanwhile，the release of cytochrome C was decreased in the 3 PC treatment groups with a concentration-dependent manner，and the pairwise differences were statistically significant (all P<0.01).Conclusion　Exogenous PC can decrease the apoptotic rate of HTMC under oxidative stress and reduce the release of cytochrome C，as well as it also has an antioxidant effect in a certain concentration-dependent manner.

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更新日期/Last Update: 2017-10-24