[1]石璐,汪昌运.原花青素对H2O2诱导人小梁细胞氧化应激的抗氧化作用[J].眼科新进展,2017,37(2):121-124.[doi:10.13389/j.cnki.rao.2017.0032]
　SHI Lu,WANG Chang-Yun.Protective effects of procyanidins on human trabecular meshwork cells against H2O2 induced oxidative stress [J].Recent Advances in Ophthalmology,2017,37(2):121-124.[doi:10.13389/j.cnki.rao.2017.0032]
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原花青素对H2O2诱导人小梁细胞氧化应激的抗氧化作用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年2期
页码:
121-124
栏目:
实验研究
出版日期:
2017-02-05

文章信息/Info

Title:
Protective effects of procyanidins on human trabecular meshwork cells against H2O2 induced oxidative stress
作者:
石璐; 汪昌运
330006　江西省南昌市，南昌大学第二附属医院眼科
Author(s):
SHI Lu; WANG Chang-Yun
Department of Ophthalmology，the Second Affiliated Hospital，Nanchang University，Nanchang 330006，Jiangxi Province，China
关键词:
原花青素; 小梁细胞; 氧化应激; 青光眼
Keywords:
procyanidins; trabecular meshwork cells; oxidative stress; glaucoma
分类号:
R775
DOI:
10.13389/j.cnki.rao.2017.0032
文献标志码:
A
摘要:
目的　研究原花青素（Procyanidins，PC）对H2O2诱导人眼小梁细胞(human trabecular meshwork cells，HTMC)氧化应激的抗氧化作用，为青光眼的临床治疗提供实验依据。方法　将正常HTMC进行细胞传代后随机分为5组。未处理组：正常培养的HTMC；对照组：正常培养的HTMC+H2O2（500 μmol·L-1处理1 h）；PC组：正常培养的HTMC+H2O2（500 μmol·L-1处理1 h）+PC（浓度分别为0.02 g·L-1、0.05 g·L-1、0.10 g·L-1）。应用实时荧光定量PCR方法检测线粒体复合物I mRNA的表达。结果　与未处理组(1.000 0±0.000 0)相比，0.02 g·L-1 PC组(0.401 3±0.010 3)和0.05 g·L-1 PC组(0.791 5±0.008 5)线粒体复合物I mRNA表达差异有统计学意义（均为P<0.01），而0.10 g·L-1 PC组(1.043 0±0.062 2)差异无统计学意义（P>0.05）；与对照组(0.095 0±0.006 5)相比，各PC处理组线粒体复合物I mRNA表达均增加，差异均有统计学意义（均为P<0.01）；不同浓度PC组间随PC浓度增加线粒体复合物I mRNA表达增加，各PC浓度组组间差异有统计学意义（P<0.01）。结论　外源性PC可以增加氧化应激HTMC线粒体复合物I mRNA的表达，有较强的抗氧化作用，并在一定范围内随着浓度的增加其抗氧化作用逐渐增强，PC在青光眼的临床治疗中的作用值得进一步研究。
Abstract:
Objective　To investigate the protection and mechanism of procyanidins (PC) against H2O2 induced oxidative damage of human trabecular meshwork cells (HTMC) in order to provide an experimental foundation for glaucoma clinical treatment.Methods　HTMC were cultured and then divided randomly into 5 groups.As untreated group:Normal cultured HTMC;Control group:Normal cultured HTMC+ H2O2 (500 μmol·L-1 for 1 hour);Treated group:Normal cultured HTMC+H2O2 (500 μmol·L-1 for 1 hour) + PC (PC final concentrations were 0.02 g·L-1,0.05 g·L-1,0.10 g·L-1).Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to investigate the expression of mitochondrial complex I mRNA.Results　Compared with untreated group (1.000 0±0.000 0),the differences of mitochondrial complex I mRNA expression in 0.02 g·L-1 PC (0.401 3±0.010 3),0.05 g·L-1 PC (0.791 5±0.008 5) groups were statistically significant (all P<0.01),but the 0.10 g·L-1 PC group (1.043 0±0.062 2) had no significant differences (P>0.05).The differences between PC treated groups and control group were statistically significant (P<0.01),which showed HTMC treated with PC could increase the expression of mitochondrial complex I mRNA.The differences in each PC treated groups were statistically significant (P<0.01),which showed the expression of mitochondrial complex I mRNA were increased along with the concentration of PC gradually increased.Conclusion　Exogenetic PC can increase the expression of mitochondrial complex I mRNA in the oxidative damaged HTMC,and in a certain range of concentration,the protective effects of PC have the positive relationship of dose-effect,which suggest that PC may be a good candidate for further study of the clinical treatment of glaucoma.

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更新日期/Last Update: 2017-03-01