[1]樊倩,王雁,段学欣,等.器官芯片在眼科领域的研究进展[J].眼科新进展,2021,41(10):978-981.[doi:10.13389/j.cnki.rao.2021.0206]
　FAN Qian,WANG Yan,DUAN Xuexin,et al.Research advances on application of organs-on-chips in ophthalmology[J].Recent Advances in Ophthalmology,2021,41(10):978-981.[doi:10.13389/j.cnki.rao.2021.0206]
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
41卷
期数:
2021年10期
页码:
978-981
栏目:
文献综述
出版日期:
2021-10-05

文章信息/Info

Title:
Research advances on application of organs-on-chips in ophthalmology
作者:
樊倩; 王雁; 段学欣; 薛茜男; 游睿; 杨文拓; 杨洋
300020　天津市，天津市眼科医院，天津市眼科学与视觉科学重点实验室，南开大学附属眼科医院，天津医科大学眼科临床学院，天津市眼科研究所（樊倩，王雁）；300072　天津市，天津大学精密仪器与光电子工程学院，天津大学精密测量技术与仪器国家重点实验室（段学欣，薛茜男，游睿，杨文拓，杨洋）
Author(s):
FAN Qian1; WANG Yan1; DUAN Xuexin2; XUE Qiannan2; YOU Rui2; YANG Wentuo2; YANG Yang2
1.Tianjin Eye Hospital,Tianjin Key Lab of Ophthalmology and Visual Science,Nankai University Affiliated Eye Hospital,Clinical College of Ophthalmology,Tianjin Medical University,Tianjin Eye Institute,Tianjin 300020,China
2.State Key Laboratory of Precision Measuring Technology and Instruments,College of Precision Instrument and Opto-electronics Engineering,Tianjin University,Tianjin 300072,China
关键词:
器官芯片; 眼科; 微流控技术; 微生理系统
Keywords:
organs-on-chips;  ophthalmology;  microfluidics;  microphysiological systems
分类号:
R77
DOI:
10.13389/j.cnki.rao.2021.0206
文献标志码:
A
摘要:
器官芯片是生命科学领域的新兴前沿科学，它利用微流控技术构建以模拟人体组织和器官功能为目标的集成微系统，可以为药物筛选和疫苗的有效性、安全性评估以及其他多种生物医学研究提供更接近人体真实生理和病理条件的低成本研究模型。器官芯片不仅可以模拟人体目标器官的三维微环境，而且具备样品消耗少、检测速度快、操作简便、多功能集成、精度高、自动化和便携等优点，拥有简化采集标本及诊断流程，助力提高医疗效果的巨大潜力。目前，微流控器官芯片技术正以惊人的速度快速发展，心脏、肾脏、肝脏、肺脏以及血管等全身多个器官组织相关芯片产品陆续研发成功。眼科领域的器官芯片研究目前集中在角膜相关疾病、视网膜及脉络膜疾病等。本文就器官芯片在眼科领域中的研究进展进行综述。
Abstract:
Organs-on-chips (OOCs) technology is one of the cutting-edge research hotspots. Using the microfluidic chip system, an integrated microsystem is constructed to simulate the functions of human tissues and organs, which can be used for drug screening, vaccine effectiveness and biological safety evaluation and an unprecedented cheap and convenient platform establishment. OOCs technology can not only simulate the three-dimensional microenvironment of human target organs, but also show great potential in simplifying the sample collection and diagnosis process to improve the medical effect based on the characteristics of small sample size, fast detection speed, convenient operation, high precision, multi-function and automation. At present, OOCs technology is developing at an astonishing speed. In many organs and tissues such as heart, kidney, liver, lung and blood vessels, the related OOCs products have been successfully developed. The research of OOCs in ophthalmology focuses on corneal, retinal and choroidal diseases. This paper reviews the research progress of OOCs in the field of ophthalmology.

参考文献/References:

［1］　VIRAVAIDYA K,SIN A,SHULER M L.Development of a microscale cell culture analog to probe naphthalene toxicity［J］.Biotechnol Prog,2004,20(1):316-323.
［2］　VIRAVAIDYA K,SHULER M L.Incorporation of 3T3-L1 cells to mimic bioaccumulation in a microscale cell culture analog device for toxicity studies［J］.Biotechnol Prog,2004,20(2):590-597.
［3］　HUH D,MATTHEWS B D,MAMMOTO A,MONTOYA-ZAVALA M,HSIN H Y,INGBER D E.Reconstituting organ-level lung functions on a chip［J］.Science,2010,328(5986):1662-1668.
［4］　BHATIA S N,INGBER D.Microfluidic organs-on-chips［J］.Nat Biotechnol,2014,32(8):760-772.
［5］　SKARDAL A,SHUPE T,ATALA A.Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling［J］.Drug Discov Today,2016,21(9):1399-1411.
［6］　NAWROTH J C,BARRILE R,CONEGLIANO D,VAN RIET S,HIEMSTRA P S,VILLENAVE R.Stem cell-based lung-on-chips:the best of both worlds?［J］.Adv Drug Deliv Rev,2019,140:12-32.
［7］　COCHRANE A,ALBERS H J,PASSIER R,MUMMERY C L,VAN DEN BERG A,ORLOVA V V,et al.Advanced in vitro models of vascular biology:human induced pluripotent stem cells and organ-on-chip technology［J］.Adv Drug Deliv Rev,2019,140:68-77.
［8］　WANG J,KHODABUKUS A,RAO L,VANDUSEN K,ABUTALEB N,BURSAC N.Engineered skeletal muscles for disease modeling and drug discovery［J］.Biomaterials,2019,221:119416.
［9］　NANGIA V,JONAS J B,GEORGE R,LINGAM V,ELLWEIN L,CICINELLI M V,et al.Prevalence and causes of blindness and vision impairment:magnitude,temporal trends and projections in South and Central Asia［J］.Br J Ophthalmol,2019,103(7):871-877.
［10］　BOURNE R A,FLAXMAN S R,BRAITHWAITE T,CICINELLI M V,DAS A,JONAS J B,et al.Magnitude,temporal trends,and projections of the global prevalence of blindness and distance and near vision impairment:a systematic review and meta-analysis［J］.Lancet Glob Heal,2017,5(9):e888-e897.
［11］　PASCOLINI D,MARIOTTI S P.Global estimates of visual impairment:2010［J］.Br J Ophthalmol,2012,96(5):614-618.
［12］　ZHANG J,TUO J,WANG Z,ZHU A,MACHALI N ＇ SKA A,LONG Q.Pathogenesis of common ocular diseases［J］.J Ophthalmol,2015,2015:734527.
［13］　BENNET D,ESTLACK Z,REID T,KIM J.A microengineered human corneal epithelium-on-a-chip for eye drops mass transport evaluation［J］.Lab Chip,2018,18(11):1539-1551.
［14］　SEO J,BYUN W Y,ALISAFAEI F,GEORGESCU A,YI Y S,MASSARO-GIORDANO M,et al.Multiscale reverse engineering of the human ocular surface［J］.Nat Med,2019,25(8):1310-1318.
［15］　ABDALKADER R,KAMEI K I.Multi-corneal barrier-on-a-chip to recapitulate eye blinking shear stress forces［J］.Lab Chip,2020,20(8):1410-1417.
［16］　弥胜利,赵港南,杨星.一种功能性微流控角膜接触镜及其制备方法:CN109288619A［P］.2019-02-01.
MI S L,ZHAO G N,YANG X.A functional microfluidic contact lens and its preparation method:CN109288619A［P］.2019-02-01.
［17］　CHEN L J,ITO S,KAI H,NAGAMINE K,NAGAI N,NISHIZAWA M,et al.Microfluidic co-cultures of retinal pigment epithelial cells and vascular endothelial cells to investigate choroidal angiogenesis［J］.Sci Rep,2017,7(1):3538.
［18］　YESTE J,GARCA-RAMREZ M,ILLA X,GUIMER A,HERNNDEZ C,SIM R,et al.A compartmentalized microfluidic chip with crisscross microgrooves and electrophysiological electrodes for modeling the blood-retinal barrier［J］.Lab Chip,2017,17(1):95-105.
［19］　SU P J,LIU Z,ZHANG K,HAN X,SAITO Y,XIA X,et al.Retinal synaptic regeneration via microfluidic guiding channels［J］.Sci Rep,2015,5:13591.
［20］　CHUNG M,LEE S,LEE B J,SON K,JEON N L,KIM J H.Wet-AMD on a chip:modeling outer blood-retinal barrier in vitro［J］.Adv Healthc Mater,2018,7(2):201700028.

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备注/Memo

备注/Memo:
国家自然科学基金面上项目（编号：81873684）；天津市卫生计生委科技基金重点项目（编号：2014KR17）；天津市眼科医院科技基金面上项目（编号：YKYB2007）
更新日期/Last Update: 2021-10-05