[1]纪艳丽,彭广华.单细胞转录组测序在眼科研究领域中的应用[J].眼科新进展,2018,38(1):084-88.[doi:10.13389/j.cnki.rao.2018.0019]
　JI Yan-Li,PENG Guang-Hua.Application of single-cell RNA-sequencing in ophthalmology[J].Recent Advances in Ophthalmology,2018,38(1):084-88.[doi:10.13389/j.cnki.rao.2018.0019]
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单细胞转录组测序在眼科研究领域中的应用/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年1期
页码:
084-88
栏目:
文献综述
出版日期:
2018-01-05

文章信息/Info

Title:
Application of single-cell RNA-sequencing in ophthalmology
作者:
纪艳丽; 彭广华
450052　河南省郑州市，郑州大学医学科学院，病理学与病理生理学系
Author(s):
JI Yan-Li; PENG Guang-Hua
Department of Pathology and Pathophysiology，the Academy of Medical Sciences，Zhengzhou University，Zhengzhou 450052，Henan Province，China
关键词:
单细胞; RNA测序; 转录组; 异质性; 眼科
Keywords:
single cell; RNA-sequencing; transcriptome; heterogeneity; ophthelmology
分类号:
R77
DOI:
10.13389/j.cnki.rao.2018.0019
文献标志码:
A
摘要:
单细胞转录组测序技术（single-cell RNA-sequencing，sc RNA-seq）是从单个细胞水平分析细胞转录组的表达谱，用于鉴定细胞特异性标记物，发现罕见细胞类型、细胞亚型，揭示细胞之间的差异表达，成为研究复杂生物体系细胞异质性的有效方法，已广泛应用于干细胞、器官发育、神经系统、肿瘤、免疫、微生物等多个研究领域。近年来，该技术在眼科研究领域的应用也日渐增多。随着现代分子生物学与生物信息学的进展，高通量、低成本、高效的商业化测序平台不断涌现。本文主要阐述sc RNA-seq的几项重要技术应用的优缺点和该技术在眼科研究领域中的应用。在未来的研究中，该技术可能扩展到更多的眼部相关疾病的研究中，逐渐应用于临床，指导疾病的诊断及治疗。
Abstract:
Single-cell RNA-sequencing (sc RNA-seq) is a new technique allowing the analysis of transcriptomes in individual cell for discovering specific cell type markers，identifying rare cell types and cellular subsets and revealing the different expression among cells.It is becoming an effective method for researching cellular heterogeneity of complex biological system.In recent years，this approach has been widely used in diverse fields of biology，including stem cell，neurobiology，tumor，immunology，microbiology.In addition，it is also applied in ocular research area.As modern molecular biology and bioinformatics develops，there are some commercial platforms emerging gradually with characteristics of high-throughput，low cost and high efficiency.In this review，we will describe some of the current experimental methods used for sc RNA-seq and discuss the merits and drawbacks，as well as summarize the application of this technique in ophthalmology.In the future，this technique may expand to more studies of ocular-associated diseases for the guidance of clinical diagnosis and therapy.

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备注/Memo

备注/Memo:
国家重点基础研究发展计划资助项目（编号：2013CB967001）；国家自然科学基金项目（编号：31271400、31470065）
更新日期/Last Update: 2018-01-22