[1]易琼,邹文进.不同浓度多西环素对碱烧伤大鼠角膜中转化生长因子β1和基质金属蛋白酶9表达的影响[J].眼科新进展,2019,39(5):401-405.[doi:10.13389/j.cnki.rao.2019.0092]
　YI Qiong,ZOU Wen-Jin.Effects of different concentrations of doxycycline on the expression of transforming growth factor-β1 and matrix metalloproteinase-9 in alkali-burnt corneas of rats[J].Recent Advances in Ophthalmology,2019,39(5):401-405.[doi:10.13389/j.cnki.rao.2019.0092]
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不同浓度多西环素对碱烧伤大鼠角膜中转化生长因子β1和基质金属蛋白酶9表达的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
39卷
期数:
2019年5期
页码:
401-405
栏目:
实验研究
出版日期:
2019-05-05

文章信息/Info

Title:
Effects of different concentrations of doxycycline on the expression of transforming growth factor-β1 and matrix metalloproteinase-9 in alkali-burnt corneas of rats
作者:
易琼; 邹文进
530021　广西壮族自治区南宁市，广西医科大学第一附属医院眼科
Author(s):
YI Qiong; ZOU Wen-Jin
Department of Ophthalmology，the First Affiliated Hospital of Guangxi Medical University，Nanning 530021，Guangxi Zhuang Autonomous Region，China
关键词:
多西环素; 碱烧伤; 角膜; 转化生长因子-β1; 基质金属蛋白酶-9
Keywords:
doxycycline; alkali burn; cornea; transforming growth factor-β1; matrix metalloproteinase-9
分类号:
R772.21
DOI:
10.13389/j.cnki.rao.2019.0092
文献标志码:
A
摘要:
目的　探讨不同浓度多西环素对碱烧伤大鼠角膜中转化生长因子β1（transforming growth factor-β1，TGF-β1）和基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）表达的影响及其调节作用。方法　SD大鼠右眼制备成角膜碱烧伤模型，随机分为对照组、0.5 g·L-1多西环素组和1.0 g·L-1多西环素组，每组15只。3组分别于造模后每天给予20 μL眼液溶媒、0.5 g·L-1多西环素、1.0 g·L-1多西环素滴眼至观察期末。于碱烧伤后第3天、第7天、第14天对角膜混浊程度进行评分，并行RT-PCR和ELISA检测角膜组织中TGF-β1、MMP-9 mRNA和蛋白的表达情况。结果　与对照组相比，碱烧伤后第7天、第14天，0.5 g·L-1多西环素组［（2.80±0.45）分、（2.20±0.45）分］和1.0 g·L-1多西环素组［（2.00±0.00）分、（0.60±0.55）分］角膜混浊程度评分降低，且1.0 g·L-1多西环素组更低，差异均有统计学意义（均为P＜0.05）。碱烧伤后第3天、第7天、第14 天，0.5 g·L-1多西环素组、1.0 g·L-1多西环素组TGF-β1 mRNA及蛋白表达量均较对照组低，且1.0 g·L-1多西环素组表达更低，差异均有统计学意义（均为P＜0.05）。碱烧伤后第3天、第7天，0.5 g·L-1多西环素组、1.0 g·L-1多西环素组MMP-9 mRNA及蛋白表达均较对照组低，且1.0 g·L-1多西环素组表达更低，差异均有统计学意义（均为P＜0.05）。结论　多西环素可下调碱烧伤大鼠角膜中TGF-β1和MMP-9的表达，促进角膜愈合，并呈一定程度的剂量依赖性。
Abstract:
Objective　To investigate the effect of different concentrations of doxycycline on the expression of transforming growth factor-β1 （TGF-β1） and matrix metalloproteinase-9 （MMP-9） in rat alkali-burnt corneas and its regulation.Methods　The right eyes of SD rats were built for a corneal alkali burn model，then randomly divided into control group，0.5 g·L-1 doxycycline group and 1.0 g·L-1 doxycycline group，with 15 rats in each group.After the model was established，the three groups were respectively given 20 μL of menstruum eye drops，0.5 g·L-1 doxycycline and 1.0 g·L-1 doxycycline to the right eye every day until the end of the observation.At 3 days，7 days and 14 days after the model was established，the degree of corneal opacity was evaluated，and the expression of TGF-β1 and MMP-9 mRNA and protein in the corneal tissue was detected by RT-PCR and ELISA.Results　Compared with the control group，the score of corneal opacity was reduced in the 0.5 g·L-1 doxycycline group［（2.80±0.45）points，（2.20±0.45）points ］ and 1.0 g·L-1 doxycycline group ［（2.00±0.00）points，（0.60±0.55）points ］ at 7 days and 14 days after injury，and the 1.0 g·L-1 doxycycline group was instinctively lower，and the differences were statistically significant （all P<0.05）.The results of the RT-PCR and ELISA analyses demonstrated that the amounts of TGF-β1 mRNA and protein in the 0.5 g·L-1 doxycycline group and 1.0 g·L-1 doxycycline group were lower than those in the control group at 3 days，7 days and 14 days after the model was established，and the 1.0 g·L-1 doxycycline group was obviously lower，and the differences were statistically significant （all P<0.05）.The amounts of MMP-9 mRNA and protein in the 0.5 g·L-1 and 1.0 g·L-1 doxycycline group were lower than those in the control group at 3 days and 7 days after the model was established，and the 1.0 g·L-1 doxycycline group was significantly lower，and the differences were statistically significant （all P<0.05）.Conclusion　The effect of doxycycline on the alkali-burnt corneas of rats can downregulate the expression of TGF-β1 and MMP-9，and promote corneal healing in a dose-dependent manner.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：801160119）；广西自然科学基金资助（编号：2016GXNSF-AA380280、2017GXNSFAA198347）
更新日期/Last Update: 2019-05-14