[1]王军梅,孙艳,周明明,等.兔角膜碱烧伤后角膜基质注射脐带间充质干细胞的疗效[J].眼科新进展,2016,36(7):622-625.[doi:10.13389/j.cnki.rao.2016.0165]
　WANG Jun-Mei,SUN Yan,ZHOU Ming-Ming,et al.Effects of corneal stromal injection of umbilical cord mesenchymal stem cells after rabbit corneal alkali burn[J].Recent Advances in Ophthalmology,2016,36(7):622-625.[doi:10.13389/j.cnki.rao.2016.0165]
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兔角膜碱烧伤后角膜基质注射脐带间充质干细胞的疗效
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年7期
页码:
622-625
栏目:
实验研究
出版日期:
2016-07-05

文章信息/Info

Title:
Effects of corneal stromal injection of umbilical cord mesenchymal stem cells after rabbit corneal alkali burn
作者:
王军梅; 孙艳; 周明明; 丁敏; 邓爱军
261041　山东省潍坊市，潍坊医学院眼科教研室（王军梅，周明明）；261041　山东省潍坊市，潍坊医学院附属医院眼科中心（孙艳，丁敏，邓爱军）
Author(s):
WANG Jun-Mei ;  SUN Yan; ZHOU Ming-Ming ;  DING Min ;  DENG Ai-Jun
Department of Ophthalmology, Weifang Medical University ( WAWG Jun-Mei, ZHOU Ming-Ming ) , Weifang 261041 , Shandong Province. China, Department of Ophthalmology, the Affiliated Hospital of Weifang Medical University ( SUN Yan , DING Min , DENG Ai-Jun ) . Weifang 261041 , Shandong Province , China
关键词:
脐带间充质干细胞; 碱烧伤; 角膜新生血管; 角膜混浊; 角膜上皮缺损
Keywords:
umbilical cord mesenchymal stem cells ;  alkali burn ;  corneal neovascularization ;  corneal opacity ;  corneal epithelium defect
分类号:
Ｒ７７２
DOI:
10.13389/j.cnki.rao.2016.0165
文献标志码:
A
摘要:
目的　观察角膜基质注射脐带间充质干细胞（ｕｍｂｉｌｉｃａｌｃｏｒｄｍｅｓｅｎｃｈｙｍａｌｓｔｅｍｃｅｌｌｓ，ＵＭＳＣｓ）治疗兔角膜碱烧伤的效果。方法　健康新西兰大白兔随机分为３组，每组１２只，每只兔左眼为角膜碱烧伤模型眼。Ａ组兔左眼在碱烧伤２ｄ后角膜基质内注射含有２×１０６个ＵＭＳＣｓ的磷酸盐缓冲液２μＬ，Ｂ组注射相同剂量的磷酸盐缓冲液，Ｃ组为角膜碱烧伤后未进行处理组，在注射后不同时间对角膜混浊程度、新生血管生长情况、角膜上皮缺损情况等进行临床观察并评分，同时进行综合评分。结果　注射后１４ｄＡ组新生血管生长较Ｂ组明显缓慢，Ａ组、Ｂ组注射隧道周围角膜新生血管生长评分分别为０分、２分，差异有统计学意义（Ｐ＜０．０５）。注射后２ｄ和６ｄＡ组角膜混浊程度较Ｂ组明显轻，注射后２ｄＡ组、Ｂ组角膜混浊程度评分分别为２分、４分，注射后６ｄ评分与２ｄ相同，差异均具有统计学意义（均为Ｐ＜０．０５）。注射后２ｄ、７ｄ角膜上皮荧光素染色评分各组之间差异均无统学意义（均为Ｐ＞０．０５）。结论　碱烧伤后角膜基质注射ＵＭＳＣｓ可减少新生血管的形成，为抑制碱烧伤后角膜血管化提供参考，碱烧伤后角膜基质注射ＵＭＳＣｓ可在一定程度上恢复角膜透明度。
Abstract:
Objective To evaluate the efficacy of stromal injection of umbilical cord mesenchymal stem cells ( UMSCs) after rabbit corneal alkali burn. Methods Corneal alkali injury models were established in left eyes of healthy New Zealand rabbits .which were divided into three groups , 12 rats in each group. Group A was treated with intrastromal injections of phosphate-buffered saline ( PBS) 2 VL containing 2 x 106 UMSCs. Group B was treated with PBS by the same applications forms. Group C did not give any treatment after corneal alkali burn. Rabbits were evaluated for corneal neovascularization , opacification and epithelial defects at 2 days , 3 days ,6 days ,7 days , 14 days after injection. and scored on it according to the criteria, a comprehensive score was taken at the same time. Results At 14 days after injection , the growth of corneal neovascularization in group A was much slower than group B ,the mean corneal neovascularization area score in group A and group B was o points and 2 points ,respectively , the difference was statistically significant (P < 0. 05 ) . At 2 days and 6 days after injection, the corneal opacity of group A was less severe than group B , the corneal opacity score in group A and group B was all 2 points and 4 points,the differences were statistically significant ( all P < 0. 05 ) . Corneal fluorescence staining scores at 2 days ,7 days after injection between each group had no statistical significance ( P > 0. 05 ) . Conclusion Intrastromal injection of UMSCs after alkali burn cornea can reduce the formation of angiogenesis , which can provide a reference for the inhibition of corneal revascularization after alkali burn , and restore corneal transparency in a certain extent.

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更新日期/Last Update: 2016-07-12