[1]严京,严华,王谦,等.密蒙花方防护糖尿病大鼠早期视网膜神经细胞损伤机制探讨[J].眼科新进展,2018,38(12):1109-1113.[doi:10.13389/j.cnki.rao.2018.0262]
　YAN Jing,YAN Hua,WANG Qian,et al.The mechanism of the mimeng flower decoction to prevent early retinal nerve cell injury of diabetic rats[J].Recent Advances in Ophthalmology,2018,38(12):1109-1113.[doi:10.13389/j.cnki.rao.2018.0262]
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密蒙花方防护糖尿病大鼠早期视网膜神经细胞损伤机制探讨/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年12期
页码:
1109-1113
栏目:
实验研究
出版日期:
2018-12-05

文章信息/Info

Title:
The mechanism of the mimeng flower decoction to prevent early retinal nerve cell injury of diabetic rats
作者:
严京; 严华; 王谦; 葛东宇; 吴正正; 接传红; 宫喜梅; 宋小花
100040　北京市，中国中医科学院眼科医院（严京，吴正正，接传红，宫喜梅，宋小花）；100050　北京市，中国食品药品检定研究院（严华）；100029　北京市，北京中医药大学（王谦，葛东宇）
Author(s):
YAN Jing; YAN Hua; WANG Qian; GE Dong-Yu; WU Zheng-Zheng; JIE Chuan-Hong; GONG Xi-Mei; SONG Xiao-Hua
Eye Hospital of China Academy of Traditional Chinese Medical Science（YAN Jing，WU Zheng-Zheng，JIE Chuan-Hong，GONG Xi-Mei，SONG Xiao-Hua），Beijing 100040，China；National Institutes for Food and Drug Control （YAN Hua），Beijing 100050，China；Beijing University of Chinese Medicine（WANG Qian，GE Dong-Yu），Beijing 100029，China
关键词:
密蒙花方; 糖尿病视网膜病变; 视网膜神经细胞; 谷氨酸; Sigma-1R
Keywords:
Mimeng flower decoction; diabetic retinopathy; retinal ganglion cell; glutamate; Sigma-1R
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2018.0262
文献标志码:
A
摘要:
目的　探讨密蒙花方防治糖尿病视网膜病变大鼠早期视网膜神经细胞损伤的作用机制，为临床实践提供实验依据。方法　采用清洁级雄性Sprague-Dawley（SD） 大鼠120只，其中正常组30只，其余大鼠采用链脲佐菌素诱导糖尿病大鼠动物模型，造模成功后随机分为模型组及密蒙花方组，密蒙花方组采用密蒙花方灌胃3个月，模型组用与密蒙花方组等容的蒸馏水灌胃。给药1个月后，采用TUNEL法检测大鼠视网膜神经节细胞（retinal ganglion cells，RGCs）凋亡情况；分别在给药1个月、2个月、3个月后采用高效液相色谱法检测各组大鼠视网膜中谷氨酸含量；PCR以及Western blot法检测视网膜中Sigma-1R mRNA和蛋白表达情况。结果　给药1个月后，TUNEL法检测结果示，密蒙花方组RGCs凋亡数少于模型组。高效液相色谱法检测结果示，给药1个月、2个月、3个月后，模型组较正常组谷氨酸含量均增加（均为P＜0.01），密蒙花方组谷氨酸含量较模型组均降低（均为P＜0.01）。给药3个月后，正常组、模型组和密蒙花方组视网膜Sigma-1R mRNA相对表达量分别为1.71±0.11、0.67±0.09和1.50±0.14，Sigma-1R蛋白相对表达量分别为0.39±0.02、0.16±0.02和0.30±0.01；与正常组相比，模型组Sigma-1R mRNA及蛋白水平均降低（均为P＜0.01）；与模型组相比，密蒙花方组Sigma-1R mRNA及蛋白水平均升高（均为P＜0.01）；密蒙花方组与正常组之间差异均无统计学意义（均为P>0.05）。结论　糖尿病视网膜病变早期即出现视网膜神经细胞的凋亡及损伤，密蒙花方可通过谷氨酸和Sigma-1R介导机制干预糖尿病大鼠早期视网膜神经细胞损伤，从而起到阻止糖尿病视网膜病变进一步发展的作用。
Abstract:
Objective　To study the mechanism of the mimeng flower decoction to prevent and treat early diabetic retinopathy rats retinal nerve cell injury，to provide experimental basis for clinical practice.Methods　Totally 120 clean level male Sprague-Dawley（SD） rats，in which 30 rats were normal group，and others were used streptozototin（STZ），After successful modeling，randomly divided into model group and mimeng flower group.In mimeng flower group，mimeng flower decoction were administered for 3 months.In model group，distilled water with the same capacity was administered.After one month，apoptosis of retinal ganglion cells in rats were detected by TUNEL.After one month，two months，three months，the content of glutamate in retinal tissue was determined by high performance liquid chromatography.The expression of Sigma-1R mRNA and protein in retina were detected by PCR and Western blot.Results　STZ-induced diabetic rats with a stable and persistent hyperglycemic state showed typical symptoms of a little more than three.After one month，apoptosis of retinal ganglion cells in diabetic rats were detected by TUNEL method，and the numbers of apoptosis cells in miming flower group were less than that in model group.The results of glutamate contents in retinal tissue detected by high-performance liquid chromatography showed that the contents of glutamate increased in the model group compared with that in the normal group（P＜0.01）.After the drugs were used，the contents of glutamate in the mimeng flower group were reduced compared with the model group（P＜0.01）.The contents of glutamate in normal group reached the highest after two months，then decreased，while the contents of glutamate in model group and mimeng flower group increased gradually time dependent.The relative expressions of Sigma-1R mRNA in the normal group，the model group and the mimeng flower group respectively were 1.71±0.11，0.67±0.09 and 1.50±0.14.About the ratio of the retinal Sigma-1R protein expressed to the Sigma-1R/β-actin，the normal group，the model group and the mimeng flower group respectively were 0.39±0.02，0.16±0.02 and 0.30±0.01.Compared with the normal group，the level of Sigma-1R mRNA and protein in the model group decreased（P＜0.01），and the level of Sigma-1R mRNA and protein in the mimeng flower group increased compared with the model group（P＜0.01）.There were no significant difference between mimeng flower group and normal group（P＞0.05）.Conclusion　The damage to retinal nerve cells has been happened in the early stage of diabetic retinopathy.mimeng flower decoction could intervene in the early retinal nerve cell injury in diabetic rats through glutamate mechanism and Sigma-1 mediated mechanism，thus playing a role in preventing the further development of the disease.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81001540）
更新日期/Last Update: 2018-12-04