[1]郑浩,李根林.碱性成纤维细胞生长因子（bFGF）在遗传性视网膜变性小鼠视网膜内的表达[J].眼科新进展,2017,37(5):411-414.[doi:10.13389/j.cnki.rao.2017.0104]
　ZHENG Hao,LI Gen-Lin.A contrastive research on bFGF expression in retinas of rd mouse and C57BL mouse[J].Recent Advances in Ophthalmology,2017,37(5):411-414.[doi:10.13389/j.cnki.rao.2017.0104]
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碱性成纤维细胞生长因子（bFGF）在遗传性视网膜变性小鼠视网膜内的表达/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年5期
页码:
411-414
栏目:
实验研究
出版日期:
2017-05-05

文章信息/Info

Title:
A contrastive research on bFGF expression in retinas of rd mouse and C57BL mouse
作者:
郑浩; 李根林
030002　山西省太原市，山西省眼科医院眼底病科（郑浩）；100730　北京市，首都医科大学附属北京同仁医院眼科中心，北京市眼科学与视觉科学重点实验室（李根林）
Author(s):
ZHENG Hao; LI Gen-Lin
Department of Ocular Fundus Diseases，the Shanxi Eye Hospital（ZHENG Hao），Taiyuan 030002，Shanxi Province，China；Beijing Tongren Eye Center，Beijing Tongren Hospital，Capital Medical University，Beijing Ophthalmology & Visual Sciences Key Lab（LI Gen-Lin），Beijing 100730，China
关键词:
碱性成纤维细胞生长因子; 视网膜变性; 神经节细胞
Keywords:
basic fibroblast growth factor; retinal degeneration; ganglion cell
分类号:
R774.1
DOI:
10.13389/j.cnki.rao.2017.0104
文献标志码:
A
摘要:
目的　比较碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）在出生后五个不同周龄的rd小鼠（病变组，遗传性视网膜变性小鼠）和C57BL小鼠（正常对照组）视网膜内的表达，分析小鼠视网膜发育成熟或变性的过程中bFGF表达的异同及变化特点，进而推测bFGF在视网膜神经细胞发育分化或退变中可能的作用。方法　随机选取出生后0、1、2、3、4周龄的病变组小鼠和正常对照组小鼠各8只即刻处死，摘除眼球制作视网膜切片，应用免疫组织化学法和光学显微镜法及半定量分析法对视网膜内bFGF的表达量进行测定。结果　两组小鼠出生后4周内的bFGF含量曲线特点基本相同：均在出生后开始下降，至出生后1周龄时达到低谷；随后开始上升，至出生后2周龄时达到高峰，此时两组小鼠视网膜的十层结构均基本形成；2周龄后开始二次下降，直至出生后4周龄，在此期间，病变组小鼠的视网膜迅速退变，而正常对照组小鼠的视网膜继续发育成熟。在出生后 0～4周各周龄，bFGF在病变组小鼠视网膜内的表达量均明显低于同龄正常对照组小鼠视网膜内的表达量，差异均有统计学意义（P＜0.01）。结论　bFGF可能在视网膜神经细胞的分化成熟和形态维持中起一定的保护作用，而视网膜内bFGF处于较高表达水平可能在一定程度上能防止其退变凋亡。
Abstract:
Objective　To compare the expressions of basic fibroblast growth factor （bFGF） in retinas of rd mouse （the experiments） and C57BL mouse （the controls） of five different postnatal ages，analyze the similarities and differences of bFGF expressions，and conclude the role of bFGF during the key period that retinas developed or degenerated.Methods　Eight rd mice and eight C57BL mice of postnatal 0 week，1 week，2 weeks，3 weeks，4 weeks （PN 0，1，2，3，4 W） were chosen stochastically and executed immediately，their eyeballs were excavated and made into the retinal slices，then the bFGF contents in the retinas were analyzed by immunohistochemical method，photomicroscope and semi-quantitative analysis.Results　Change characteristic of the curve of bFGF contents in retina of rd mice was similar to that of C57BL mice.Both curves began to fall after born，which reached each low level at PN-1W and then rose until reaching each peak at PN-2W，at which ten layer structures of retinas in both groups were formed.Then both curves began the second fall till PN-4W.During the last two weeks，retinas of C57BL mice degenerated rapidly while retinas of rd mice continued to mature.For all the five postnatal weeks，the bFGF contents in retinas of rd mice were lower than those of C57BL mice evidently.All statistical analysis of the difference have made sense distinctly （P<0.01）.Conclusion　bFGF may play a protective role in the developing to maturation and sustaining of retinal structure，and the higher expression of bFGF content may prevent retinal degeneration in some extent.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81271046）；北京市自然科学基金项目（编号：KZ201510025025）
更新日期/Last Update: 2017-06-07