[1]高亚莉,陆晓和.lncRNA-MEG3通过P53途径介导视网膜母细胞瘤凋亡[J].眼科新进展,2017,37(4):301-304.[doi:10.13389/j.cnki.rao.2017.0076]
　GAO Ya-Li,LU Xiao-He.LncRNA-MEG3 mediated apoptosis of retinoblastoma by regulating P53 pathway[J].Recent Advances in Ophthalmology,2017,37(4):301-304.[doi:10.13389/j.cnki.rao.2017.0076]
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年4期
页码:
301-304
栏目:
实验研究
出版日期:
2017-04-05

文章信息/Info

Title:
LncRNA-MEG3 mediated apoptosis of retinoblastoma by regulating P53 pathway
作者:
高亚莉; 陆晓和
518020　广东省深圳市，深圳市人民医院(暨南大学第二临床医学院)眼科（高亚莉）；510280广东省广州市，南方医科大学珠江医院眼科(陆晓和)
Author(s):
GAO Ya-Li; LU Xiao-He
Department of Ophthalmology，Shenzhen People’s Hospital(The Second Clinical Medical College)，Jinan University（GAO Ya-Li），Shen-zhen 518020，Guangdong Province，China；the Department of Ophthalmology，Zhujiang Hospital of Southern Medical University（LU Xiao-He），Guangzhou 510280，Guangdong Province，China
关键词:
视网膜母细胞瘤; 长链非编码RNA; MEG3; 细胞凋亡; P53蛋白
Keywords:
retinoblastoma; long non-coding RNA; MEG3; cell apoptosis; P53 protein
分类号:
R774
DOI:
10.13389/j.cnki.rao.2017.0076
文献标志码:
A
摘要:
目的　探讨MEG3是否参与视网膜母细胞瘤的形成及其分子机制。方法　应用实时荧光定量PCR技术检测视网膜母细胞瘤组织及瘤旁正常视网膜组织标本中MEG3的表达水平；通过转染pcDNA-MEG3或siRNA-MEG3上调或干扰视网膜母细胞瘤细胞SO-RB50及HXO-RB44中MEG3的表达水平，随后用流式细胞仪检测转染后的细胞凋亡率变化，用Western blot检测转染前后P53蛋白表达水平的变化。结果　视网膜母细胞瘤组织中MEG3的表达较瘤旁正常视网膜组织出现显著下降（P=0.014)。转染了pcDNA-MEG3的SO-RB50细胞MEG3表达显著增加（P=0.002)，转染了siRNA-MEG3的HXO-RB44细胞MEG3表达显著减少（P=0.004)。流式细胞仪检测结果显示，MEG3表达上调后的SO-RB50细胞凋亡率显著增加（P＜0.05)；干扰MEG3表达后的HXO-RB44细胞凋亡率显著减少（P＜0.05)。Western blot检测结果显示，转染了pcDNA-MEG3的SO-RB50细胞中P53蛋白的表达较阴性对照组显著增加（P＜0.05)，转染了siRNA-MEG3的HXO-RB44细胞中P53蛋白的表达较阴性对照组显著减少（P＜0.05)。结论　视网膜母细胞瘤组织中MEG3的表达下调，且MEG3可能通过促进P53蛋白的表达诱导视网膜母细胞瘤细胞的凋亡，从而影响视网膜母细胞瘤的发生和发展。
Abstract:
Objective　To investigate whether MEG3 involved in the development of retinoblastoma by down-regulating the expression of P53 protein.Methods　The MEG3 expression of retinoblastoma tissues and corresponding non-tumor tissues were detected by quantitative real-time PCR (qRT-PCR).Retinoblastoma cell lines SO-RB50 or HXO-RB44 were transfected with pcDNA-MEG3 or siRNA-MEG3,after which cell apoptosis was tested by flow cytometry and P53 protein expression was tested by Western blot.Results　MEG3 expression of retinoblastoma tissues was significantly reduced compared with corresponding non-tumor tissues（P=0.014).MEG3 level was significantly increased in pcDNA-MEG3 transfected SO-RB50 cells (P=0.002) and significantly decreased in siRNA-MEG3 transfected HXO-RB44 cells (P=0.004).Flow cytometry showed that the SO-RB50 cells apoptosis was significantly increased with the MEG3 over-expression（P＜0.05),as well as the HXO-RB44 cells apoptosis was significantly decreased with the MEG3 knockdown（P＜0.05),compared with the control group,respectively.Furthermore,Western blot showed that P53 protein level was significantly increased after SO-RB50 transfected with pcDNA-MEG3（P＜0.05),while significantly decreased after HXO-RB44 transfected with siRNA-MEG3（P＜0.05),compared with the control group,respectively.Conclusion　MEG3 is down-regulated in retinoblastoma,affect the development of retinoblastoma,and may induce the retinoblastoma cell apoptosis by promoting the expression of P53 protein.

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备注/Memo

备注/Memo:
国家自然科学基金面上项目（编号：81371067）；深圳市卫生计生系统科研项目（编号：201507010）
更新日期/Last Update: 2017-04-26