[1]李娟,丁小艳,王亚虹,等.PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响[J].眼科新进展,2017,37(3):201-204.[doi:10.13389/j.cnki.rao.2017.0052]
　LI Juan,DING Xiao-Yan,WANG Ya-Hong,et al.Effects of PM2.5 on tear film function and corneal epithelial structure in mice[J].Recent Advances in Ophthalmology,2017,37(3):201-204.[doi:10.13389/j.cnki.rao.2017.0052]
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PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年3期
页码:
201-204
栏目:
实验研究
出版日期:
2017-03-05

文章信息/Info

Title:
Effects of PM2.5 on tear film function and corneal epithelial structure in mice
作者:
李娟; 丁小艳; 王亚虹; 叶鳞泓; 叶蕾; 姜楠; 邵毅
710004　陕西省西安市，西安市第四医院眼科（李娟）;710003　陕西省西安市，西安市第二医院眼科（丁小艳）；710054　陕西省西安市，西安市环境监测站（王亚虹）；330006　江西省南昌市，南昌大学第一附属医院眼科（叶鳞泓，叶蕾，姜楠，邵毅）
Author(s):
LI Juan; DING Xiao-Yan; WANG Ya-Hong; YE Lin-Hong; YE Lei; JIANG Nan; SHAO Yi
Department of Ophthalmology,the Fourth Hospital of Xi’an（LI Juan）,Xi’an 710004,Shaanxi Province,China；Department of Ophthalmology,the Second Hospital of Xi’an（DING Xiao-Yan）,Xi’an 710003,Shaanxi Province,China；Environmental Monitoring Station of Xi’an City（WANG Ya-Hong），Xi’an 710054,Shaanxi Province,China；Department of Ophthalmology,the First Affiliated Hospital of Nanchang University(YE Lin-Hong,YE Lei,JIANG Nan,SHAO Yi),Nanchang 330006,Jiangxi Province,China
关键词:
PM2.5; 泪膜功能; 角膜上皮; 小鼠
Keywords:
PM2.5; tear film function; corneal epithelium; mouse
分类号:
R772;R777
DOI:
10.13389/j.cnki.rao.2017.0052
文献标志码:
A
摘要:
目的　观察PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响。方法　24只雄性6~8周龄BALB/c小鼠，随机分为A、B两组，每组12只。B组采用5 mg·mL-1 PM2.5混悬液滴眼，A组采用PBS滴眼，每天4次。分别在干预后1 d、4 d、7 d对各组小鼠进行泪膜功能检测，包括泪液分泌功能、泪膜破裂时间(break-up time,BUT)、荧光素染色(fluorescein staining,FL)，并于干预后7 d行苏木精-伊红染色观察角膜上皮情况。结果　干预后4 d、7 d，A组泪液分泌量、BUT较干预前无明显变化，差异均无统计学意义（均为P＞0.05)，而B组泪液分泌量、BUT较干预前明显分别减少和恶化，差异均有统计学意义（均为P＜0.05)。干预后7 d，A组FL评分较干预前无明显变化，差异无统计学意义（P>0.05），而B组FL评分较干预前明显增加，差异有统计学意义（P=0.003）。干预后7 d，A 组上皮细胞层数为（4±1）层，而B 组上皮细胞层数为（7±1）层，差异有统计学意义（P＜0.05）。与A组比较，B组整个角膜FL着染明显增加，角膜表面上皮细胞层损伤，层数增厚。结论　PM2.5会影响小鼠泪膜功能，损伤小鼠角膜上皮的组织结构。
Abstract:
Objective　To investigate the effects of PM2.5 on tear film function and corneal epithelial structure in mice.Methods　Totally 24 male BALB/c mice (24 eyes) were divided into two groups:group A (with PBS eye drops,n=12),group B (5 mg·mL-1 PM2.5 eye drop group,n=12).PBS and PM2.5 eye drop were given with four times per day for 7 consecutive days in right eye.Tear secretion level was measured with phenol red thread.Break-up time (BUT) of tear film was tested,and corneal fluorescein staining (FL) was scored before therapy and 1 day,4 days and 7 days after droppings and HE staining was performed 7 days after droppings,respectively.Results　There was no significant difference in the tear secretion levels,BUT,FL between the groups A and B before treatment (all P>0.05).At 4 days,7 days after treatment with PM2.5,the mean differences of the group B showed all items significantly changed compared with those before treatment (all P<0.05).For the group A,there was no statistical change in tear secretion levels,BUT,FL at 7 days after treatment (all P>0.05).There were statistical differences in all items between group A and B at each time point (all P<0.05).At 7 days after therapy,the mean layers of corneal epithelial cells in the group A (4±1) was significantly lower than that in the group B (7±1)（P<0.05）.The group B showed that the whole corneal fluorescein staining obviously increased,and corneal epithelial cell layer was thickened.Conclusion　PM2.5 can influence tears film function and damage the corneal epithelial structure in mice.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编 号：81160118、81400372、81400424、81660158）；陕西省科学技术研究发展计划项目(编号：2014K11-03-07-04)；2017年陕西省创新人才推进计划项目；江西省远航工程（编号：2014022）；江西省自然科学基金重大项目（编号：2016ACB21017）；江西省青年科学基金（编号：20151BAB215016）；江西省科技支撑计划项目（编号：20151BBG70223）
更新日期/Last Update: 2017-03-30