[1]李娟,郝江东,乔妮妮,等.环境烟草烟雾对小鼠泪膜功能和角膜上皮组织结构的影响[J].眼科新进展,2020,40(6):516-519.[doi:10.13389/j.cnki.rao.2020.0118]
　LI Juan,HAO Jiangdong,QIAO Nini,et al.Influence of environmental tobacco smoke on tear film function and structure of corneal epithelial tissues in mice[J].Recent Advances in Ophthalmology,2020,40(6):516-519.[doi:10.13389/j.cnki.rao.2020.0118]
点击复制

环境烟草烟雾对小鼠泪膜功能和角膜上皮组织结构的影响/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
40卷
期数:
2020年6期
页码:
516-519
栏目:
实验研究
出版日期:
2020-06-05

文章信息/Info

Title:
Influence of environmental tobacco smoke on tear film function and structure of corneal epithelial tissues in mice
作者:
李娟; 郝江东; 乔妮妮; 罗阿丽; 张光伟; 李勇; 周颖; 陈旭洋; 年申
710004　陕西省西安市，西安市第四医院眼科（李娟，李勇）；250021　山东省济南市，山东大学附属省立医院眼科（郝江东）；710021　陕西省西安市，西安医学院公共卫生学院（乔妮妮，张光伟）；710100　陕西省西安市，西安市胸科医院病理科（罗阿丽）；415003　湖南省常德市，常德市第一人民医院眼科(周颖)；325035　浙江省温州市，温州医科大学眼视光医学院（陈旭洋）；710021　陕西省西安市，西安医学院基础医学部病理教研室（年申）
Author(s):
LI Juan1; HAO Jiangdong2; QIAO Nini3; LUO Ali4; ZHANG Guangwei3; LI Yong1; ZHOU Ying5; CHEN Xuyang6; NIAN Shen7
1.Department of Ophthalmology,Xi’an Fourth Hospital
2.Department of Ophthalmology,Shandong Provincial Hospital,Cheelo College of Medicine,Shandong University
3.School of Public Health,Xi’an Medical University
4.Department of Pathology,the Chest Hospital of Xi’an
5.Department of Ophthalmology,the First People’s Hospital of Changde
6.School of Ophthalmology,Wenzhou Medical University
7.Pathological Department,Faculty of Basic Medicine,Xi’an Medical University
关键词:
环境烟草烟雾; 泪膜功能; 角膜上皮
Keywords:
environmental tobacco smoke;  tear film function;  corneal epithelial tissues
分类号:
R772.2
DOI:
10.13389/j.cnki.rao.2020.0118
文献标志码:
A
摘要:
目的　探讨环境烟草烟雾（enviromental tobacco smoke，ETS）对小鼠泪膜功能和角膜上皮组织结构的影响。方法　选取SPF级c57BL雄性小鼠12只，随机分为对照组和ETS干预组，每组6只。对照组不进行ETS干预，ETS干预组进行ETS干预，每天2次，一次30 min，干预12周。在干预前及干预后对各组小鼠进行泪膜功能检测，包括泪膜破裂时间、荧光素染色(FL)评分；干预后摘取小鼠角膜组织，行HE染色，在光学显微镜下观察小鼠角膜上皮结构变化情况。结果　干预12周后，对照组泪膜破裂时间和FL评分与干预前相比，差异均无统计学意义(均为P>0.05)；而ETS干预组泪膜破裂时间较干预前明显缩短，FL评分较干预前明显增加，差异均有统计学意义（均为P<0.05)。ETS干预组干预12周后泪膜破裂时间较对照组明显缩短，FL评分较对照组明显增加，差异均有统计学意义(均为P<0.05)；两组干预前泪膜破裂时间和FL评分差异均不明显（均为P>0.05）。FL染色结果显示：干预12周后，对照组小鼠角膜上皮完整，角膜染色呈阴性；ETS干预组整个角膜上皮荧光素着染区域明显增加，呈点片状。HE染色结果显示：干预12周后，对照组未见角膜上皮层数的改变，厚度也基本未变，基底细胞仍为单层柱状上皮细胞，表层上皮较完整；ETS干预组可见角膜上皮细胞层数增多，厚度增加，细胞排列紊乱，表层上皮细胞有损失及脱落，角膜表面不光滑。干预前对照组小鼠上皮细胞为（5±1）层，干预后基本不变；ETS干预组干预后为（7±1）层；干预后两组上皮细胞层数比较差异有统计学意义（P＜0.05）。结论　ETS会影响小鼠泪膜功能，损伤小鼠角膜上皮的组织结构。
Abstract:
Objective　To investigate the influence of environmental tobacco smoke (ETS) on tear film function and structure of corneal epithelial tissues in mice. Methods　Totally 12 male c57BL mice were randomly divided into control group and ETS group, 6 mice in each group. Mice in control group did not receive ETS intervention, and those in ETS group were intervened by ETS two times a day for 12 weeks, 30 minutes each. Tear film function was detected before and after ETS intervention, including break-up time of tear film (BUT), corneal florescence staining (FL) score, etc. Corneal tissues were removed after intervention, and received HE staining. Changes of corneal epithelial tissues were observed under light microscope. Results　Compared with that before intervention, there was no significant difference in BUT or FL score for control group at 12-week intervention (both P>0.05); while BUT obviously reduced and FL score obviously increased in ETS group at 12-week intervention (both P<0.05). Compared with control group, BUT obviously reduced and FL score obviously increased in ETS group after 12-week intervention, but the differences were statistically significant (all P<0.05). There was no statistical difference in BUT or FL score between the two groups before intervention (all P>0.05). FL staining results showed: corneal epithelium was intact and corneal staining was negative in control group after 12-week intervention; fluorescein staining area in the whole corneal epithelium was obviously increased in dot-flake shape in ETS group at 12-week intervention. HE staining results showed: there was no change in number or thickness of corneal epithelial layers, basal cells were single-layer columnar epithelial cells and surface epithelium was intact in control group after 12-week intervention. In ETS group, number and thickness of corneal epithelial cell layers increased, there was disordered cell arrangement, surface epithelial cell loss or falling off, and unsmooth corneal surface. The number of epithelial cell layers was 5±1 before intervention and basically unchanged after intervention in control group; the number of epithelial cell layers was 7±1 after intervention in ETS group; statistical difference was found in number of epithelial cell layers after intervention between the two groups (P<0.05). Conclusion　 ETS can influence tears film function in mice and damage the structure of corneal epithelial tissues.

参考文献/References:

［1］　胡祥炬，林曙光，钟文玲，陈铁晖.烟草流行现状及应对策略［J］.海峡预防医学杂志，2013，19(1)：70-72.
HU X J,LIN S G,ZHONG W L,CHEN T H.Tobacco epidemic situation and countermeasures［J］.J Chann Prevent Med，2013，19(1)：70-72.
［2］　世界卫生组织.防止二手烟暴露:政策建议［R］.日内瓦:世界卫生组织，2007.
World Health Organization.Prevention of secondhand smoke exposure:policy recommendations［R］.Geneva:WHO，2007.
［3］　中国疾病预防控制中心.中国成人烟草调查报告［R］.北京:中国疾病预防控制中心,2015.
China Centers for Disease Control and Prevention.China adult tobacco survey［R］.Beijing:China Centers for Disease Control and Prevention,2015.
［4］　YU Y,ZOU J,HAN Y,HE H,HU P,SHAO Y,et al.Effects of intravitreal injection of netrin-1 in retinal neovasculariza-tion of streptozotocin-induced diabetic rats［J］．Drug Des Devel Ther,2015,9:6363-6377．
［5］　LIN Z,LIU X,ZHOU T,WANG Y,BAI L,HE H,et al.A mouse dry eye model induced by topical administration of benzalko-nium chloride［J］.Mol Vis,2011,17:257-264．
［6］　LI J,TAN G,DING X Y,WANG Y H,WU A H,YANG Q C,et al.A mouse dry eye model induced by topical administration of the air pollutant particulate matter 10［J］.Biomed Pharmacother,2017,96:524-534.
［7］　SHAH A S,LANGRISH J P,NAIR H,MCALLISTER D A,HUNTER A L,DONALDSON K,et al.Global association of air pollution and heart failure:a systematic review and meta-analysis［J］.Lancet,2013,382(9897):1039-1048.
［8］　Department of Health and Human Services.The health consequences of involuntary exposure to tobacco smoke.a report of the surgeon general:2006［R］.Washington DC:US Government Printing Office,2006.
［9］　中华全科医学编辑部.无烟生活，健康中国［J］.中华全科医学，2016，14(12):2010.
Editorial department of Chinese General Medicine.Smokeless life healthy China［J］.Chin Gener Med，2016,14(12):2010.
［10］　KIM S,KANG S H,HAN D,KIM S H,KIM H J,PARK J J,et al.Relation between secondhand smoke exposure and cardiovascular risk factors in never smokers［J］.J Hypertens,2017,35(10):1976-1982.
［11］　SANDLER D P,COMSTOCK G W,HELSING K J,SHORE D L.Deaths from all causes in non-smokers who lived with smokers［J］.Am J Public Health,1989,79(2):163-167.
［12］　ROBBINS A S,ABBEY D E,LEBOWITZ M D.Passive smoking and chronic respiratory disease,symptoms in non-smoking adults［J］.Int J Epidemiol,1993,22(5):809-817.
［13］　DAVIES D P,ABERNETHY M.Cigarette smoking in pregnancy:associations with maternal weight gain and fetal growth［J］.Lancet,1976,1(7956):385-387.
［14］　张建辰，谢振斌，宰守峰，刘家霖,李家夫,刘吉宝.河南新乡地区女性乳腺癌危险因素的病例对照研究［J］.河南肿瘤学杂志，2003，16(3):201-203.
ZHANG J C,XIE Z B,ZAI S F,LIU J S,LI J F,LIU J B.Case-control study on female breast cancer risk factors in xinxiang,henan［J］.Henan J Oncol，2003，16(3):201-203.
［15］　马玉山，续喜涛.被动吸烟对人群健康的影响［J］.职业与健康,2004,20(3):13.
MA Y S,XU X T.Effects of passive smoking on population health［J］.Occupat Health，2004,20(3):13.
［16］　LOIS N,ABDELKADER E,REGLITZ K,GARDEN C,AYRES J G .Environmental tobacco smoke exposure and eye disease［J］.Br J Ophthalmol,2008,92(10):1304-1310.
［17］　CWIKLIK L.Tear film lipid layer:a molecular level view［J］.Biochim Biophys Acta,2016,1858(10):2421-2430.
［18］　RUMMENIE V T,MATSUMOTO Y,DOGRU M,WANG Y,HU Y,WARD S K,et al.Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure［J］.Cytokine,2008,43(2):200-208.
［19］　LI L,BRAUN R J,MAKI K L,HENSHAW W D,KING-SMITH P E .Tear film dynamics with evaporation,wetting,and time-dependent flux boundary condition on an eye-shaped domain［J］.Phys Fluids,2014,26 (5):052101．
［20］　邵毅，余瑶，裴重刚，童桂芳，周琼，贺鹏，等． Pranoprofen 胶囊治疗造血干细胞移植术后慢性移植物抗宿主病引起的中重度干眼症临床研究［J］.中国实用眼科杂志，2013，31(5):536-541．
SHAO Y，YU Y，PEI C G，TONG G F，ZHOU Q ，HE P，et al．Clinical study on pranoprofen drops of dry eye syndrome caused by chronic graft versus host disease after hematopoietic stem cell transplantation［J］.Chin J Pract Ophthalmol,2013,31(5):536-541．
［21］　邵毅，余瑶，黄国栋，谭钢，裴重刚，刘新华． 鬼针草叶治疗更年期女性中重度干眼症临床研究［J］.中国中药杂志,2012,37(19):2985-2989．
SHAO Y，YU Y，HUANG G D，TAN G，PEI C G，LIU X H．Clinical study on spanishneedles leaves in treatment of middle and severe xerophthalmia of menopausal females［J］.Chin J Tradit Chin Med，2012,37(19):2985-2989．
［22］　LU H,WANG M R,WANG J,SHEN M.Tear film measurement by optical reflectometry technique［J］.J Biomed Opt,2014,19(2):027001．
［23］　MATSUMOTO Y,DOGRU M,GOTO E,SASAKI Y,INOUE H,SAITO I,et al.Alterations of the tear film and ocular surface health in chronic smokers［J］.Eye (Lond),2008,22(7):961-968.

相似文献/References:

[1]李娟,丁小艳,王亚虹,等.PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响[J].眼科新进展,2017,37(3):201.[doi:10.13389/j.cnki.rao.2017.0052]
　LI Juan,DING Xiao-Yan,WANG Ya-Hong,et al.Effects of PM2.5 on tear film function and corneal epithelial structure in mice[J].Recent Advances in Ophthalmology,2017,37(6):201.[doi:10.13389/j.cnki.rao.2017.0052]
[2]张厚检,叶蕾,朱佩文,等.蒙脱石滴眼液对小鼠泪膜功能和角膜上皮厚度的影响[J].眼科新进展,2018,38(9):810.[doi:10.13389/j.cnki.rao.2018.0192]
　ZHANG Hou-Jian,YE Lei,ZHU Pei-Wen,et al.Effects of montmorillonite eye drops on tear film function and corneal epithelial thickness[J].Recent Advances in Ophthalmology,2018,38(6):810.[doi:10.13389/j.cnki.rao.2018.0192]
[3]李娟,周颖,谭钢,等.间充质干细胞外泌体治疗小鼠干眼的疗效评价[J].眼科新进展,2019,39(10):901.[doi:10.13389/j.cnki.rao.2019.0205]
　LI Juan,ZHOU Ying,TAN Gang,et al.Therapeutic effects of mesenchymal stem cells derived exosomes on dry eye mice[J].Recent Advances in Ophthalmology,2019,39(6):901.[doi:10.13389/j.cnki.rao.2019.0205]
[4]李娟,周颖,李康寯,等.角膜基质透镜泪道栓治疗兔干眼的实验研究[J].眼科新进展,2019,39(11):1014.[doi:R777.3]
　LI Juan,ZHOU Ying,LI Kang-Jun,et al.Corneal stromal lenticule punctal plug for the treatment of dry eye in rabbits[J].Recent Advances in Ophthalmology,2019,39(6):1014.[doi:R777.3]
[5]郭宣妮,姜春辉,朱安泰,等.青光眼术后干眼患者泪液中MAPK、Sirt1水平与泪膜功能的相关性分析[J].眼科新进展,2020,40(7):663.[doi:10.13389/j.cnki.rao.2020.0152]
　GUO Xuanni,JIANG Chunhui,ZHU Antai,et al.Correlations between MAPK, Sirt1 levels in lacrimal gland and tear film function in dry eye patients after glaucoma surgery[J].Recent Advances in Ophthalmology,2020,40(6):663.[doi:10.13389/j.cnki.rao.2020.0152]
[6]翁铭,张云,杨卫华.Oculus眼表综合分析仪在复发性翼状胬肉切除联合自体角膜缘干细胞移植术后眼表功能评价中的应用[J].眼科新进展,2020,40(8):765.[doi:10.13389/j.cnki.rao.2020.0174]
　WENG Ming,ZHANG Yun,YANG Weihua.Evaluation of tear film changes after recurrent pterygium surgery with limbal stem cell autografts transplantation using oculus keratograph[J].Recent Advances in Ophthalmology,2020,40(6):765.[doi:10.13389/j.cnki.rao.2020.0174]

备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81400424）；陕西省科学技术研究发展计划项目(编号：2014K11-03-07-04)；2017年陕西省创新人才推进计划项目-青年科技新星项目(编号：2017KJXX-87)；西安医学院博士科研启动基金（编号：2016DOC24）
更新日期/Last Update: 2020-06-05