[1]郭切,李志远,荆凡波.Nod样受体蛋白-3（NLRP3）炎症小体在翼状胬肉中的表达[J].眼科新进展,2017,37(2):125-128.[doi:10.13389/j.cnki.rao.2017.0033]
　GUO Qie,LI Zhi-Yuan,JING Fan-Bo.Expression of NOD-like receptor pyrin domain-3（NLRP3）inflammasome in pterygium[J].Recent Advances in Ophthalmology,2017,37(2):125-128.[doi:10.13389/j.cnki.rao.2017.0033]
点击复制

Nod样受体蛋白-3（NLRP3）炎症小体在翼状胬肉中的表达/HTML
分享到：

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年2期
页码:
125-128
栏目:
实验研究
出版日期:
2017-02-05

文章信息/Info

Title:
Expression of NOD-like receptor pyrin domain-3（NLRP3）inflammasome in pterygium
作者:
郭切; 李志远; 荆凡波
266003　山东省青岛市，青岛大学附属医院
Author(s):
GUO Qie; LI Zhi-Yuan; JING Fan-Bo
Affiliated Hospital of Qingdao University，Qing-dao 266003，Shandong Province，China
关键词:
翼状胬肉; Nod样受体蛋白-3; 半胱天冬酶-1; 白细胞介素-1β; 白细胞介素-18
Keywords:
pterygium; NOD-like receptor pyrin domain-3; caspase-1; IL-1b; IL-18
分类号:
R777.33
DOI:
10.13389/j.cnki.rao.2017.0033
文献标志码:
A
摘要:
目的　观察翼状胬肉组织和正常结膜组织中Nod样受体蛋白-3（Nod-like receptor pyrin domain3，NLRP3）炎症小体、细胞因子白细胞介素-1β（interleukin-1 beta，IL-1β）的表达，探讨NLRP3炎症小体在翼状胬肉发生过程中的作用。方法　选取20例翼状胬肉组织和6例正常结膜组织为实验对象，采用免疫组织化学法检测NLRP3、IL-1β蛋白的表达，通过Western-blot检测半胱天冬酶-1（cysteine-aspartic acid protease 1，Caspase-1）及Pro-caspase-1的蛋白表达水平，并通过实时荧光定量PCR检测白细胞介素-18（interleukin-18，IL-18）的基因表达水平。结果　NLRP3在正常结膜上皮细胞中无表达，而在翼状胬肉组织的基底部呈阳性表达；IL-1β在正常结膜组织中无表达，而在翼状胬肉组织中呈阳性表达；Pro-caspase-1在正常结膜组织和翼状胬肉中的表达差异无统计学意义（P>0.05），而其活性形式Caspase-1在翼状胬肉组织中的表达明显高于正常结膜组织（P<0.05）；IL-18在胬肉组织中的平均表达水平明显高于正常结膜组织（P<0.05）。结论　NLRP3信号通路活化后，其下游信号分子Caspase-1、IL-1β、IL-18在翼状在胬肉组织中异常高表达，说明NLRP3炎症小体及相关信号通路可能在翼状胬肉的进展过程中发挥作用。
Abstract:
Objective　To observe the expression of NOD-like receptor pyrin domain-3（NLRP3）inflammasome and interleukin-1 beta（IL-1b）in pterygium and normal conjunctiva specimens,and clarify the role of NLRP3 in the development of pterygium．Methods　Specimens from 20 cases of pterygium and 6 cases of normal eonjunctival were analyzed for establishing the expression of NLRP3 and IL-1b by immune-histochemistry.The level of caspase-1 and pro-caspase-1 protein were analyzed by Western-blot,gene expression of interleukin-18 (IL-18) was detected by RT-PCR.Results　NLRP3 was not expressed in normal conjunctival epithelial cells,but was positive in the basal part of pterygium;IL-1was not expressed in normal conjunctival tissues,but was positive in pterygium.There was no significant difference about the expression of Pro-caspase-1 in normal conjunctiva and pterygium (P>0.05),However,caspase-1,also known as the active form of pro-caspase-1 expressed in pterygium was higher than that in normal conjunctiva(P<0.05).The average level of IL-18 in 20 cases of pterygium was significantly higher than that in normal conjunctiva (P<0.05).Conclusion　After NLRP3 signaling pathway activating in pterygium tissue,caspase-1,IL-1β and IL-18 are high expression abnormally,suggest that NLRP3 inflammasome and the related signaling pathways may play a role in the progression of pterygium.

参考文献/References:

［1］　KAUP S.Comment on：Role of subconjunctival bevacizumab in primary pterygium surgery［J］.Indian J Ophthalmol，2016，64（8）：614-615.
［2］　ERDL H.Developments and current approaches in the treatment of pterygium［J］.Int Ophthalmol，2016，45（6）：564-569.
［3］　DESWAERTE V，RUWANPURA SM，JENKINS B.Transcriptional regulation of inflammasome-associated pattern recognition receptors，and the relevance to disease pathogenesis［J］.Mol Immunol，2016，32（7）：196-205.
［4］　SHAHZAD K，BOCK F，AL-DABET MM，GADI I，NAZIR S，WANG H，et al.Stabilization of endogenous Nrf2 by minocycline protects against Nlrp3-inflammasome induced diabetic nephropathy［J］.Sci Rep，2016，12（5）：345-349.
［5］　SAFI H，KHEIRKHAH A，MAHBOD M，MOLAEI S，HASHEMI H，JABBARVAND M.Correlations between histopathologic changes and clinical features in pterygia［J］.J Ophthalmic Vis Res，2016，11（2）：153-158.
［6］　SETHI P，BHATIA PK，GUPTA N，SINGH K.Multiple pterygium syndrome：Challenge for anesthesiologist［J］.Saudi J Anaesth，2016，10（3）：350-352.
［7］　LJUBOJEVIC′ V，GAJANIN R，AMIDZLC′ L，VUJKOVIC′ Z.The expression and significance of p53 protein and Ki-67 protein in pterygium［J］.Vojnosanit Pregl，2016，73（1）：16-20.
［8］　DIGENES M，DIGENES P，NETO CC，DUARTE FB，HOLANDA RR.Cutaneous manifestations associated with antiphospholipid antibodies［J］.Int J Dermatol，2014，43（9）：632-637.
［9］　ZOROQUIAIN P，JABBOUR S，ALDREES S，VILLA N，BRAVO-FILHO V，DIETRICH H，et al.High frequency of squamous intraepithelial neoplasia in pterygium related to low ultraviolet light exposure［J］.Saudi J Ophthalmol，2016，30（2）：113-116.
［10］　SHRIVASTAVA G，LEN-JUREZ M，GARCA-CORDERO J，MEZA-SNCHEZ DE，CEDILLO-BARRN L.Inflammasomes and its importance in viral infections［J］.Immunol Res，2016，34（7）：647-659.
［11］　NAKAMURA K，KAWAKAMI T，YAMAMOTOA N，TOMIZAWAA M，FUJIWARAB T，TOMONORI I，et al.Activation of the NLRP3 inflammasome by cellular labile iron［J］.Exp Hematol，2016，44（2）：116-124.
［12］　SUN S，XIA S，JI Y，KERSTEN S，QI L.The ATP-P2X7 signaling axis dispensable for obesity-associated inflammasome activtion in adipose tissue［J］.Diabetes，2012，61：1471-1478.
［13］　LAMKANFI M，DIXIT VM.Inflammasomes and their roles in health and disease［J］.Annu Rev Cell Dev Biol，2012，28：137-161.
［14］　ROCK KL，KATAOKA H，LAI JJ.Uric acid as a danger signal in gout and its comorbidities［J］.Nat Rev Rheumatol，2013，9：13-23.
［15］　MARTINON F，MAYOR A，TSCHOPP J.The inflammasomes：guardians of the body［J］.Annu Rev Immunol，2009，27：229-265.
［16］　SHAYEGAN MR，KHAKZAD MR.Evaluation of transforming growth factor-beta1 gene expression in pterygium tissue of atopic patients［J］.J Chin Med Assoc，2016，79（10）：565-569.
［17］　SAHIN M，UGAN Y.Potential role of interleukin-18 in patients with rheumatoid arthritis-associated carotid intima-media thickness but not insulin resistance［J］.Eur J Rheumatol，2014，1（4）：135-139.

相似文献/References:

[1]吴恺 刘二华 谭钢 邵毅.雷公藤多甙对体外培养的人翼状胬肉上皮鳞状化生的影响[J].眼科新进展,2012,32(6):000.
[2]叶芬 田农 薛春燕 施宇华 曹茜 黄振平.翼状胬肉面积与术后残余像差之间的关系[J].眼科新进展,2013,33(1):000.
[3]贾烨 赖兆光.翼状胬肉切除术式对术后眼表修复的影响[J].眼科新进展,2013,33(1):000.
[4]李新宇 王娟 徐玲娟 胡军 胡维琨 李贵刚.绷带型角膜接触镜在翼状胬肉手术中的应用[J].眼科新进展,2013,33(8):000.
[5]李景翠 张雪菲.组织因子在翼状胬肉中的表达[J].眼科新进展,2012,32(2):000.
[6]彭立 谢青 蔡枫 李爱国.同步辐射微束X射线检测翼状胬肉实验条件的探讨[J].眼科新进展,2013,33(11):000.
[7]石一宁,张纯涛.TGF-β1、TGF-βRⅡ及Smad4蛋白在原发性翼状胬肉中的表达[J].眼科新进展,2010,30(5):000.
[8]黄 焱 杨 静 张 娣 马惠杰 廖 凯沈 平 袁 安.自体角膜缘干细胞移植与常规翼状胬肉切除术疗效比较[J].眼科新进展,2009,29(1):000.
[9]范少君,陈元芝.细胞生长因子与翼状胬肉的关系研究新进展[J].眼科新进展,2014,34(5):494.[doi:10.13389/j.cnki.rao.2014.0136]
　FAN Shao-Jun,CHEN Yuan-Zhi.Relationship between cell growth factor and pterygium[J].Recent Advances in Ophthalmology,2014,34(2):494.[doi:10.13389/j.cnki.rao.2014.0136]
[10]张海江,霍鸣,董洁玉,等.核心蛋白多糖抑制翼状胬肉成纤维细胞增生的实验研究[J].眼科新进展,2014,34(8):725.[doi:10.13389/j.cnki.rao.2014.0198]
　ZHANG Hai-Jiang,HUO Ming,DONG Jie-Yu,et al.Inhibitive effects of Decorin on proliferation of cultured human pterygium fibroblasts[J].Recent Advances in Ophthalmology,2014,34(2):725.[doi:10.13389/j.cnki.rao.2014.0198]

更新日期/Last Update: 2017-03-01