[1]刘大鹏,张耀东,熊虹,等.生长发育迟滞与鼠视网膜病变发生的相关研究[J].眼科新进展,2016,36(12):1116-1120.[doi:10.13389/j.cnki.rao.2016.0298]
　LIU Da-Peng,ZHANG Yao-Dong,XIONG Hong,et al.Correlation of intra-, extrauterine malnutrition and retinopathy of prematurity in mice[J].Recent Advances in Ophthalmology,2016,36(12):1116-1120.[doi:10.13389/j.cnki.rao.2016.0298]
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生长发育迟滞与鼠视网膜病变发生的相关研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年12期
页码:
1116-1120
栏目:
实验研究
出版日期:
2016-12-05

文章信息/Info

Title:
Correlation of intra-, extrauterine malnutrition and retinopathy of prematurity in mice
作者:
刘大鹏; 张耀东; 熊虹; 孟莉; 康文清; 孙慧清; 许邦礼; 王彩君; 李明超; 金娟; 郭静; 孙仙桃; 周炎娟; 余增渊
450018　河南省郑州市，郑州市儿童医院NICU（刘大鹏，张耀东，熊虹，康文清，许邦礼，王彩君，金娟，郭静，周炎娟）； 453003　河南省新乡市，新乡医学院基础医学院（孟莉）；450018　河南省郑州市，郑州市儿童医院早产儿病区（孙慧清，李明超，余增渊）；450018　河南省郑州市，郑州市儿童医院眼科（孙仙桃）
Author(s):
LIU Da-Peng; ZHANG Yao-Dong; XIONG Hong; MENG Li; KANG Wen-Qing; SUN Hui-Qing; XU Bang-Li; WANG Cai-Jun; LI Ming-Chao; JIN Juan; GUO Jing; SUN Xian-Tao; ZHOU Yan-Juan; YU Zeng-Yuan
关键词:
生长发育迟滞; 早产儿视网膜病变; 胰岛素样生长因子-1; 血管内皮生长因子; 精氨酸2
Keywords:
growth retardation;  retinopathy of prematurity;  insulin-like growth factors-l ; vascular endothelial growth factor ;  arginine 2
分类号:
Ｒ７７４.１
DOI:
10.13389/j.cnki.rao.2016.0298
文献标志码:
A
摘要:
目的　探讨生后生长发育迟滞对鼠视网膜的影响。方法　将怀孕１８ｄ的Ｃ５７ＢＬ／６母鼠随机分为正常对照组、生长发育迟滞组、氧诱导视网膜病（ｏｘｙｇｅｎ-ｉｎｄｕｃｅｄｒｅｔｉｎｏｐａｔｈｙ，ＯＩＲ）组、生长发育迟滞＋ＯＩＲ组。正常对照组母鼠予正常饮食；生长发育迟滞组母鼠予等热卡低蛋白饮食，持续至幼鼠出生２１ｄ；ＯＩＲ组建立ＯＩＲ模型；生长发育迟滞＋ＯＩＲ组予生长发育迟滞及ＯＩＲ联合诱导。于出生１４ｄ、２１ｄ检测血糖、胰岛素和胰岛素样生长因子-１（ｉｎｓｕｌｉｎ-ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ，ＩＧＦ-１）的变化。于生后１４ｄ、２１ｄ留取视网膜组织，检测精氨酸２活性及血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＶＥＧＦ）的表达。出生７０ｄ，应用视网膜电流图评估视网膜功能。结果　生长发育迟滞组、生长发育迟滞＋ＯＩＲ组小鼠出生体质量明显低于其他两组（均为Ｐ＜０．０５），生后１４ｄ、２１ｄ，生长发育迟滞组、生长发育迟滞＋ＯＩＲ组小鼠体质量、胰岛素、ＩＧＦ-１值、ＶＥＧＦ在视网膜表达均显著低于正常对照组（均为Ｐ＜０．０５），而精氨酸２活性显著高于正常对照组（均为Ｐ＜０．０５）。ＯＩＲ组、生长发育迟滞组、生长发育迟滞＋ＯＩＲ组小鼠视网膜毛细血管密度指数增加，而以生长发育迟滞＋ＯＩＲ组小鼠增加最显著。生后７０ｄ时ＯＩＲ组、生长发育迟滞组小鼠视网膜电流图反应减弱，ＲｍＰ２振幅（双极细胞）及８Ｈｚ频闪振幅（Ｒ８：视网膜内功能）显著减小，且生长发育迟滞＋ＯＩＲ组小鼠变化更加明显。结论　生长发育迟滞小鼠影响ＩＧＦ-１、ＶＥＧＦ的表达及精氨酸２的活性，对视网膜病的发生有显著促进作用。
Abstract:
Objective To investigate the effects of growth retardation after preterm birth on retinopathy of prematurity. Methods The pregnant 18 days C57BL / 6 mice were randomly divided into normal control group,growth retardation group,oxygen-induced retinopathy ( OIR) group,and growth retardation + OIR group. The pregnant mice in normal control group were only given the normal diet. The pregnant mice in growth retardation group were given the isocaloric low-protein diet,continued t0 21 days for pups after birth,to induce growth retardation pups. For OIR group pups,the OIR models were established in the mother and pups. The pregnant mice in growth retardation + OIR group were grven growth retardation and OIR models were established.At 14 days and 21 days after birth,the blood glucose,insulin,and insulin-like growth factor-l (IGF-1) were tested. The arguune 2 and vascular endothelial growth factor ( VEGF) in retinal tissue were detected. At 70 days after birth,electroretinogram (ERG) was used to assess retinal function. Results The birth weight of the growth retardation group and growth retardation + OIR group were sigruficantly lower than those of other two groups ( all P < 0. 05 ) ,at 14 days and 21 days after birth,the weight,insulin,IGF-l and VEGF in the growth retardation group and growth retardation + OIR group were sigruficantly lower than those of the normal control group ( all P < 0. 05 ) ,but argirune 2 was much higher ( all P < 0. 05 ) . The retinal capillary density index significantly increased in OIR group,growth retardation group and growth retardation + OIR group,especially in growth retardation +OIR group. At 70 days of mice after birth,ERG analysis was done for all groups,ERG response weakened in growth retardation group and OIR group,and RmP2 amplitude ( bipolar cells) and 8 Hz flicker amplitude ( R8 :the retina function) was significantly reduced. ERG response was the most weakest in growth retardation + OIR groups. Conclusion Growth retardation after birth for prematurity can effect the expression of IGF-1 ,VEGF and arginine 2 ,and significantly effect the occurrence of retinopathy of prematurity.

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备注/Memo

备注/Memo:
河南省医学科技攻关计划项目（编号：２０１４０３２６０、２０１２０４１４０）；河南省基础与前沿技术研究计划项目（编号： １１２３００４１０１６４）；河南省教育厅自然科学研究项目（编号：２０１１Ｂ３１０００９）；郑州市科技攻关项目（编号：２０１５０１５３）
更新日期/Last Update: 2016-12-19