[1]武朋朋,高晓唯,郭继华,等.基质蛋白Fibulin-5在眼眶IgG4相关性疾病中的表达[J].眼科新进展,2016,36(12):1121-1124.[doi:10.13389/j.cnki.rao.2016.0299]
　WU Peng-Peng,GAO Xiao-Wei,GUO Ji-Hua,et al.Expression of matrix protein Fibulin-5 in orbital IgG4-related ocular diseases[J].Recent Advances in Ophthalmology,2016,36(12):1121-1124.[doi:10.13389/j.cnki.rao.2016.0299]
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基质蛋白Fibulin-5在眼眶IgG4相关性疾病中的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年12期
页码:
1121-1124
栏目:
实验研究
出版日期:
2016-12-05

文章信息/Info

Title:
Expression of matrix protein Fibulin-5 in orbital IgG4-related ocular diseases
作者:
武朋朋; 高晓唯; 郭继华; 蔡岩; 高雯; 田艳明
832003　新疆维吾尔自治区石河子市，石河子大学医学院（武朋朋，田艳明）；830013　新疆维吾尔自治区乌鲁木齐市，解放军第474医院全军眼科中心（高晓唯，郭继华，蔡岩，高雯，田艳明）
Author(s):
WU Peng-Peng; GAO Xiao-Wei; GUO Ji-Hua; CAI Yan; GAO Wen; TIAN Yan-Ming
Shihezi University School of Medicine( WU Peng-Peng,TIAN Yan-Ming ) , Shihezi 832002, Xinjiang Uygur Autonomous Region, China; Ophthalmic Center,the 474th Hospital of Chinese PLA ( GAO Xiao-Wei,GUO Ji-Hua, CAI Yan, GAO Wen, TIAN Yan-Ming ) , Urumqi 830013 , Xinjiang Uygur Autonomous Region,China
关键词:
Fibulin-5; IgG4-ROD; 纤维化; 泪腺
Keywords:
Fibulin-5 ; IgG4-ROD ; fibrosis ; lacrimal gland
分类号:
Ｒ７７７.５
DOI:
10.13389/j.cnki.rao.2016.0299
文献标志码:
A
摘要:
目的　探讨基质蛋白Ｆｉｂｕｌｉｎ-５与眼眶ＩｇＧ４相关性疾病（ＩｇＧ４-ｒｅｌａｔｅｄｏｃｕｌａｒｄｉｓ-ｅａｓｅ，ＩｇＧ４-ＲＯＤ）纤维化、炎症、硬化的关系。方法　收集２０例（２０眼）眼眶开放性粉碎性骨折清创术中采集的正常泪腺组织标本作为对照组，２０例（２０眼）眼眶ＩｇＧ４-ＲＯＤ患者的泪腺肿物作为ＩｇＧ４-ＲＯＤ组，采用免疫组织化学染色结合图像分析法对基质蛋白Ｆｉｂｕｌｉｎ-５进行半定量分析，采用实时荧光定量ＰＣＲ和Ｗｅｓｔｅｒｎ-ｂｌｏｔｔｉｎｇ法检测Ｆｉｂｕｌｉｎ-５ｍＲＮＡ及蛋白的相对表达量。结果　ＩｇＧ４-ＲＯＤ组中Ｆｉｂｕｌｉｎ-５ｍＲＮＡ的表达水平为３．６９±１．７２，显著高于对照组（１．６９±０．４５），两组比较差异有统计学意义（Ｐ＜０．０５）。Ｆｉｂｕｌｉｎ-５蛋白与Ｆｉｂ-ｕｌｉｎ-５ｍＲＮＡ表达变化趋势较为一致。ＩｇＧ４-ＲＯＤ组中Ｆｉｂｕｌｉｎ-５蛋白的表达水平为０．５５±０．１２，对照组中Ｆｉｂｕｌｉｎ-５蛋白表达水平为０．１９±０．１１，两组比较差异有统计学意义（Ｐ＜０．０１）。免疫组织化学结果显示，ＩｇＧ４-ＲＯＤ组中泪腺细胞排列不规则，染色为黄色、棕黄色的纤维化细胞排列紊乱，对照组中泪腺腺管规则，细胞规则排列，核染蓝色。ＩｇＧ４-ＲＯＤ组Ｆｉｂｕｌｉｎ-５蛋白的阳性表达显著高于对照组。结论　ＩｇＧ４-ＲＯＤ组中Ｆｉｂｕｌｉｎ-５ｍＲＮＡ和蛋白表达较对照组显著升高，Ｆｉｂｕｌｉｎ-５可能促进眼眶ＩｇＧ４-ＲＯＤ的纤维化及硬化。
Abstract:
Objective To discuss the relation of matrix protein Fibulin-5 and fibrosis,inflammation, sclerosis in orbital IgG4-related ocular disease ( IgG4-ROD). Methods Twenty cases (20 eyes) with open fracture debridement were collected as the control group,the normal lacrimal gland tissues were harvested,and 20 cases ( 20 eyes) with IgG4-ROD were also collected as IgG4-ROD group,the lacrimal gland tumor tissues were harvested. The immunohistochemical stairung and image analysis were performed to semi-quantitative analyze Fibulin-5 ,and Fibulin-5 mRNA and protein expression were detected by real-time quantitative PCR and Western blot. Results The expression level of Fibulin-5 mRNA in IgG4-ROD group was 3. 69 + 1. 72 ,sigruficantly higher than the normal group ( 1. 69 +0. 45 ) ,and the difference was statistically significant ( P < 0. 05) . Fibulin-5 protein and Fibulin-5 mRNA expression trends was more consistent. The expression of Fibulin-5 protein in IgG4-ROD group was 0. 55 + 0. 12,control group was 0. 19 + 0. 11 ,and the difference was statistically sigruficant ( P < 0. 01 ) . Inuuunohistochemistry showed that the lacrimal gland cells in IgG4-ROD group arranged in irregular,dyed yellow,brown fibrosis cells were disorder ; The lacrimal duct in the control group was regularly,cell nuclei arranged regularly and blue stained. The expression of Fibulin-5 protein in IgG4-ROD group was significantly higher than that in the control group. Conclusion Fibulin-5 mRNA and protein expression in the IgG4-ROD group are sigruficantly higher than those in the control group,and Fibulin-5 may promote the fibrosis and sclerosis of orbital IgG4-ROD.
更新日期/Last Update: 2016-12-19