[1]孔蕾,胡敏,华启云,等.实验性视网膜缺血-再灌注损伤中坏死性凋亡相关因子的表达变化[J].眼科新进展,2016,36(12):1109-1112.[doi:10.13389/j.cnki.rao.2016.0296]
　KONG Lei,HU Min,HUA Qi-Yun,et al.Expression changes of necroptosis related factors in experimental retinal ischemia reperfusion injury[J].Recent Advances in Ophthalmology,2016,36(12):1109-1112.[doi:10.13389/j.cnki.rao.2016.0296]
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实验性视网膜缺血-再灌注损伤中坏死性凋亡相关因子的表达变化
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年12期
页码:
1109-1112
栏目:
实验研究
出版日期:
2016-12-05

文章信息/Info

Title:
Expression changes of necroptosis related factors in experimental retinal ischemia reperfusion injury
作者:
孔蕾; 胡敏; 华启云; 张红
650031　云南省昆明市，云南省第二人民医院眼科
Author(s):
KONG Lei; HU Min; HUA Qi-Yun; ZHANG Hong
Department of Ophthalmology,the Second People’s Hosptial of Yunnan, Province, Kunming 650031, Yunnan, Province , China
关键词:
坏死性凋亡; 视网膜缺血-再灌注损伤; 受体相互作用蛋白; 鼠
Keywords:
necroptosis;  retinal ischemia reperfusion injury;  receptor interacting protein ;  rat
分类号:
Ｒ７７４.１
DOI:
10.13389/j.cnki.rao.2016.0296
文献标志码:
A
摘要:
目的　建立视网膜缺血-再灌注损伤（ｒｅｔｉｎａｌｉｓｃｈｅｍｉａｒｅｐｅｒｆｕｓｉｏｎｉｎｊｕｒｙ，ＲＩＲＩ）模型，观察坏死性凋亡是否参与其病理过程。方法　野生型Ｃ５７小鼠随机分为对照组及实验组。对照组不做任何处理，实验组采用前房灌注法建立ＲＩＲＩ模型，并于ＲＩＲＩ后１ｄ、２ｄ、４ｄ、７ｄ分别收集视网膜或眼球，行荧光定量ＰＣＲ、Ｗｅｓｔｅｒｎｂｌｏｔ、免疫荧光染色检测受体相互作用蛋白（ｒｅｃｅｐｔｏｒｉｎｔｅｒａｃｔｉｎｇｐｒｏｔｅｉｎ，ＲＩＰ）３及ＲＩＰ１、白细胞介素-１β、白细胞介素-６、肿瘤坏死因子-α、Ｃａｓｐａｓｅ８的表达变化。结果　荧光定量ＰＣＲ检测ＲＩＰ３、ＲＩＰ１、白细胞介素-１β、白细胞介素-６、肿瘤坏死因子-α、Ｃａｓｐａｓｅ８的ｍＲＮＡ表达，与对照组比较，实验组在不同时间点均有显著升高，差异均有统计学意义（均为Ｐ＜０．００１）。ＲＩＰ３及ＲＩＰ１表达以早期升高为主，后期逐渐下降。Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ检测发现ＲＩＰ３在ＲＩＲＩ后１ｄ及２ｄ显著升高，随后呈下降趋势。组织切片免疫荧光染色也发现ＲＩＰ３在ＲＩＲＩ后１ｄ及２ｄ显著升高，随后呈下降趋势。结论　实验性ＲＩＲＩ模型中，坏死性凋亡参与了其病理过程。坏死性凋亡特异性蛋白ＲＩＰ３表达以早期升高为主，后期逐渐下降。
Abstract:
Objective To observe whether necroptosis involved in the pathological process of retinal ischemia reperfusion injury ( RIRI) or not by establislung an retinalischemia reperfusion injury model. Methods Wild type C57 mice were divided into control group and experimental group randomly. The mice in control group were treated with nothing. RIRI models in experimental group were induced with anterior chamber perfusion. The retina or eyeballs were harvested,Q-PCR,Western blot and immunofluorescence stauung were performed to test receptor interacting protein 3 (RIP3),RIPl,IL-1[3,TNF-a,Caspase-8,and IL-6 changes at l day,2 days,4 days,7 days. Results The mRNA expression change of RIP3 ,RIPl,IL-1[3,TNF-a,Caspase-8 , and IL-6 were detected by Q-PCR. Compared with control group,all genes mRNA expression were increased significantly at different time points ( all P < 0. 001) . RIP3 ,RIP1 mRNA expressions were increased at the early phase of RIRI,and decreased in the late phase slowly. Western blot found RIP3 expression increased dramatically at l day and 2 days,then decreased gradually. Retinal immunofluorescence stanung also found RIP3 expression increased dramatically at l day and 2 days,then decreased slowly. Conclusion Necroptosis participates in the pathological process of experimental RIRI. As a specified marker of necroptosis,RIP3 expression increase at the early phase of RIRI, and decrease at the later phase gradually.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：８１５６０１６８）
更新日期/Last Update: 2016-12-19