[1]赵昆,黄敏丽,王淼,等.雷帕霉素对视网膜缺血-再灌注损伤的保护作用[J].眼科新进展,2014,34(5):409-413.[doi:10.13389/j.cnki.rao.2014.0113]
　ZHAO Kun,HUANG Min-Li,WANG Miao,et al.Protective effects of raamycin on retinal is-chemia-reperfusion injury of rats[J].Recent Advances in Ophthalmology,2014,34(5):409-413.[doi:10.13389/j.cnki.rao.2014.0113]
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雷帕霉素对视网膜缺血-再灌注损伤的保护作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
34卷
期数:
2014年5期
页码:
409-413
栏目:
实验研究
出版日期:
2014-05-05

文章信息/Info

Title:
Protective effects of raamycin on retinal is-chemia-reperfusion injury of rats
作者:
赵昆; 黄敏丽; 王淼; 刘欣
５３００２１　广西壮族自治区南宁市，广西医科大学第一附属医院眼科
Author(s):
ZHAO Kun;  HUANG Min-Li;  WANG Miao ;  LIU Xin
关键词:
雷帕霉素; Ｗｉｓｔａｒ大鼠; 缺氧诱导因子１α; 视网膜缺血-再灌注损伤; 细胞凋亡
Keywords:
rapamycin ;  wistar rat ;  hypoxia-inducible factor I eL ;  retinal ischemia-reperfusion injury ;  cell apoptosis
DOI:
10.13389/j.cnki.rao.2014.0113
文献标志码:
A
摘要:
目的　探讨雷帕霉素对视网膜缺血-再灌注损伤（ｒｅｔｉｎａｌｉｓｃｈｅｍｉａ-ｒｅｐｅｒｆｕｓｉｏｎｉｎｉｕｒｙ，ＲＩＲＩ）的保护作用和机制。方法　７５只ＳＰＦ级健康雄性Ｗｉｓｔａｒ大鼠随机分为３组：空白对照组、实验对照组、实验组，每组２５只。实验对照组和实验组行前房灌注建立ＲＩＲＩ模型。实验组在前房灌注前２ｈ按２ｍｇ?ｋｇ－１剂量腹腔注射雷帕霉素，实验对照组大鼠腹腔注射等量的生理盐水和ＤＭＳＯ。分别在再灌注后０ｈ、６ｈ、１２ｈ、２４ｈ、４８ｈ取３组视网膜标本，ＨＥ染色法观察视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｏｎ ｃｅｌｌｓ，ＲＧＣ）形态及测量视网膜内层厚度；ＴＵＮＥＬ法检测视网膜组织中凋亡细胞的表达；Ｒｅａｌ-ｔｉｍｅＰＣＲ检测缺氧诱导因子１α （ｈｙｐｏｘｉａ-ｉｎｄｕｃｉｂｌｅｆａｃｔｏｒ１α，ＨＩＦ-１α）ｍＲＮＡ在视网膜组织的表达水平。结果　再灌注后０ｈ，实验组视网膜内层厚度为（１３７５５±７７６）μｍ，视网膜水肿较实验对照组轻，实验对照组视网膜内层厚度为（１６２２６±６４１）μｍ，且实验组ＲＧＣ空泡化现象较少；再灌注６ｈ以后实验组视网膜内层厚度均较实验对照组厚（均为Ｐ＜０．０５）。实验组再灌注后１２ｈ、２４ｈ、４８ｈ的细胞凋亡情况明显低于实验对照组（均为Ｐ＜０．０５）。再灌注后０ｈ、６ｈ、１２ｈ、２４ｈ、４８ｈ实验组视网膜组织中ＨＩＦ-１αｍＲＮＡ表达水平较实验对照组降低（均为Ｐ＜０．０５）。结论　雷帕霉素对ＲＩＲＩ具有保护作用，ＨＩＦ-１α表达水平的下调可能与该作用有关。
Abstract:
Objective To study the protective effects and its mechanism of rapamycin ( RAPA) on retinal ischemia-reperfusion injury ( RIRI) of rat. Methods Seventy-five SPF healthy male rats were random divided into three groups: blank control group , experimental control group and experimental group , 25 rats in each group. RIRI models were induced in experimental control group and experimental group by increasing intraocular pressure via an intracameral catheter. The rats in experimental group underwent the intraperitoneal injection with PAPA (2 mg . kg ’1 ) 2 hours before retinal is-chemia. and the experimental control group with normal sodium and DMSO. The retinal specimens of three groups were taken at o hour,6 hours. 12 hours.24 hours and 48 hours after reperfusion. The morphology of retinal ganglion cells ( RGC) and inner retinal layer thickness were observed or measured by HE staining,TUNEL technique was used to examine the apoptosis of RGC , and the level of hypoxia-inducible factor le/ mR-NA in retinal tissue was analyzed by real-time PCR assay. Results At o hour after reperfusion, the inner retinal layer thickness in experimental group and experimental control group were ( 137. 55 +7. 76 ) Vm and ( 162. 26 + 6. 41) Vm , the retinal edema in experimental group was less than that in experimental control group , and the vacuolar phenomenon of RGC was also less. At 6 hours later after reperfusion , the inner retinal layer thickness of experimental group were greater than that of experimental control group ( all P < 0. 05 ) . At 12 hours ,24 hours and 48 hours after reperfusion , the apoptotic cells in experimental group were obvious less than those in experimental control group ( all P < 0. 05 ). The expressions of hypoxia-inducible factor le/ mRNA at o hour.6 hours . 12 hours ,24 hours and 48 hours after reperfusion in experimental group were obvious lower than those in experimental control group ( all P < 0. 05 ) . Conclusion RAPA can protect the retina against RIRI. and the down-regulation of hypoxia-inducible factor la may be involved in the mechanism of the protection.

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备注/Memo

备注/Memo:
广西自然科学基金资助（编号：２０１０ＧＸＮＳＦＡ０１３１４０）
更新日期/Last Update: 2014-05-04