[1]谢兵,李中文,周远忠.中药提取物姜黄素对人视网膜神经胶质瘤细胞的放射增敏作用[J].眼科新进展,2016,36(5):431-434.[doi:10.13389/j.cnki.rao.2016.0115]
　XIE Bing,LI Zhong-Wen,ZHOU Yuan-Zhong.Radiosensitization effects of curcumin on human retinal glioma cells[J].Recent Advances in Ophthalmology,2016,36(5):431-434.[doi:10.13389/j.cnki.rao.2016.0115]
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中药提取物姜黄素对人视网膜神经胶质瘤细胞的放射增敏作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年5期
页码:
431-434
栏目:
实验研究
出版日期:
2016-05-05

文章信息/Info

Title:
Radiosensitization effects of curcumin on human retinal glioma cells
作者:
谢兵; 李中文; 周远忠
563003　贵州省遵义市，遵义医学院附属医院眼科（谢兵）；563003　贵州省遵义市，遵义医学院附属医院肿瘤医院（李中文）；563003　贵州省遵义市，遵义医学院预防医学教研室（周远忠）
Author(s):
XIE Bing ;  LI Zhong-Wen ; ZHOU Yuan-Zhong
Department of Ophthalmology, the Affiliated Hospital of Zunyi Medical College ( XIE Bing ) , Zunyi 563003 . Guizhou Province , China ; Tumor Hospital of the Affiliated Hospital of Zunyi Medical College ( LI Zhong-Wen ) , Zunyi 563003 , Guizhou Province . China ; Prevention Medical Research Room of Zunyi Medical College ( ZHOU Yuan-Zhong ) , Zunyi 563003 , Guizhou Province , China
关键词:
姜黄素; 放射增敏; 神经胶质瘤; 细胞周期; 细胞凋亡
Keywords:
curcumin ;  radiosensitization ;  glioma ;  cell cycle ;  apoptosis
DOI:
10.13389/j.cnki.rao.2016.0115
文献标志码:
A
摘要:
目的　研究中药提取物姜黄素对人视网膜神经胶质瘤ＷＥＲＩ-Ｒｂ-１细胞的放射增敏作用。方法　采用Ｘ射线辐照仪辐照人视网膜神经胶质瘤ＷＥＲＩ-Ｒｂ-１细胞。ＣＣＫ-８细胞活性检测试剂盒检测０μｍｏｌ·Ｌ－１、１μｍｏｌ·Ｌ－１、５μｍｏｌ·Ｌ－１、１０μｍｏｌ·Ｌ－１、２０μｍｏｌ·Ｌ－１、３０μｍｏｌ·Ｌ－１姜黄素，０Ｇｙ、１Ｇｙ、２Ｇｙ、４Ｇｙ、８Ｇｙ射线以及姜黄素联合射线作用细胞不同时间对细胞活性的影响；流式细胞仪检测单独姜黄素、射线、姜黄素联合射线引起的细胞周期阻滞以及线粒体膜电位的变化情况；Ｈｏｃｈｅｓｔ３３２５８细胞核染色观察单独姜黄素、射线、姜黄素联合射线对细胞凋亡的影响。结果　０μｍｏｌ·Ｌ－１、１μｍｏｌ·Ｌ－１、５μｍｏｌ·Ｌ－１、１０μｍｏｌ·Ｌ－１、２０μｍｏｌ·Ｌ－１、３０μｍｏｌ·Ｌ－１姜黄素和单独的０Ｇｙ、１Ｇｙ、２Ｇｙ、４Ｇｙ、８Ｇｙ射线随剂量和时间增加均能引起细胞活性的下降；２Ｇｙ射线联合不同浓度的姜黄素（０μｍｏｌ·Ｌ－１、１μｍｏｌ·Ｌ－１、５μｍｏｌ·Ｌ－１、１０μｍｏｌ·Ｌ－１、２０μｍｏｌ·Ｌ－１、３０μｍｏｌ·Ｌ－１）相比单独的姜黄素可以引起细胞活性显著下降；１０μｍｏｌ·Ｌ－１姜黄素联合２Ｇｙ射线能够将细胞周期明显阻滞在Ｇ２／Ｍ期，阻滞率为（７９．６±２．１）％，并诱导细胞凋亡和线粒体膜电位下降为（４８．３±４．３）％。结论　姜黄素联合射线对人视网膜神经胶质瘤ＷＥＲＩ-Ｒｂ-１细胞具有显著的放射增敏作用，并诱导细胞凋亡和线粒体膜电位下降，为姜黄素的临床应用提供一定理论依据。
Abstract:
Objective To investigate the radiosensitization effects of curcunun on human retinal glioma WERI-Rb-I cells. Methods Cell activity detection kit ( CCK-8) was used to detect the cell viability induced by curcumin (0 ymol . L-’,1 ymol . 1’1 ,5 Vmol . L-l , 10 ymol . 1’1 ,20 ymol . L-’,30 Vmol . L-’) , irradiation (0 Gy,l Gy,4 Gy,8 Gy) and curcunun combined with irradiation, flow cytometry was used to test the cell cycle, and mitochondrial membrane potential and Hochest33258 staining was used to detect the cell apoptosis induced by curcumin, irradiation and curcunun combined with irradiation. Results Curcumin (0 pmol . 1’1 ,1 pdmol . 1’1 ,5 pmol . 1’1 ,10 pdmol . 1-1 ,20 pdmol . 1’1 ,30 ymol . L-’) and irradiation (0 Gy,l Gy,4 Gy,8 Gy) could decrease cell viability with dose and time dependence. Curcumin (0 Vmol . 1’1 ,1 Vmol . L",5 Vmol . 1’1 ,10 ymol . 1’1 ,20 ymol . L-’,30 Vmol . L-’) combined with 2 Gy irradiation could more significant decrease cell viability than curcunun alone. In addition , 10 ymol . L -l curcumin combined with 2 Gy irradiation could arrest cell cycle at G2/M stage, the arrest rate was ( 79. 6 + 2. I ) % .induce cell apoptosis and mitochondrial membrane potential decrease to ( 48. 3 + 4. 3 ) % . Conclusion Curcumin combined with irradiation have sigruficant radiosensitization effects on WERI-Rb-I cells .and induce cell apoptosis and mitochondrial membrane potential decrease. These results may provide theoretical foundation for clinical application of curcumrn.

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更新日期/Last Update: 2016-05-04