[1]高文,陈陆霞,何彦津,等.姜黄素对人脉络膜黑色素瘤细胞株血管形成拟态抑制作用及其相关机制的研究[J].眼科新进展,2017,37(11):1018-1021.[doi:10.13389/j.cnki.rao.2017.0258]
　GAO Wen,CHEN Lu-Xia,HE Yan-Jin,et al.Inhibitory effects of curcumin on vasculogenic mimicry in choroid melanoma cell line OCM-1 and its mechanisms[J].Recent Advances in Ophthalmology,2017,37(11):1018-1021.[doi:10.13389/j.cnki.rao.2017.0258]
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姜黄素对人脉络膜黑色素瘤细胞株血管形成拟态抑制作用及其相关机制的研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年11期
页码:
1018-1021
栏目:
实验研究
出版日期:
2017-11-05

文章信息/Info

Title:
Inhibitory effects of curcumin on vasculogenic mimicry in choroid melanoma cell line OCM-1 and its mechanisms
作者:
高文; 陈陆霞; 何彦津; 梁凤鸣
300193　天津市，天津中医药大学第一附属医院眼科（高文，梁凤鸣）；300000　天津市，天津市眼科医院（陈陆霞）；300000　天津市，天津医科大学眼科医院（何彦津）
Author(s):
GAO Wen; CHEN Lu-Xia; HE Yan-Jin; LIANG Feng-Ming
Department of Ophthalmology，the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine（GAO Wen，LIANG Feng-Ming），Tianjin 300193，China；Tianjin Eye Hospital（CHEN Lu-Xia），Tianjin 300000，China；Eye Hospital of Tianjin Medical University（HE Yan-Jin），Tianjin 300000，China
关键词:
脉络膜黑色素瘤; 姜黄素; 血管形成拟态; PI3K
Keywords:
Choroid melanoma; curcumin; vasculogenic mimicry; PI3K
分类号:
R773
DOI:
10.13389/j.cnki.rao.2017.0258
文献标志码:
A
摘要:
目的　研究姜黄素对人脉络膜黑色素瘤细胞株OCM-1血管形成拟态（vasculogenic mimicry，VM）及其PI3K信号通路的影响。方法　三维培养人脉络膜黑色素瘤细胞株OCM-1，观察其形成VM的能力。观察梯度浓度（0 μmol·L-1、10 μmol·L-1、20 μmol·L-1、30 μmol·L-1、40 μmol·L-1）的姜黄素对OCM-1细胞株VM形成的影响，采用免疫细胞化学检测参与VM形成的PI3K与EPHA2的表达。结果　姜黄素在体外可以抑制OCM-1细胞株VM的形成，呈浓度依赖性。姜黄素对OCM-1细胞EphA2和PI3K蛋白表达的影响：EphA2和PI3K蛋白表达随姜黄素（10 μmol·L-1、20 μmol·L-1、30 μmol·L-1、40 μmol·L-1）浓度的升高呈浓度依赖性降低。各组分别与0 μmol·L-1 姜黄素组比较，差异均有统计学意义（均为P=0.000），其中30 μmol·L-1姜黄素组和40 μmol·L-1姜黄素组比较，差异无统计学意义（P=0.300、P=0.188）。结论　姜黄素能明显抑制OCM-1细胞株VM的形成，其机制可能与下调EPHA2、PI3K蛋白的表达，从而抑制PI3K信号通路有关。
Abstract:
Objective　To observe the effects of curcumin (Cur) on vasculogenic mimicry (VM) and PI3K pathway in choroid melanoma cell line OCM-1 in vitro 3-dimentional culture.Methods　Three-dimensional culture of OCM-1 cells was performed for the observation of VM formation and the influence of concentration gradient (0 μmol·L-1，10 μmol·L-1，20 μmol·L-1，30 μmol·L-1，40 μmol·L-1) Cur on VM，and immunohis to chemistry staining was used to detect the expression of PI3K and EphA2 in OCM-1 cell line.Results　Cur could markedly inhibit the formation of VM in choroid melanoma cell line OCM-1 in vitro 3-dimensional culture with a dose-dependent manner.Cur suppressed EphA2 and PI3K expression in a concentration-dependent manner，and the difference between groups with 0 μmol·L-1 Cur group was significant statistically (all P=0.000)，but there was no significant difference between 30 μmol·L-1 and 40 μmol·L-1 group (EphA2，P=0.300；PI3K，P=0.188).Conclusion　Cur can significantly inhibit VM formation in choroid melanoma cell line OCM-1，of which the mechanism may be related to the downregulation expression of EphA2 and PI3K，thus inhibiting PI3K signaling pathway.

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更新日期/Last Update: 2017-11-01