[1]曾官鹏,严丽英,徐双,等.MFN1在透镜诱导豚鼠近视模型视网膜上的表达及其意义[J].眼科新进展,2016,36(1):001-3.[doi:10.13389/j.cnki.rao.2016.0001]
　ZENG Guan-Peng,YAN Li-Ying,XU Shuang,et al.Expression and significance of MFNl in retina of guinea pig with lens induced myopia[J].Recent Advances in Ophthalmology,2016,36(1):001-3.[doi:10.13389/j.cnki.rao.2016.0001]
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MFN1在透镜诱导豚鼠近视模型视网膜上的表达及其意义
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年1期
页码:
001-3
栏目:
实验研究
出版日期:
2016-01-05

文章信息/Info

Title:
Expression and significance of MFNl in retina of guinea pig with lens induced myopia
作者:
曾官鹏; 严丽英; 徐双; 邹云春
637000　四川省南充市，川北医学院附属医院眼科，川北医学院眼视光学系
Author(s):
ZENG Guan-Peng ;  YAN Li-Ying; XU Shuang ; ZOU Yun-Chun
Department of Ophthalmology, North Sichuan Medical College , Nanchong 637000 , Sichuan Province , China
关键词:
透镜诱导型近视; 视网膜; 线粒体融合蛋白1
Keywords:
lens-induced myopia ;  retina ;  mitochondrial fusion protein 1
DOI:
10.13389/j.cnki.rao.2016.0001
文献标志码:
A
摘要:
目的　建立豚鼠光学离焦近视动物模型，初步探索线粒体融合蛋白１（ｍｉｔｏｃｈｏｎｄｒｉａｌｆｕｓｉｏｎｐｒｏｔｅｉｎ１，ＭＦＮ１）在光学离焦豚鼠近视动物模型视网膜上的表达。方法　给１５只４周龄幼年豚鼠单眼配戴－７．００Ｄ凹透镜，制作光学离焦性豚鼠近视模型，戴透镜眼作为实验眼，另一眼为自身对照眼。戴镜前及戴镜后１周、２周、３周分别进行检影验光和眼轴长度检查，同时于各时间点分别随机处死５只豚鼠，采用免疫组织化学染色检测视网膜中ＭＦＮ１的表达，双眼眼球参数检查结果比较采用ｔ检验，对免疫组织化学检查结果做描述定性分析。结果　屈光度：豚鼠戴镜前屈光状态均为远视状态，实验眼和对照眼屈光度差异无统计学意义（Ｐ＞０．０５）；用－７．００Ｄ凹透镜经１周、２周、３周光学离焦可以造成豚鼠远视屈光度数逐渐下降，戴镜后１周两组比较差异无统计学意义（Ｐ＝０．３８０）；戴镜后２周及３周实验眼与对照眼比较，差异均有统计学意义（均为Ｐ＜０．０１）。眼轴长度：戴镜前豚鼠双眼眼轴长度比较，差异无统计学意义（Ｐ＞０．０５）；戴镜后１周、２周、３周对照眼和实验眼的眼轴均较戴镜前明显延长，实验眼与对照眼的眼轴长度比较差异均有统计学意义（均为Ｐ＜０．０５）。免疫组织化学检测结果：双眼视网膜均可见部分视网膜神经节细胞胞浆及胞膜呈黄色或棕黄色着色，但实验眼免疫阳性细胞着色较深，阳性细胞数相对较多；早期豚鼠近视动物模型视网膜上ＭＦＮ１阳性表达主要见于视网膜神经节细胞，随着透镜诱导时间的延长，ＭＦＮ１在视锥视杆细胞中也出现表达，而对照眼无此现象。结论　采用－７．００Ｄ凹透镜能成功诱导光学离焦豚鼠近视动物模型，豚鼠视网膜上可见ＭＦＮ１表达，随着透镜诱导时间的延长，表达强度和部位逐渐发生变化，提示ＭＦＮ１可能在近视的发生发展过程中起着一定的作用。
Abstract:
Objective To establish the optical defocus induced myopia of guinea pig animal model and preliminarily explore the expression of mitochondrial fusion protein 1 ( MFNI) in the retina of in guinea pig lens induced myopia model. Methods Fifteen guinea pigs were obtained. One eye of the guinea pigs was randomly selected as the experimental eye and treated with - 7. 00 D lenses. The other eye was served as an internal control group. Ocular refraction and axial length were collected before treatment and I week.2 weeks ,3 weeks after treatment. After the completion of the refraction and axial length detection at I week.2 weeks, 3 weeks after treatment,5 guinea pigs were randomly selected, and MFNI expression in the retina of both eyes was tested by immunohistochenustry technique. Results The refraction data of the guinea pig eyes in the two groups indicated hyperopia before the treatment, and no significant difference was found between the two groups. The degree of hyperopia at 1 week,2 weeks,3 weeks after treatment decreased gradually in both eyes, no significant difference was found at I week after treatment between the experimental eyes and control eyes( P = 0. 380 ) . However, the refraction data were significantly different at 2 weeks and 3 weeks after treatment between the two groups ( all P < 0. 01 ) . There was no statistical difference in axial length before treatment between the two groups (P > 0. 05 ) , and the axial length at I week.2 weeks and 3 weeks after treatment in the two groups were all longer than that before treatment , the differences were significant between the two groups ( all P < 0. 05 ) . The immunohistochemistry results showed that MFNI positive cells could be observed in the retina of both eyes ,mainly in the cytoplasm and cell membrane of the ganglion cells , MFNI positive cells appeared brownish or yellow. In the experimental eyes , the immune positive cells were staining deep , and more positive cells could be observed . however , MFNI positive cells scattered expressed in the ganglion cell layer in the control eyes. Furthermore , MFNI positive expression could be seen mainly in ganglion cells at the first week of treatment .with the extension of lens induction time,many MFNI positive cells also appeared in the bipolar cell layer and the rod-cone cell layer, and this phenomenon could not be found in the control eyes. Conclusion The guinea pig myopia models are established successfully by wearing the - 7. 00 D concave lens. MFNI positive expression can be seen in the guinea pigs retina,with the extension of lens induction time .the intensity and position of the positive cells change gradually , which indicate that MFNI may play the certain role in the development of myopia.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号：８１３４１１０５）；四川省教育厅基金资助（编号：１３ＺＡ０２１５）
更新日期/Last Update: 2016-01-04