[1]杜娟,姜楠,邵毅,等.纤维蛋白黏合胶预防去势雄兔干眼症的实验研究[J].眼科新进展,2016,36(1):004-7.[doi:10.13389/j.cnki.rao.2016.0002]
　DU Juan,JIANG Nan,SHAO Yi,et al.Application of fibrin glue on xerophthalmia in castrated rabbit model[J].Recent Advances in Ophthalmology,2016,36(1):004-7.[doi:10.13389/j.cnki.rao.2016.0002]
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纤维蛋白黏合胶预防去势雄兔干眼症的实验研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年1期
页码:
004-7
栏目:
实验研究
出版日期:
2016-01-05

文章信息/Info

Title:
Application of fibrin glue on xerophthalmia in castrated rabbit model
作者:
杜娟; 姜楠; 邵毅; 胡佩宏; 张颖; 魏荣; 刘新华; 裴重刚
528300　广东省佛山市，佛山市顺德区第一人民医院，南方医科大学附属顺德第一人民医院眼科
Author(s):
DU Juan; JIANG Nan; SHAO Yi; HU Pei-Hong ; ZHANG Ying ;  WEI Rong ;  LIU Xin-Hua ;  PEI Chong-Gang
Department of Ophthalmology , the First People ’ s Hospital of Shunde , Foshan 528300 , Guangdong Province , China
关键词:
纤维蛋白黏合胶; 干眼症; 泪液蛋白
Keywords:
fibrin glue ;  xerophthalmia ;  tear protein
DOI:
10.13389/j.cnki.rao.2016.0002
文献标志码:
A
摘要:
目的　研究纤维蛋白黏合胶黏合泪道对雄兔去势所致干眼症的缓解作用。方法　选择新西兰白兔３６只（３６眼，均为右眼），剪除第三眼睑后，３ｇ·Ｌ－１氧氟沙星滴眼液滴眼１周，随后继续适应性喂养１周，取２４只雄兔制作去势雄兔干眼症模型，随机分为Ａ、Ｂ两组（各１２眼）：对照组（Ａ组）泪道注射生理盐水，实验组（Ｂ组）泪道内注射纤维蛋白黏合胶。剩余１２只（１２眼，Ｃ组）雄兔切开阴囊，不切除睾丸，眼部不予处理，作为假手术组。分别于注射前和注射后２周、４周及８周行ＳｃｈｉｒｍｅｒⅠ试验（ＳｃｈｉｒｍｅｒⅠ ｔｅｓｔ，ＳⅠＴ）、角膜荧光素（ｆｌｕｏｒｅｓｃｅ-ｉｎ，ＦＬ）染色、泪液蛋白测定，并于注射后２周、４周对Ｂ组泪道黏膜进行Ｋ１６免疫荧光染色及ＷｅｓｔｅｒｎＢｌｏｔ检查。结果　Ｃ组注射前及注射后各时间段各项检测差异均无统计学意义（均为Ｐ＞０．０５）。注射后２周、４周及８周，Ａ、Ｂ两组ＳⅠＴ、ＦＬ染色评分、泪液总蛋白含量、乳铁蛋白、溶菌酶及淀粉酶活性较注射前均有不同程度下降，其差异均有统计学意义（均为Ｐ＜０．０５），但注射后２周及４周Ｂ组ＳⅠＴ、ＦＬ染色评分下降程度较轻，与Ａ组下降程度相比差异均有统计学意义（均为Ｐ＜０．０５）。而Ｂ组泪液总蛋白量、乳铁蛋白、溶菌酶及淀粉酶活性则在注射后４周出现下降减缓，与Ａ组相比差异均有统计学意义（均为Ｐ＜０．０５）。免疫荧光染色和ＷｅｓｔｅｒｎＢｌｏｔ结果显示Ｂ组下睑泪道黏膜Ｋ１６阴性表达。结论　使用纤维蛋白黏合胶黏合泪道可较快而明显地改善干眼的症状和维持泪液蛋白成分，从而延缓去势雄兔干眼症的进展。
Abstract:
Objective To investigate the nutigative effects of conglutinated the lower lacrimal passage with fibrin glue on xerophthalmia in castrated rabbit model. Methods Thirty-six rabbits ( 24 right eyes) were chosen , and treated with 3 g . L -l ofloxacin eye drops for I week after cutting off the third eyelid,then all of the rabbits were fed 7 days to adapt the environment. The castrated dry eye model were established in 24 rabbits . and divided into two groups : the control group ( group A) injected the physiological saline in the lacrimal passage, and the experimental group ( group B) injected the fibrin glue ,12 cases in each group. The other 12 rabbits ( 12 eyes ,group C) were cut off the scrotum and saved the testes, setting as sham operation group. The Schirmer I test ( S IT) .comeal fluorescein ( FL) and tear protein determination were performed before injection and 2 weeks ,4 weeks.8 weeks after injection. The expression of K16 in lower lacrimal passage mucosa of the group B was examined by immunofluorescence staining and Western Blot at 2 weeks and 4 weeks after injection. ReSHlts There was no statistical difference in each detected index at different time before and after injection in group C ( all P > 0. 05 ) . S IT , FL , total proteins , lysozyme , lactoferrin and amylase activity at 2 weeks.4 weeks ,8 weeks after injection in group A and group B were all lower than those before injection ( all P < 0. 05 ) .and the decreased degree of S IT and FL at 2 weeks and 4 weeks after injection in group B was lower than those in group A ( all P < 0. 05 ) . The total proteins ,lysozyme , lactoferrin and amylase activity at 4 weeks after injection in group B showed the downward trend. there were statistical difference compared with group A ( all P < 0. 05 ) . The results of immunofluorescence and Westem Blot showed the expression of K16 in group B was negative in the lower lacrimal canaliculi. Conclusion Conglutinated the lower lacrimal passage with fibrin glue can improve the symptom of dry eye and maintain the component of tear protein . so delay the progress of the xerophthalmia in castrated rabbits.

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备注/Memo

备注/Memo:
国家自然科学基金资助（编号： ８１１６０１１８、８１４００３７２）；全国临床医药研究专项基金（编号：Ｌ２０１２０５２）；江西省远航工程（编号：２０１４０２２）；江西省学位与研究生教育教学改革研究项目（编号：２０１５）；江西省科技平台建设项目（编号：２０１３１１６）；江西省青年科学基金（编号：２０１５１ＢＡＢ２１５０１６）；江西省科技支撑计划项目（编号：２０１５１ＢＢＧ７０２２３）；江西省教育厅科技计划项目（编号：ＧＪＪ１４１７０）；江西省卫计委科技计划项目（编号：２０１５５１５４）；赣江学者计划（编号：２０１５）
更新日期/Last Update: 2016-01-04