[1]储三军,王敏,徐海峰.贝伐单抗对人脐静脉内皮细胞纤维化相关炎症因子表达的影响[J].眼科新进展,2015,35(11):1025-1028.[doi:10.13389/j.cnki.rao.2015.0280]
　CHU San-Jun,WANG Min,XU Hai-Feng.Effects of bevacizumab on expression of fibrosis-related inflammatory mediators in human umbilical vein endothelial cells[J].Recent Advances in Ophthalmology,2015,35(11):1025-1028.[doi:10.13389/j.cnki.rao.2015.0280]
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贝伐单抗对人脐静脉内皮细胞纤维化相关炎症因子表达的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年11期
页码:
1025-1028
栏目:
实验研究
出版日期:
2015-11-05

文章信息/Info

Title:
Effects of bevacizumab on expression of fibrosis-related inflammatory mediators in human umbilical vein endothelial cells
作者:
储三军; 王敏; 徐海峰
266071　山东省青岛市，青岛大学医学院，山东省眼科研究所
Author(s):
CHU San-Jun; WANG Min; XU Hai-Feng
Medical College of Qingdao University, Shandong Eye Institute, Qingda0 266071, Shandong Province . China
关键词:
贝伐单抗; 血管纤维化; 炎症因子; 人脐静脉内皮细胞
Keywords:
bevacizumab ; vessels fibrosis ; inflammatory cytokines ;  human umbilical vein endothelial cells
DOI:
10.13389/j.cnki.rao.2015.0280
文献标志码:
A
摘要:
目的　观察贝伐单抗对人脐静脉内皮细胞（ｈｕｍａｎｕｍｂｉｌｉｃａｌｖｅｉｎｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｓ，ＨＵＶＥＣ）纤维化相关炎症因子表达的影响，以探讨贝伐单抗治疗后新生血管纤维化的可能机制。方法　分别在正常氧含量及缺氧条件下培养ＨＵＶＥＣ，培养液中加入终浓度为０．２５ｇ·Ｌ－１贝伐单抗，根据处理时间的不同分为０ｈ组（对照组）、６ｈ组、１２ｈ组、２４ｈ组、４８ｈ组。分别用ＲＴ-ＰＣＲ及ＥＬＩＳＡ方法检测各组纤维化相关炎症因子白细胞介素（ｉｎｔｅｒｌｅｕｋｉｎ，ＩＬ）-１β、ＩＬ-６、ＩＬ-８以及肿瘤坏死因子-α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ-ａｌｐｈａ，ＴＮＦ-α）ｍＲＮＡ和蛋白的表达。结果　正常氧含量条件下，与对照组相比，ＩＬ-６、ＩＬ-８的ｍＲＮＡ和蛋白表达在各实验组均明显增加，而ＩＬ-１β、ＴＮＦ-αｍＲＮＡ和蛋白的表达仅在１２ｈ、２４ｈ、４８ｈ组明显增加，差异均有统计学意义（均为Ｐ＜０．０５）。缺氧条件下，与对照组相比各实验组ＩＬ-１β、ＩＬ-６、ＩＬ-８及ＴＮＦ-α的ｍＲＮＡ和蛋白表达在各时间点均明显增加，差异均有统计学意义（均为Ｐ＜０．０５）。结论　无论在正常氧含量还是缺氧条件下，贝伐单抗均可刺激纤维化相关炎症因子的表达增加，此部分炎症因子可能参与了抗新生血管内皮生长因子治疗后的新生血管纤维化过程。
Abstract:
Objective To investigate the effects of bevacizumab on the expressions of fibrosis-related inflammatory mediators in human umbilical vein endothelial cells ( HUVEC) , and further clarify the mechanism of fibrosis after the treatment of bevacizumab. Methods HUVEC were cultured under normal oxygen and hypoxia conditions , respectively. And bevacizumab were added into the culture medium. Cells were divided int0 5 groups according to incubation period :0 hour( control) ,6 hours . 12 hours. 24 hours and 48 hours. The mRNA and protein levels of interleukin ( 11)-1 [3 ,IL-6 , IL-8 , and tumor necrosis factor( TNF)~d were evaluated by SYBR green real-time PCR and ELISA, respectively. Results Under normoxic conditions, bevacizumab significantly increased the secretion of IL-6 and IL-8 at 6 hours ,12 hours.24 hours and 48 hours . and 11-1 [3 and TNF-e/ were only increased obviously at 12 hours.24 hours and 48 hours after the treatment of bevacizumab ( all P < 0. 05 ) . Bevacizumab significantly increased the secretion of 11-1 [3 , IL-6 .IL-8 , and TNF- eL under hypoxic conditions at 6 hours , 12 hours , 24 hours and 48 hours ( all P < 0. 05 ) . Conclusion Treatment of HUVEC with bevacizumab significantly increase the secretion of fibrosis-related inflammatory mediators. Those inflammatory cytokines may be involved in the fibrosis process after anti-vascular endothelial growth factor treatment.

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备注/Memo

备注/Memo:
山东省自然科学基金资助（编号：ＺＲ２０１４ＨＭ０２９）；青岛市科技计划项目（编号：１３-１-３-６９-ｎｓｈ）
更新日期/Last Update: 2015-11-03