[1]费远丽,高文,杨光. 不同移植方式对小鼠恶性黑色素移植瘤的生长及其血管生成的影响[J].眼科新进展,2015,35(4):326-329.[doi:10.13389/j.cnki.rao.2015.0087]
　FEI Yuan-Li,GAO Wen,YANG Guang. Effects of different transplantation methods on growth and angiogenesis of transplanted tumor of malignant melanoma in mice[J].Recent Advances in Ophthalmology,2015,35(4):326-329.[doi:10.13389/j.cnki.rao.2015.0087]
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 不同移植方式对小鼠恶性黑色素移植瘤的生长及其血管生成的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年4期
页码:
326-329
栏目:
实验研究
出版日期:
2015-04-05

文章信息/Info

Title:
 Effects of different transplantation methods on growth and angiogenesis of transplanted tumor of malignant melanoma in mice
作者:
 费远丽; 高文; 杨光
 300073　天津市，天津中医药大学
Author(s):
 FEI Yuan-Li ;  GAO Wen ; YANG Guang
 Tianjin University of Traditional Chinese Medicine, Tianjin 300073 , China
关键词:
 ; 恶性黑色素移植瘤; 血管生成; 血管内皮生长因子; 白细胞介素17; 白细胞介素23
Keywords:
 ; transplanted tumor of malignant melanoma;  angiogenesis;  vascular endothelial growth factor ;  interleukin 17 ;  interleukin 23
DOI:
10.13389/j.cnki.rao.2015.0087
文献标志码:
A
摘要:
 目的　研究不同移植方式（玻璃体移植、足垫皮下移植）对小鼠恶性黑色素移植瘤的生长及其血管生成的影响，并初步探讨炎症因子对此两种不同移植瘤的作用机理。方法　４０只Ｃ５７ＢＬ-６Ｊ小鼠编号后按照随机数字表法分成两组：玻璃体组２０只、足垫皮下组２０只。分别接种黑色素瘤Ｂ１６Ｆ１０细胞于左眼玻璃体和右足垫皮下，然后对肿瘤的发生率及瘤体的基本特征进行２７ｄ连续观察；分别于接种后９ｄ、１５ｄ、２１ｄ、２７ｄ取小鼠血清，ＥＬＩＳＡ检测血清中ＩＬ-１７、ＩＬ-２３、ＶＥＧＦ含量；接种后２８ｄ处死全部小鼠，摘取肿瘤组织称瘤质量，ＨＥ染色观察肿瘤病理特征及其肝、肺转移情况。结果　两组小鼠肿瘤发生率均为１００％。玻璃体组肝、肺转移发生率均显著大于足垫皮下组，差异均有统计学意义（χ２＝６．６７、６．６７，均为Ｐ＜０.０１）。玻璃体组小鼠肿瘤出现时间（８．７０±２．２７）ｄ早于足垫皮下组（８．９５±０．２２）ｄ，但两组间差异无统计学意义（ｔ＝０．４９，Ｐ＞０．０５）；２７ｄ时玻璃体组小鼠体质量（１８．８０±２．４２）ｇ较足垫皮下组小鼠（２０．２０±１．４４）ｇ减轻更明显，且两组差异有统计学意义（ｔ＝２．２２，Ｐ＜０．０５）；玻璃体组小鼠肿瘤组织质量（３．５１±０．５８）ｇ高于足垫皮下组（３．１８±０．６２）ｇ，但两组差异无统计学意义（ｔ＝１．７４，Ｐ＞０．０５）。接种后随着时间的推移两组小鼠血清中的ＩＬ-１７、ＩＬ-２３、ＶＥＧＦ含量均逐渐升高，９ｄ时玻璃体组血清ＩＬ-１７、ＩＬ-２３、ＶＥＧＦ含量均高于足垫皮下组，但差异均无统计学意义（均为Ｐ＞０．０５），１５ｄ、２１ｄ及２７ｄ时足垫皮下组血清ＩＬ-１７、ＩＬ-２３、ＶＥＧＦ含量均高于玻璃体组，差异均有显著统计学意义（均为Ｐ＜０．００１）。结论　把肿瘤相关炎症因子作为恶性黑色素瘤免疫治疗的新靶点，全程抗炎疗法将为眼内和皮肤黑色素瘤的治疗提供新的思路，且眼内黑色素瘤的早期治疗中积极运用抗炎药物尤为重要。
Abstract:
 Objective To investigate the effects of different transplantation methods ( intravitreal transplantation , subcutaneous transplantation) on the growth and angiogenesis of transplanted tumor of malignant melanoma in mice. and explore the mechanism of inflammatory factor in two different transplanted tumor. Methods Forty C57BL-6J mice were randomly divided into intravitreal group and subcutaneous group , 20 cases in each group. B16FIO melanoma cells were inoculated in the vitreous of left eye and the pad subcutaneous of right foot. then the basic characteristic of tumor and tumor incidence were observed continuously for 27 days. At 9 days, 15 days, 21 days and 27 days after inoculated. the specimens from the serum of mice were collected, the serum levels of interleukin 17 ( IL-17 ) , interleukin 23 ( IL-23) . vascular endothelial growth factor ( VEGF) were detected by ELISA. At 28 days after inoculated. all mice were sacrificed, then the tumor were extracted and weighed, HE staining was used to observe the pathological features and liver. lung metastasis. Result The tumor successful rate of two groups were all 100% . The liver and lung metastases in the vitreous group were sigruficantly greater than those in the footpad subcutaneous group , the differences were statistically sigruficant (X2 = 6. 67 , 6. 67 . both P < 0. 01) . Average tumor appearance time in vitreous cavity of mice ( 8. 70 +2. 27 ) days was earlier than that in the average footpad subcutaneous ( 8. 95 +0. 22) days , but no sigruficant difference was found between the two groups ( t = 0. 49 , P > 0. 05 ) . At 27 days after transplantation. the body mass of the vitreous group ( 18. 80 + 2. 42 ) g was lower than that of the footpad subcutaneous group (20. 20 + 1. 44) g, and the difference was statistically significant ( t = 2. 22 , P < 0. 05 ) . The average tumor mass of the vitreous group (3. 51 +0. 58) g was higher than that of the footpad subcutaneous group ( 3. 18 + 0. 62) g, but the difference was not statistically significant ( t = 1. 74 . P > 0. 05 ). The serum levels of IL-17 , IL-23 and VEGF were gradually increased with the time prolong , the serum levels of IL-17, IL-23 and VEGF at 9 days in the vitreous group were higher than those in the subcutaneous group , but the difference was not statistically significant ( all P > 0. 05 ) . At 15 days , 21 days and 27 days , the serum levels of IL-17 . IL-23 and VEGF in the subcutaneous group were higher than those in the vitreous group , and the differences were statistically significant ( all P < 0. 001 ) . Conclrision The tumor related inflammation factor IL-17 , IL-23 as a new target for immunotherapy of malignant melanoma. the whole course of anti-inflammatory therapy will provide a new idea for the treatment of ocular and cutaneous melanoma . and early actively treatment of intraocular melanoma with anti-inflammatory drug is particularly important.

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备注/Memo

备注/Memo:
 天津中医药大学第一附属医院“拓新工程”基金科研课题（编号：５４６）
更新日期/Last Update: 2015-03-31