[1]蔺静静,刘向玲,王圆月,等. 丰富环境及反转缝合治疗后突触素对关键期内视皮质神经元可塑性的影响[J].眼科新进展,2015,35(4):330-333.[doi:10.13389/j.cnki.rao.2015.0088]
　LIN Jing-Jing,LIU Xiang-Ling,WANG Yuan-Yue,et al. Effects of synaptophysin on plasticity of visual cortex neurons after rich environment and reverse suture treatment in critical period[J].Recent Advances in Ophthalmology,2015,35(4):330-333.[doi:10.13389/j.cnki.rao.2015.0088]
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 丰富环境及反转缝合治疗后突触素对关键期内视皮质神经元可塑性的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年4期
页码:
330-333
栏目:
实验研究
出版日期:
2015-04-05

文章信息/Info

Title:
 Effects of synaptophysin on plasticity of visual cortex neurons after rich environment and reverse suture treatment in critical period
作者:
 蔺静静; 刘向玲; 王圆月; 宋子宣; 李靳; 张锐
 453003　河南省新乡市，新乡医学院
Author(s):
 LIN Jing-Jing;  LIU Xiang-Ling;  WANG Yuan-Yue;  SONG Zi-Xuan;  LI Jin ; ZHANG Rui
 Xinxiang Medical University. Xinxiang 453003 , Henan Province , China
关键词:
 ; 单眼形觉剥夺; 弱视; 突触素; 视皮质; 反转缝合治疗; 丰富环境
Keywords:
 ; moncular form deprived;  amblyopia;  synaptophysin;  visual cortex;  reverse suture ;  enriched environment
DOI:
10.13389/j.cnki.rao.2015.0088
文献标志码:
A
摘要:
 目的　探讨关键期内单眼形觉剥夺性弱视大鼠模型经反转缝合及丰富环境治疗前后视皮质突触素（ｓｙｎａｐｔｏｐｈｙｓｉｎ，ＳＹＰ）的表达规律，及ＳＹＰ对视皮质神经元可塑性的影响。方法　３６只１４ｄ龄ＳＤ大鼠随机分为６组，每组６只，其中ＮＣＩ组、ＮＣＩＩ组为正常对照组；ＭＤＩ组、ＭＤＩＩ组、反转缝合（ｒｅｖｅｒｓｅｓｕｔｕｒｅ，ＲＳ）组和ＲＳ＋丰富环境（ｅｎｒｉｃｈｅｄｅｎｖｉｒｏｎｍｅｎｔ，ＥＥ）组分别行右眼眼睑缝合，ＲＳ组、ＲＳ＋ＥＥ组于２８ｄ龄打开剥夺眼，同时缝合对侧眼睑行遮盖治疗，ＲＳ组放于标准环境中饲养、ＲＳ＋ＥＥ组放于ＥＥ中饲养；ＮＣＩ组、ＭＤＩ组于２８ｄ龄，ＮＣＩＩ组、ＭＤＩＩ组、ＲＳ组、ＲＳ＋ＥＥ组于４２ｄ龄行图形视觉诱发电位检测，同时采用ＨＥ染色和免疫组织化学法检测分析各组大鼠视皮质ＳＹＰ蛋白表达水平的变化。结果　图形视觉诱发电位示形觉剥夺眼Ｐ１００波的波形稳定性差、潜伏期延长、振幅降低。ＨＥ染色示各组大鼠视皮质神经元均未见明显异常。免疫组织化学法检测可见ＲＳ组视皮质ＳＹＰ阳性神经元密度较ＭＤＩＩ组高，ＲＳ＋ＥＥ组视皮质ＳＹＰ阳性神经元密度较ＭＤＩＩ组、ＲＳ组高，ＭＤＩ组大鼠视皮质ＳＹＰ阳性神经元密度较ＮＣＩ组低，ＭＤＩＩ组、ＲＳ组大鼠视皮质ＳＹＰ阳性神经元密度均较ＮＣＩＩ组及ＲＳ＋ＥＥ组低，定量分析显示以上各组ＳＹＰ免疫标记的平均光密度值间差异均有统计学意义（均为Ｐ＜０．０５），但ＮＣＩＩ组和ＲＳ＋ＥＥ组两组间平均光密度值差异无统计学意义（Ｐ＞０．０５）。结论　ＳＹＰ影响视皮质神经元可塑性，且参与了视觉发育视皮质可塑性的变化过程，是弱视发病的重要分子机制之一；关键期内ＲＳ＋ＥＥ治疗对弱视视皮质神经元功能状态的恢复有促进作用。
Abstract:
 Objective To investigate the expression of synaptophysin ( SYP) and its effects on plasticity of neurous in the visual cortex of monocularly deprived rats before and after reverse suture combined with enriched environment treatment in the critical period. Methods Thirty-six 14-day-old rats were randomly divided into six groups , 6 cases in each group , in which normal control group I ( NCI group) and normal control group II ( NCII group) were the normal control group, the right eyelid of rats in monocular deprivation group I ( MDI group) , monocular deprivation group II ( MDII group) , reverse suture group ( RS group) and RS + enriched environment group ( RS + EE group) were sutured, the eyelid of 28-day-old rats in RS group and RS + EE group were opened, then the other side eyelid were sutured. The rats in RS group were bred in the normal environment , and RS + EE group bred in EE. The pattern visual evoked potential of 28-day-old rats in NCI group and MDI group , 42-day-old rats in NCII group, MDII group, RS group and RS + EE group were measured, and the expression of SYP protein in the visual cortex of the different groups were detected by immunohistochemistry staining and HE staining. Results The pattern visual evoked potential showed that the stability of PlOO wave in the deprived eye was poor, the latency was increased. and the amplitude was decreased. The HE stairung showed that the visual cortex neurons of rats in each group unchanged. The immunohistochenucal staining showed that: The density of positive neurons of SYP in the visual cortex of RS group was sigruficantly higher than those in MDII group , RS + EE group was higher than MDII group and RS group , MDII group was lower than NCI group , MDII group and RS group were lower than MDII group and RS + EE group ; The quantitative analysis showed that. except for NCII group and RS + EE group, there were statistical differences in density of positive neurons between each other two groups ( all P < 0. 05 ) . Conclusion SYP affect the plasticity of neurons and participate in the plasticity of visual development, which were one of the key moleculars in the mechanism of amblyopia pathogenesis. The cover treatment and enriched environment may partially reverse the neural activity of deprived amblyopia in the visual cortex during the critical period.

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备注/Memo

备注/Memo:
 河南省教育厅２０１１年医学科技攻关项目（编号：２０１１Ａ３２００１２）
更新日期/Last Update: 2015-03-31