[1]康丽华,管怀进,张国伟,等. 热休克蛋白A12B在大鼠角膜、晶状体和视网膜组织中的表达[J].眼科新进展,2015,35(3):223-226.[doi:10.13389/j.cnki.rao.2015.0060]
　KANG Li-Hua,GUAN Huai-Jin,ZHANG Guo-Wei,et al. Expression of heat shock protein A12B in cornea,lens and retina tissue of rats[J].Recent Advances in Ophthalmology,2015,35(3):223-226.[doi:10.13389/j.cnki.rao.2015.0060]
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 热休克蛋白A12B在大鼠角膜、晶状体和视网膜组织中的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年3期
页码:
223-226
栏目:
实验研究
出版日期:
2015-03-05

文章信息/Info

Title:
 Expression of heat shock protein A12B in cornea,lens and retina tissue of rats
作者:
 康丽华; 管怀进; 张国伟; 秦柏; 陈佳; 张俊芳; 杨铃
 226001　江苏省南通市，南通大学附属医院眼科
Author(s):
 KANG Li-Hua;  GUAN Huai-Jin ;  ZHANG Guo-Wei;  QIN Bai; CHEN Jia ;  ZHANG Jun-Fang;  YANG Ling
 Department of Ophthalmology , Affiliated Hospital of Nantong University , Nantong 226001 , Jiangsu Province . China
关键词:
 ; 角膜; 晶状体; 视网膜; 热休克蛋白A12B; 大鼠
Keywords:
 ; cornea ; lens ;  retina ;  heat shock protein A12B ;  rats
DOI:
10.13389/j.cnki.rao.2015.0060
文献标志码:
A
摘要:
 目的　观察大鼠角膜、晶状体、视网膜中热休克蛋白Ａ１２Ｂ（ｈｅａｔｓｈｏｃｋｐｒｏｔｅｉｎＡ１２Ｂ，ＨＳＰＡ１２Ｂ）的表达。方法　取ＳＰＦ级ＳＤ大鼠９只，用水合氯醛处死后立即摘取眼球，采用Ｗｅｓｔｅｒｎｂｌｏｔ和ＲＴ-ＰＣＲ分别检测大鼠角膜、晶状体和视网膜组织中ＨＳＰＡ１２Ｂ蛋白和ｍＲＮＡ的表达情况；免疫荧光化学法观察ＨＳＰＡ１２Ｂ在角膜、晶状体和视网膜组织中的表达及定位。结果　Ｗｅｓｔｅｒｎｂｌｏｔ和ＲＴ-ＰＣＲ检测结果显示：ＨＳＰＡ１２Ｂ在大鼠角膜、晶状体、视网膜均有少量表达，其中角膜组织（０．２８０±０.０３４、０．３７４±０.０３１）中表达强度相对最低，晶状体（０．３３１±０．０３６、０．４５３±０．０４０）次之，视网膜组织（０．４５３±０．０２９、０．６８０±０．０４５）中表达强度最高。免疫荧光化学法结果表明，在角膜组织中ＨＳＰＡ１２Ｂ主要表达于上皮层，其中基底细胞染色最强，而角膜基质细胞上仅有弱表达；在晶状体组织中ＨＳＰＡ１２Ｂ主要表达于上皮细胞和皮质；在视网膜组织中ＨＳＰＡ１２Ｂ主要表达于视网膜神经纤维层、神经节细胞层和内核层，并与ＮｅｕＮ标记的神经元及ＧＳ标记的Ｍüｌｌｅｒ细胞存在共定位。结论　ＨＳＰＡ１２Ｂ在大鼠角膜、晶状体、视网膜组织中均有表达，且呈特异性表达。
Abstract:
 Objective To explore the expression of heat shock protein A12B ( HSPA12B) in cornea.lens and retina tissue of rats. Methods The cornea.lens and retina tissue from 9 SPF SD rats were isolated. Western blot and RT-PCR assay were used to detect the protein and mRNA expressions of HSPA12B in cornea.lens and retina tissue. Immunofluorescence stairung was used to observe the location of HSPA12B in cornea.lens and retina tissue. Results Western blot and RT-PCR analysis revealed that HSPA12B were expressed in cornea,lens and retina tissue of rats. The expression of HSPA12B was the weakest in cornea (0. 280 + 0. 034 ,0. 374 + 0. 031 ) , moderated in lens (0. 331 + 0. 036 , 0. 453 + 0. 040 ) , but it was strongest in retina ( 0. 453 + 0. 029 . 0. 680 +0. 045 ) . Immunofluorescence stairung showed that HSPA12B mainly located at corneal epithelium with the strongest staining in the basal cells. but in contrast weak staining was seen in corneal stroma cells. HSPA12B was strongly expressed in epithelial cells and shallow cortex layer of lens. In retina , HSPA12B was extensively and richly expressed in retinal nerve fiber layer, ganglion cell layer and inner nuclear layer. The colocalization of HSPA12B with the neuron marked by NeuN and the miiller cell marked by GS in retinal tissue. Conclrision HSPA12B is proved to express specificity in cornea.lens and retina tissue.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：８１２７０９８７、８１４７０６１６）
更新日期/Last Update: 2015-03-04