[1]化志娟,许静,周圆,等. 形觉剥夺性弱视幼猫视皮质17区生长相关蛋白-43的表达[J].眼科新进展,2015,35(3):227-230.[doi:10.13389/j.cnki.rao.2015.0061]
　HUA Zhi-Juan,XU Jing,ZHOU Yuan,et al. Expression of GAP-43 in 17 area of visual cortex in form-deprived amblyopic cats[J].Recent Advances in Ophthalmology,2015,35(3):227-230.[doi:10.13389/j.cnki.rao.2015.0061]
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 形觉剥夺性弱视幼猫视皮质17区生长相关蛋白-43的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年3期
页码:
227-230
栏目:
实验研究
出版日期:
2015-03-05

文章信息/Info

Title:
 Expression of GAP-43 in 17 area of visual cortex in form-deprived amblyopic cats
作者:
 化志娟; 许静; 周圆; 胡敏
 650021　云南省昆明市，云南省第二人民医院眼科
Author(s):
 HUA Zhi-Juan;  XU Jing;  ZHOU Yuan;  HU Min
 Department of Ophthatmology,the Second People ’s Hospital of Yunnan Province , Kunming 650021 , Yunnan Province , China
关键词:
 ; 形觉剥夺; 弱视; 生长相关蛋白-43; 左旋多巴
Keywords:
 ; form-deprived ;  amblyopia ;  growth associated protein-43 ; levodopa
DOI:
10.13389/j.cnki.rao.2015.0061
文献标志码:
A
摘要:
 目的　观察形觉剥夺性弱视动物模型应用左旋多巴（ｌｅｖｏｄｏｐａ，ＬＤ）治疗后视皮质１７区生长相关蛋白-４３（ｇｒｏｗｔｈａｓｓｏ-ｃｉａｔｅｄｐｒｏｔｅｉｎ-４３，ＧＡＰ-４３）的表达，探讨ＬＤ对形觉剥夺性弱视视觉功能恢复作用的可能机制。方法　５０只４周龄幼猫，随机分为正常组、剥夺组、对照组、低剂量ＬＤ组及高剂量ＬＤ组共５组，每组１０只。通过单眼眼睑缝合制备形觉剥夺的弱视模型，观察不同干预条件下各组图形视觉诱发电位（ｐａｔｔｅｒｎｖｉｓｕａｌｅｖｏｋｅｄｐｏｔｅｎｔｉａｌ，ＰＶＥＰ）的Ｐ１００波的峰时及振幅的变化。应用免疫组织化学法测定各组视皮层１７区ＧＡＰ-４３的差异。结果　８周龄时，剥夺组、对照组、低剂量ＬＤ组、高剂量ＬＤ组剥夺眼Ｐ１００波振幅比对侧眼及同周龄正常组幅值降低，潜时延长（均为Ｐ＜０．０５）；１２周龄时，低剂量ＬＤ组剥夺眼Ｐ１００波振幅增高，与对侧眼及同周龄正常组相比差异均无统计学意义（均为Ｐ＞０．０５），而Ｐ１００波潜时则未达到正常（Ｐ＜０．０５）；高剂量ＬＤ组剥夺眼Ｐ１００波振幅增高、潜时缩短，与对侧眼及同周龄正常组相比差异均无统计学意义（均为Ｐ＞０．０５）。８周龄时剥夺组视皮质１７区Ⅱ／Ⅲ、Ⅳ、Ⅴ／Ⅵ层ＧＡＰ-４３免疫阳性细胞密度均低于正常组，差异均有统计学意义（均为Ｐ＜０．０５）；１２周龄时高剂量ＬＤ组各层ＧＡＰ-４３免疫阳性细胞密度与正常组基本相等，差异无统计学意义（Ｐ＞０.０５）；对照组、低剂量ＬＤ组和正常组相比差异均有统计学意义（均为Ｐ＜０．０５）。结论　ＧＡＰ-４３在形觉剥夺性弱视形成过程中起一定的作用，ＬＤ对形觉剥夺性弱视视功能改善的机制是通过ＧＡＰ-４３表达的改变而实现的。
Abstract:
 Objective To investigate expression of growth associated protein-43 ( GAP-43 ) , a growth associated protein .in 17 area of visual cortex in monocular formdeprived amblyopia model after treated with levodopa ( LD ) . and discuss the possible mechanism of monocular form-deprived amblyopia and therapeutic effect of LD. Methods Fifty cats with 4-week-old were randomly divided into five groups :normal group , monocular form-deprived group , control group , low-dose group , high-dose group , ten cats in each group. The arumal models of monocular form deprived amblyopia were established. Pattern-visual evoked potential ( P-VEP) was recorded to check the visual acuity of the cats. The cell number density of GAP-43 expression in visual cortex area 17 was detected and calculated by immunohistochemistry. Results At 8-week-old, the latency and amplitude of PlOO of PVEP was significantly delayed and declined in monocular deprived eyes of monocular form-deprived group control group , low dose and high dose group , there was statistical difference compared with fellow eyes and normal eyes (P < 0. 05 ). At-12-week-old. the amplitude of low dose group in monocular deprived eyes had no sigruficant difference compared with fellow eyes and normal eyes ( all P > 0. 05 ) ; But the latency did not recover to normal (P < 0. 05 ) . In high dose group , the amplitude and latency recovered to normal. there was no statistical difference compared with fellow eyes and normal eyes ( all P > 0. 05 ) . The density of GAP-43 immunopositive cells of the monocular form-deprived group in visual cortex area 17 was significantly lower than that of the normal group [ ( 180. 05 +4. 96) mm -- vs ( 188. 17 + 7. 82) mm -z ,P < 0. 05 ) . However, there was no sigruficant difference between the highdose group and normal group [ ( 198. 50 + 2. 92 ) mm -z vs ( 200. 98 + 9. 64 ) mm -2 ,p > 0. 05 ) . Compared with the normal group , there was significant difference between the control group and the low-dose group ( P < 0. 05 ) . Conclusion GAP-43 play an important role in formation of amblyopia , the improved visual function of amblyopia may be brought into effect through GAP-43 expression.

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备注/Memo

备注/Memo:
 云南省自然科学基金资助（编号：２０１０ＣＤ１２２）
更新日期/Last Update: 2015-03-04