[1]张翀,陈蕾,李婷婷.血管生成素-1对小鼠视网膜新生血管和血管内皮生长因子的抑制作用[J].眼科新进展,2015,35(1):031-34.[doi:10.13389/j.cnki.rao.2015.0009]
　ZHANG Chong,CHEN Lei,LI Ting-Ting.Inhibitory effects of angiopoietin-1 on retinal neovas-cularization and VEGF of mice[J].Recent Advances in Ophthalmology,2015,35(1):031-34.[doi:10.13389/j.cnki.rao.2015.0009]
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血管生成素-1对小鼠视网膜新生血管和血管内皮生长因子的抑制作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年1期
页码:
031-34
栏目:
实验研究
出版日期:
2015-01-05

文章信息/Info

Title:
Inhibitory effects of angiopoietin-1 on retinal neovas-cularization and VEGF of mice
作者:
张翀; 陈蕾; 李婷婷
１１００３１　辽宁省沈阳市，沈阳市第四人民医院眼科
Author(s):
ZHANG Chong ;  CHEN Lei ;  LI Ting-Ting
Department of Ophthalmology, the Fourth People’s Hospital of Shenyang , Shenyang 110031 , Liaoning Province. China
关键词:
血管生成素-1; 视网膜新生血管; 血管内皮生长因子
Keywords:
angiopoietin-l;  retinal neovascularization;  vascular endothelial growth factor
DOI:
10.13389/j.cnki.rao.2015.0009
文献标志码:
A
摘要:
目的　探讨血管生成素１（ａｎｇｉｏｐｏｉｅｔｉｎ１，Ａｎｇ１）体内抑制视网膜新生血管形成的作用及对血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＶＥＧＦ）表达的影响。方法　高浓度氧诱导Ｃ５７ＢＬ／６Ｊ小鼠建立视网膜缺血性病变动物模型。将３２只小鼠随机分为４组：正常组、高氧对照组、大剂量治疗组（高氧＋１００ｍｇ?Ｌ－１Ａｎｇ１）、小剂量治疗组（高氧＋５０ｍｇ?Ｌ－１Ａｎｇ１）。前两组小鼠玻璃体内注射１μＬＢＳＳ，后两组小鼠玻璃体内分别注射１μＬ不同浓度的Ａｎｇ１。视网膜切片ＨＥ染色，计数并比较各组小鼠视网膜新生血管内皮细胞核数。免疫组织化学染色法检测各组小鼠视网膜切片ＶＥＧＦ的表达情况。结果　吸入高氧小鼠视网膜均可见突出内界膜的新生血管内皮细胞，发生率为１００％。高氧对照组可见突出细胞数为（２４．６９±２．６１）个，大剂量治疗组为（６．５６±１．３７）个，小剂量治疗组为（１０．８３±１．８１）个。大小剂量治疗组与高氧对照组相比新生血管细胞数明显减少（Ｐ＜０．０１）。在Ａｎｇ１治疗的两组中，随着剂量的加大，抑制视网膜新生血管形成的作用逐渐加强。高氧对照组（４．５０±０．２２）、大剂量治疗组（３．２９±０．１８）、小剂量治疗组（３．８１±０．１６）视网膜各层中ＶＥＧＦ表达均较正常组（２．２３±０．２０）明显增强，差异均有统计学意义（均为Ｐ＜０．０５）；与高氧对照组相比，大、小剂量治疗组ＶＥＧＦ表达水平明显降低（Ｐ＜０．０５）。结论　Ａｎｇ１能有效抑制高氧诱导的小鼠视网膜新生血管形成，且能减少ＶＥＧＦ的合成和分泌，其抗血管新生作用可能与抑制ＶＥＧＦ表达有关。
Abstract:
Objective To investigate the inhibitory effects of angiopoietin-l (Ang-l) on retinal neovascularization zn vzvo and the influence of Ang-I on vascular endothelial growth factor ( VEGF) expression. Methods C57BL/6J mice were put into the environment of high oxygen to establish models of ischemic retinopathy. Thirty-two mice were randomly divided into four groups : normal group , control group , high concentration group ( high oxygen + 100 mg ’ L-l Ang-1) , low concentration group ( high oxygen +50 mg . L -l Ang-I) . The first two groups received the intravitreal injection of I VL PBS , and the other two groups received intravitreal injection of I VL Ang-I with different concentration. HE stairung of retina was used to count and compare the endotheliocyte nuclei of new vessels extending from retina to vitreous in the tissue slice. Immunohistochemistry was used to evaluate the expression of VEGF. Results The incidence of retinal neovascular endotheliocyte in the eyes of hyperoxic groups was 100% . The neovascular endotheliocyte in the control group , high and low concentration groups were 24. 69 +2. 61 .6. 56 + 1. 37 and 10. 83 + 1. 81 , respectively. The number of endotheliocyte nuclei of new vessels in high and low concentration groups were less than those in the control group (P < 0. 01) . With the increase of the concentration of Ang-I , the inhibitory effect was enhanced. Compared with the normal group ( 2. 23 +0. 20) .the expressions of VEGF in the retina were obviously enhanced in the control group (4. 50 +0,22) , high concentration group ( 3. 29 + 0. 18) and low concentration group ( 3. 81 + 0. 16 ) . there were statistical differences ( all P < 0. 05 ) . Compared with the control group,the expression of VEGF were decreased in the high and low concentration groups ( P < 0. 05 ) . Conclusion Ang-1 can inhibit the retinal neovascularization induced by hyperxia. and decrease the expression of VEGF.which may be related with its inhibitory effect on VEGF expression.

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更新日期/Last Update: 2015-01-04