[1]缪进,李瑶,刘春,等.TGFα/EGFR通路相关蛋白在B6-Co小鼠眼睑中的表达[J].眼科新进展,2015,35(1):028-31.[doi:10.13389/j.cnki.rao.2015.0008]
　MIAO Jin,LI Yao,LIU Chun,et al.Expression of TGFα/EGFR signal pathway-associated protein in B6-Co mice embryonic eyelids[J].Recent Advances in Ophthalmology,2015,35(1):028-31.[doi:10.13389/j.cnki.rao.2015.0008]
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TGFα/EGFR通路相关蛋白在B6-Co小鼠眼睑中的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年1期
页码:
028-31
栏目:
实验研究
出版日期:
2015-01-05

文章信息/Info

Title:
Expression of TGFα/EGFR signal pathway-associated protein in B6-Co mice embryonic eyelids
作者:
缪进; 李瑶; 刘春; 宋鸿雁; 邵义祥
２２６００１　江苏省南通市，南通大学实验动物中心，南通大学比较医学研究所
Author(s):
MIAO Jin ;  LI Yao ;  LIU Chun ;  SONG Hong-Yan ;  SHAO Yi-Xiang
Laboratory Animal Center. Comparative Medicine Institute of Nantong University , Nantong 226001 , Jiangsu Province . China
关键词:
B6-Co小鼠; 眼睑; 转化生长因子α; 表皮生长因子受体; 细胞外调节蛋白激酶
Keywords:
B6-Co mice ;  eyelid;  transfornung growth factor a ;  epidermal growth factor receptor;  extracellular regulated protein kinases
DOI:
10.13389/j.cnki.rao.2015.0008
文献标志码:
A
摘要:
目的　研究转化生长因子α（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒα，ＴＧＦα）／表皮生长因子受体（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒｒｅｃｅｐｔｏｒ，ＥＧＦＲ）通路相关蛋白在Ｂ６Ｃｏ胎鼠眼睑中的表达情况，探讨Ｂ６Ｃｏ小鼠眼睑发育异常的原因。方法　取胚胎１６．５ｄ、１７．５ｄ、１８．５ｄ的Ｂ６和Ｂ６Ｃｏ胎鼠头部，做冰冻切片，通过免疫荧光法检测各时间点ＴＧＦα在眼睑部位的原位表达情况；提取蛋白，采用Ｗｅｓｔｅｒｎｂｌｏｔ法检测ＴＧＦα表达及ＥＧＦＲ、细胞外调节蛋白激酶（ｅｘｔｒａｃｅｌｌｕｌａｒｒｅｇｕｌａｔｅｄｐｒｏｔｅｉｎｋｉｎａｓｅ，ＥＲＫ）磷酸化蛋白表达情况。结果　免疫荧光检测结果示，ＴＧＦα主要表达于眼睑间质区，３个时间点Ｂ６Ｃｏ胎鼠眼睑中ＴＧＦα表达的荧光强度均高于同一时间点在Ｂ６胎鼠眼睑中的表达。Ｗｅｓｔｅｒｎｂｌｏｔ检测结果示，３个时间点Ｂ６Ｃｏ胎鼠眼睑中ＴＧＦα、ｐＥＧＦＲ的表达与Ｂ６胎鼠相比，差异均无统计学意义（均为Ｐ＞０．０５）。ｐＥＲＫ在胚胎１６．５ｄ和１７．５ｄ时，Ｂ６Ｃｏ胎鼠眼睑中的表达量分别为３．７９±０．３２和３．７７±０．１６，均显著高于在相应时间点Ｂ６胎鼠眼睑中的表达量１４８±０．１１和１．４５±０．０７，差异均有统计学意义（均为Ｐ＜０．０５）。结论　ＴＧＦα／ＥＧＦＲ通路的下游因子ＥＲＫ可能是导致Ｂ６Ｃｏ小鼠眼睑异常发育的重要因子。
Abstract:
Objective To investigate the expression of transfornung growth factor a ( TGFa)/ epidermal growth factor receptor ( EGFR) signal pathway-associated protein in embryonic eyelids, and explore the abnormal eyelid developmental mechanism in B6-Co mice. Methods E16. 5 days , E17. 5 days,E18. 5 days B6 and B6-Co embryonic heads were collected , and frozen sections were made by cryotomy. Immunofluo-rescence staining for TGFa was applied in the fetuses eyelids, and Western blot was used to analyze the expression of TGFa, p-EGFR and p-extracellular regulated protein kinases ( p-ERK) both in B6 and B6-Co mice. Results TGFa mainly expressed in the mesenchyme and fluorescence intensity in all B6-Co mice were higher than those in B6 mice at E16. 5 days,E17. 5 days ,E18. 5 days. There was no statistical difference in TGFa and p-EGFR expression between B6-Co and B6 mice detected by Western blot ( all P > 0. 05 ) . The p-ERK expression at E16. 5 days , E17. 5 days in B6-Co mice embryonic eyelids were 3. 79 +0. 32 and 3. 77 +0. 16 , respectively , which in B6 nuce were l. 48 +0. 11 and l. 12 + 0. 09 , respectively , there were statistical differences ( all P < 0. 05 ) . Conclusion ERK in TGFa/EGFR signal pathway may be one of the most significant downstream factors leading abnormal eyelid development in B6-Co mice.

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[1]吴进 于红奎 曹娟 张莉 夏焙 张越骊 钟晖.儿童眼睑毛母质瘤21例病例分析[J].眼科新进展,2012,32(5):000.
[2]宋鸿雁,仇保丰,刘春,等.应用16SrDNA克隆文库法分析B6-Co小鼠角膜炎细菌组成[J].眼科新进展,2014,34(12):1124.[doi:10.13389/j.cnki.rao.2014.0312]
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备注/Memo

备注/Memo:
江苏省高校自然科学基金资助（编号：１３ＫＪＢ１８００１９）
更新日期/Last Update: 2015-01-04