[1]沈天怡,刘俊伶,蔡雯婷,等.针刺治疗绝经后期干眼患者的疗效及其泪液蛋白质组学研究[J].眼科新进展,2018,38(10):959-963.[doi:10.13389/j.cnki.rao.2018.0227]
　SHEN Tian-Yi,LIU Jun-Ling,CAI Wen-Ting,et al.Proteomic analysis of tears following acupuncture treatment for menopausal dry eye based on two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry[J].Recent Advances in Ophthalmology,2018,38(10):959-963.[doi:10.13389/j.cnki.rao.2018.0227]
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针刺治疗绝经后期干眼患者的疗效及其泪液蛋白质组学研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
38卷
期数:
2018年10期
页码:
959-963
栏目:
应用研究
出版日期:
2018-10-05

文章信息/Info

Title:
Proteomic analysis of tears following acupuncture treatment for menopausal dry eye based on two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry
作者:
沈天怡; 刘俊伶; 蔡雯婷; 危清泉; 余咚卉; 金惠子; 梁秀玮; 于靖
200072　上海市，同济大学附属第十人民医院眼科
Author(s):
SHEN Tian-Yi; LIU Jun-Ling; CAI Wen-Ting; WEI Qing-Quan; YU Dong-Hui; JIN Hui-Zi; LIANG Xiu-Wei; YU Jing
Department of Ophthalmology，the Tenth People’s Hospital of Tongji University，Shanghai 200072，China
关键词:
针刺; 绝经后期; 干眼; 蛋白质组学
Keywords:
acupuncture; postmenopause; dry eye; proteomics
分类号:
R777.1
DOI:
10.13389/j.cnki.rao.2018.0227
文献标志码:
A
摘要:
目的　研究针刺疗法治疗绝经后期干眼患者的有效性及其分子机制。方法　选取28例（56眼）绝经后期干眼妇女，随机分成单纯人工泪液（artificial tears，AT）治疗组（AT组）14例28眼及针刺联合人工泪液 （acupuncture plus artificial tears，AC+AT） 治疗组（AC+AT组）14例28眼，每组均治疗2个月，并对治疗前后两组患者眼表疾病指数 （ocular surface disease index，OSDI）评分、症状评分、体征评分以及泪膜破裂时间（break-up time，BUT） 进行分析。同时采集治疗前后各组泪液样本，采用二维纳米液相色谱耦合串联质谱法 （two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry，2D Nano-LC-MS/MS）对AC+AT组治疗前后泪液进行蛋白质组学分析，采用蛋白质印记法比较两组泪液蛋白差异。结果　两组治疗后OSDI评分、症状评分、体征评分和BUT，AT组分别为39.08±18.68、7.43±2.08、11.46±2.29、（4.75±1.74）s，AC+AT组分别为24.55±17.43、4.68±2.54、11.21±2.96、（4.57±2.71）s；与治疗前相比均明显改善，差异均有统计学意义（均为P=0.000）。与AT组相比，针刺治疗后AC+AT组的症状评分明显改善（P=0.000）。2D Nano-LC-MS/MS法分析了泪液中2411种蛋白，其中142种表达下调，169种表达上调。与AT组相比，针刺治疗后AC+AT组患者泪液中细胞质蛋白表达下调（P=0.000），分泌蛋白表达上调（P=0.000）。在蛋白功能方面，与AT组相比，AC+AT组细胞组分蛋白表达下调 （P=0.000），免疫蛋白表达上调 （P=0.040）。结论　AC+AT治疗增加了泪液蛋白质合成及分泌，改善临床绝经后期干眼患者症状。针刺治疗可能成为治疗该类干眼患者的有效辅助疗法。
Abstract:
Objective　To investigate whether acupuncture is an effective treatment for dry eye among postmenopausal women and identify the molecular mechanisms of acupuncture.Methods　Twenty-eight postmenopausal women with dry eye （fifty-eight eyes） were randomly divided into two groups：artificial tears （AT） group including fourteen patients with twenty-eight eyes and acupuncture plus artificial tears （AC+AT） group including fourteen patients with twenty-eight eyes.Each patient received the topical therapy for 2 months.Pretreatment and posttreatment data of ocular surface disease index （OSDI） scores，symptom assessment scores，sign assessment scores，tear break-up time （TBUT） were analyzed.Meanwhile，tear samples of both groups were collected.Tear samples from AC+AT group were subjected to two-dimensional nano-liquid chromatography coupled with tandem mass spectrometry （2D Nano-LC-MS/MS） before and after treatment.Western blot analysis was performed on tear samples from both groups.Results　After treatment，scores of OSDI，symptom assessment，sign assessment and TBUT were significant improved in both groups than before therapy （P=0.000），which represented 39.08±18.68，7.43±2.08，11.46±2.29 and （4.75±1.74）s in AT group，while represented 24.55±17.43，4.68±2.54，11.21±2.96，（4.57±2.71）s in AC+AT group，respectively.Compared with AT group，symptom assessment scores were improved than those in AC+AT group significantly （P=0.000）.2D Nano-LC-MS/MS identified 2411 proteins，among which 142 kinds of proteins were down-regulated and 169 were upregulated.When compared with AT group，the expression of cytoplasmic proteins was down-regulated，while the expression of secreted proteins was up-regulated in AC+AT group after treatment （both P=0.000）.In the aspect of function，the expression of proteins involved in components was down-regulated，while the expression of immunity protein was upregulated （P=0.040）.Conclusion　AC+AT treatment increases the synthesis and secretion of proteins，and improves clinical symptoms in postmenopausal dry eye，which indicates that acupuncture may be an effective complimentary therapy for treating postmenopausal dry eye.

参考文献/References:

［1］　CALONGE M.The treatment of dry eye［J］.Surv Ophthalmol，2001，45（Suppl 2）：S227-S239.
［2］　TRUONG S，COLE N，STAPLETON F，GOLEBIOWSKI B.Sex hormones and the dry eye［J］.Clin Exp Optom，2014，97（4）：324-336.
［3］　FENG Y，FENG G，PENG S，LI H.The effects of hormone replacement therapy on dry eye syndromes evaluated by Schirmer text depend on patient age［J］.Cont Lens Anterior Eye，2016，39（2）：124-127.
［4］　MANSON J E，HSIA J，JOHNSON K C，ROSSOUW J E，ASSAF A R，LASSER N L，et al.Estrogen plus progestin and the risk of coronary heart disease［J］.N Engl J Med，2003，349（6）：523-534.
［5］　BERAL V，BULL D，REEVES G.Endometrial cancer and hormone-replacement therapy in the Million Women Study［J］.Lancet，2005，365（9470）：1543-1551.
［6］　KIM T H，KANG J W，KIM K H，KANG K W，SHIN M S，JUNG S Y，et al.Acupuncture for the treatment of dry eye：a multicenter randomised controlled trial with active comparison intervention （artificial teardrops）［J］.PLoS One，2012，7（5）：e36638.
［7］　Chinese Medical Association of Ophthalmology Branch of the Department of Keratology.Expert consensus of dry eye clinical diagnosis and treatment （2013）［J］.Chin J Ophthalmol，2013，49（1）：73-75.
中华医学会眼科学分会角膜病学组.干眼临床诊疗专家共识（2013）［J］.中华眼科杂志，2013，49（1）：73-75.
［8］　SPITZER R L，KROENKE K，WILLIAMS J B，LWE B.A brief measure for assessing generalized anxiety disorder：the GAD-7［J］.Arch Intern Med，2006，166（10）：1092-1097.
［9］　LIN J D，LIN L P，KUO M T，HSU S W，LEE T N，LAI C I，WU J L.Assessment of the 9-item Patient Health Questionnaire （PHQ-9） as a screen tool for symptoms of depression in community caregivers for adults with intellectual disabilities［J］.Res Dev Disabil，2014，35（1）：178-184.
［10］　SCHIFFMAN R M，CHRISTIANSON M D，JACOBSEN G，HIRSCH J D，REIS B L.Reliability and validity of the ocular surface disease index［J］.Arch Ophthalmol，2000，118（5）：615-621.
［11］　YANG L，YANG Z，YU H，SONG H.Acupuncture therapy is more effective than artificial tears for dry eye syndrome：evidence based on a meta-analysis［J］.Evid Based Complement Alternat Med，2015，2015：143858.
［12］　BHAVSAR A S，BHAVSAR S G，JAIN S M.A review on recent advances in dry eye：Pathogenesis and management［J］.Oman J Ophthalmol，2011，4（2）：50-53.
［13］　JEON J H，SHIN M S，LEE M S，JEONG S Y，KANG K W，KIM Y I，et al.Acupuncture reduces symptoms of dry eye syndrome：a preliminary observational study［J］.J Altern Complement Med，2010，16（12）：1291-1294.
［14］　ZHANG A，YAN G，SUN H，CHENG W，MENG X，LIU L，et al.Deciphering the biological effects of acupuncture treatment modulating multiple metabolism pathways［J］.Sci Rep，2016，6：19942.
［15］　WANG Y，JIANG H，MENG H，LI J，YANG X，ZHAO B，et al.Antidepressant mechanism research of acupuncture：Insights from a genome-wide transcriptome analysis of frontal cortex in rats with chronic restraint stress［J］.Evid Based Complement Alternat Med，2017，2017：1676808.
［16］　AQRAWI L A，GALTUNG H K，VESTAD B，OVSTEBO R，THIEDE B，RUSTHEN S，et al.Identification of potential saliva and tear biomarkers in primary Sjgren’s syndrome，utilising the extraction of extracellular vesicles and proteomics analysis［J］.Arthritis Res Ther，2017，19（1）：14.
［17］　SALL K，STEVENSON O D，MUNDORF T K，REIS B L.Two multicenter，randomized studies of the efficacy and safety of cyclosporin ophthalmic emulsion in moderate to severe dry eye［J］.Ophthalmology，2000，107（7）：631- 639.
［18］　YAZID S，GARDNER P J，CARVALHO L，CHU C J，FLOWER R J，SOLITO E，et al.Annexin-A1 restricts Th17cells and attenuates the severity of autoimmune disease［J］.J Autoimmun，2015，58：1-11.
［19］　SUN J，SHAO Z，YANG Y，WU D，ZHOU X，YUAN H.Annexin 1 protects against apoptosis induced by serum deprivation in transformed rat retinal ganglion cells［J］.Mol Biol Rep，2012，39（5）：5543-5551.
［20］　KUZNICKI J，KORDOWSKA J，PUZIANOWSKA M，WOZNIEWICZ B M.Calcyclin as a marker of human epithelial cells and fibroblasts［J］.Exp.Cell Res，1992，200（2）：425-430.
［21］　ZHOU L，BEUERMAN R W，CHAN C M，ZHAO S Z，LI X R，YANG H，et al.Identification of tear fluid biomarkers in dry eye syndrome using iTRAQ quantitative proteomics［J］.J Proteome Res，2009，8（11）：4889-4905.
［22］　ZHOU L，WEI R，ZHAO P，KOH S K，BEUERMAN R W，DING C.Proteomic analysis revealed the altered tear protein profile in a rabbit model of Sjgren’s syndrome-associated dry eye［J］.Proteomics，2013，13（16）：2469-2481.
［23］　SIM R，HIGUERA M，GARCA-RAMREZ M，CANALS F，GARCA-ARUM，HERNNDEZ C.Elevation of apolipoprotein A-I and apolipoprotein Hlevels in the vitreous fluid and overexpression in the retina of diabetic patients［J］.Arch Ophthalmol，2008，126（8）：1076-1081.
［24］　NYUNT A K，ISHIDA Y，YU Y，SHIMADA S.Topical apolipoprotein A-1 may have a beneficial effect on the corneal epithelium in a mouse model of dry eye：a pilot study［J］.Eye Contact Lens，2008，34（5）：287-292.
［25］　BAUSKAR A，MACK W J，MAURIS J，ARQUESO P，HEUR M，NAQEL B A，et al.Clusterin seals the ocular surface barrier in mouse dry eye［J］.PLoS One，2015，10（9）：e0138958.

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备注/Memo

备注/Memo:
国家自然科学基金面上项目（编号：81470648）
更新日期/Last Update: 2018-09-28