[1]丁小艳,董志章,王建洲,等.大气颗粒物PM2.5附着于角膜接触镜及其对角膜接触镜透氧性及折射率的影响[J].眼科新进展,2017,37(11):1001-1004.[doi:10.13389/j.cnki.rao.2017.0254]
　DING Xiao-Yan,DONG Zhi-Zhang,WANG Jian-Zhou,et al.Particulate matter 2.5 deposition on corneal contact lens and its effects on the oxygen permeability and refractive index of contact lenses[J].Recent Advances in Ophthalmology,2017,37(11):1001-1004.[doi:10.13389/j.cnki.rao.2017.0254]
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大气颗粒物PM2.5附着于角膜接触镜及其对角膜接触镜透氧性及折射率的影响/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年11期
页码:
1001-1004
栏目:
实验研究
出版日期:
2017-11-05

文章信息/Info

Title:
Particulate matter 2.5 deposition on corneal contact lens and its effects on the oxygen permeability and refractive index of contact lenses
作者:
丁小艳; 董志章; 王建洲; 李勇; 王燕慧; 许李彬; 王亚虹; 李娟
710018　陕西省西安市，西安市第三医院眼科（丁小艳，王建洲）；325200　浙江省温州市，温州医科大学附属第二医院眼科（董志章）；710004　陕西省西安市，西安市第四医院眼科（李勇，李娟）；712046　陕西省咸阳市，陕西省医疗器械质量监督检验院（王燕慧，许李彬）；710054　陕西省西安市，西安市环境监测站（王亚虹）
Author(s):
DING Xiao-Yan; DONG Zhi-Zhang; WANG Jian-Zhou; LI Yong; WANG Yan-Hui; XU Li-Bin; WANG Ya-Hong; LI Juan
Department of Ophthalmology，Xi ’an No.3 Hospital （DING Xiao-Yan，WANG Jian-Zhou），China；Department of Ophthalmology，the Second Affiliated Hospital of Wenzhou Medical University（DONG Zhi-Zhang），China；Department of Ophtalmology，Xi’an No.4 Hospital（LI Yong，LI Juan），China；Shaanxi Institute of Medical Device Quality Supervision and Inspeciton （WANG Yan-Hui，XU Li-Bin），China；Environmental Monitoring Station of Xi’an City（WANG Ya-Hong），China
关键词:
大气颗粒物; PM2.5; 角膜接触镜
Keywords:
atmospheric particulate matter; PM2.5; contact lens
分类号:
R772.2
DOI:
10.13389/j.cnki.rao.2017.0254
文献标志码:
A
摘要:
目的　观察大气颗粒物PM2.5在角膜接触镜的附着情况及其对角膜接触镜透氧性和折射率的影响。方法　采集大气颗粒物PM2.5，配制浓度为1 mg·mL-1的PM2.5混悬液，取硬性透气性角膜接触镜（rigid gas permeable contact lens，RGPCL）和软性角膜接触镜（soft contact lens，SCL）各18只，分为对照组、实验组和洗脱组，每组6只。实验组和洗脱组角膜接触镜浸入PM2.5混悬液中，室温振荡24 h。洗脱组经上述处理后转入无菌PBS中，室温振荡1 h。对照组镜片浸入无菌PBS中，室温振荡24 h。利用场发射扫描电子显微镜下观察PM2.5在各组角膜接触镜表面的附着情况。检测各组角膜接触镜的透氧性和折射率，记录结果，并进行统计分析。结果　经过PM2.5混悬液浸泡后，实验组RGPCL表面发现存在较多PM2.5附着，呈散在分布。实验组RGPCL表面大气颗粒物计数为每100 μm2（3.19±1.64）个。对照组电镜扫描未发现大气颗粒物沉着。经洗脱后，洗脱组RGPCL表面仍可见大量大气颗粒物附着，每100 μm2 （5.12±1.27）个。洗脱组与实验组差异有统计学意义（P＜0.05）。实验组SCL表面，大气颗粒物集中附着在SCL水凝胶孔隙边缘，而在孔隙中未见大气颗粒物附着，大气颗粒物细小，均匀；实验组SCL表面大气颗粒物计数为每100 μm2 （2.16±1.19）个。对照组SCL表面无大气颗粒物附着。经洗脱后，洗脱组SCL表面大气颗粒物计数为每100 μm2（0.56±0.39）个，洗脱组与实验组差异有统计学意义（P＜0.05）。对实验组RGPCL和SCL表面大气颗粒物附着数进行比较，RGPCL表面大气颗粒物附着数较多，差异有统计学意义（P＜0.05）。经过PM2.5混悬液浸泡后，实验组RGPCL的透氧性（100.00±3.17）与对照组（100.00±2.36）比较差异无统计学意义（P>0.05）。实验组SCL透氧性（42.00±2.57）与对照组（41.00±3.44）比较差异亦无统计学意义（P>0.05）。经过PM2.5混悬液浸泡24 h后，实验组RGPCL的折射率（1.415 6±0.000 4）与对照组（1.415 3±0.000 4）比较差异无统计学意义（P>0.05）。实验组SCL折射率（1.373 7±0.000 7）与对照组（1.373 2±0.000 1）比较差异亦无统计学意义（P>0.05）。结论　与SCL相比，PM2.5更易附着在RGPCL表面，且不易洗脱。在本研究条件下，PM2.5虽然附着于角膜接触镜，但短时间内并未对角膜接触镜的透氧性及折射率产生影响。
Abstract:
Objective　To observe the deposition of PM2.5 in the contact lens and its influence on the oxygen permeability and refractive index of the contact lens.Methods　Atmospheric PM2.5 was collected for preparation of 1 mg·mL-1 PM2.5 suspension.Then，18 rigid gas permeable contact lenses (RGPCL) and 18 soft contact lens lenses (SCL) were grouped in control，experimental and PBS-rinse group，with 6 RGPCL and 6 SCL in each group.RGPCL and SCL in the experimental and PBS-rinse groups were incubated in 1 mg·mL-1 PM2.5 solution for 24 h，followed by PBS-rinse group were further washed for 1 h in PBS，whereas the controls were incubated in PBS for 24 h.All of them were examined with field scanning electron microscopy.Meanwhile，oxygen permeability and refractive index of contact lens were measured using the polar graphic devices and refractometry respectively.All the obtained data were quantitatively and statistically analyzed.Results After treatment in PM2.5 solution，there was a large amount of PM2.5 deposition on the RGPCL surface in the experimental group，and the amount of PM2.5 was counted (3.19±1.64)·100 μm-2，while no atmospheric particulate matter was found in the control group；following the treatment of PBS，a lot of PM2.5 attached on the surface of RGPCL remained visible，and the number of particles was (5.12±1.27)·100 μm-2.And the difference was statistically significant between the control group and PBS-rinse group (P<0.05).In addition，and the amount of PM2.5 attached on the surface of SCL in the experimental group was (2.16±1.19)·100 μm-2，while there wasn’t any atmospheric particulate matter in the control group；and，after a wash of SCL in PBS，the amount of PM2.5 on SCL surface was (0.56±0.39)·100 μm-2 in the PBS-rinse group，indicating a statistically difference between the control group and PBS-rinse group (P<0.05).Moreover，the amount of PM2.5 attachment on SCL surface in the experimental group was significantly lower than that of the control group，and the difference was statistically significant (P<0.05).After PM2.5 solution treatment，oxygen permeability of RGPCL and SCL was 100.00±3.17 and 42.00±2.57 respectively in the experimental group，as well as 100.00±2.36 and 41.00±3.44 in the control group，with no statistically significant difference between the two groups (all P>0.05)；while refractive index of RGPCL and SCL was 1.415 6±0.000 4 and 1.373 7±0.000 7 respectively in the experimental group，as well as 1.415 3±0.000 4 and 1.373 7±0.000 1 in the control group，with no statistically significant difference between the two groups either (all P>0.05).Conclusion　PM2.5 can be attached to the surface of RGPCL and SCL，and PM2.5 is easier to adhere to RGPCL surface and is not easy to elute when compared with SCL，but PM2.5 deposition do not affect oxygen permeability and refractive index of contact lenses over a short span of time.

参考文献/References:

［1］　PAUlIN L，HANSEl N.Particulate air pollution and impaired lung function［J］.F1000Res，2016，5：201.
［2］　MENG X，ZHANG Y，YANG KQ，YANG YK，ZHOU XL.Potential harmful effects of PM2.5 on occurrence and progression of acute coronary syndrome：epidemiology，mechanisms，and prevention measures［J］.Int J Environ Res Public Health，2016，13（8）：E748.
［3］　JANG AS，JUN YJ，PARK MK.Effects of air pollutants on upper airway disease［J］.Curr Opin Allergy Clin Immunol，2016，16（1）：13-17.
［4］　JIA YY，WANG Q，LIU T.Toxicity research of PM2.5 compositions in vitro［J］.Int J Environ Res Public Health，2017，14（3）：E232.
［5］　FULLER CH，FEESER KR，SARNAT JA，O’NEIll MS.Air pollution，cardiovascular endpoints and susceptibility by stress and material resources：a systematic review of the evidence［J］.Environ Health，2017，16（1）：58.
［6］　LUENSMANN D，JONES L.Protein deposition on contact lenses：the past，the present，and the future［J］.Cont Lens Anterior Eye，2012，35（2）：53-64.
［7］　SUCHECKI JK，DONSHIK P，EHLERS WH.Contact lens complications［J］.Ophthalmol Clin North Am，2003，16（3）：471-484.
［8］　钟巨斌，林千文，谭建新，张星亮.PM2.5对哮喘急性期小鼠脾脏CD4+T淋巴细胞分化的影响［J］.中华实用儿科临床杂志，2015，30（16）：1269-1272.
ZHONG JB，LIN QW，TAN JX，ZHANG CL.Effects of PM2.5 on the differentiation of splenic CD4 + T lymphocyte of acute asthma mice［J］.Chin J Appl Clin Pediatr，2015，30（16）：1269-1272.
［9］　WILLCOX MD.Solutions for care of silicone hydrogel lenses［J］.Eye Contact Lens，2013，39（1）：24-28.
［10］　LEE SE，KIM SR.Influence of tear protein deposition on the oxygen permeability of soft contact lenses［J］.J Ophthalmol，2017，2017：5131764.
［11］　吴安花，谭钢，邵毅，李娟.环境污染与眼表疾病的关系研究进展［J］.山东医药，2017，57（7）：101-104.
WU AH，TAN G，SHAO Y，LI J.The relationship between environmental factors and ocular surface disease［J］.Shangdong Med J，2017，57（7）：101-104.
［12］　HAN JY，KANG B，EOM Y，KIM HM，SONG JS.Comparing the effects of particulate matter on the ocular surfaces of normal eyes and a dry eye rat model［J］.Cornea，2017，36（5）：605-610.
［13］　李娟，丁小艳，王亚虹，叶鳞泓，叶蕾，邵毅，等.PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响［J］.眼科新进展，2017，37（3）：201-204.
LI J，DING XY，WANG YH，YE LH，YE L，SHAO Y，et al.Effects of PM2.5 on tear film function and corneal epithelial structure in mice［J］.Rec Adv Ophthalmol，2017，37（3）：201-204.

相似文献/References:

[1]李娟,丁小艳,王亚虹,等.PM2.5对小鼠泪膜功能和角膜上皮组织结构的影响[J].眼科新进展,2017,37(3):201.[doi:10.13389/j.cnki.rao.2017.0052]
　LI Juan,DING Xiao-Yan,WANG Ya-Hong,et al.Effects of PM2.5 on tear film function and corneal epithelial structure in mice[J].Recent Advances in Ophthalmology,2017,37(11):201.[doi:10.13389/j.cnki.rao.2017.0052]

备注/Memo

备注/Memo:
国家自然科学基金资助（编号：81400424）；陕西省科学技术研究发展计划项目（编号：2014K11-03-07-04）；2017年陕西省创新人才推进计划项目——青年科技新星项目（编号：2017KJXX-87）
更新日期/Last Update: 2017-11-01