[1]陈红,文小凤,邓裕华,等.干眼患者眼表宏基因组研究[J].眼科新进展,2017,37(2):129-132.[doi:10.13389/j.cnki.rao.2017.0034]
　CHEN Hong,WEN Xiao-Feng,DENG Yu-Hua,et al.Study of ocular surface macro genome in dry eye patients[J].Recent Advances in Ophthalmology,2017,37(2):129-132.[doi:10.13389/j.cnki.rao.2017.0034]
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干眼患者眼表宏基因组研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
37卷
期数:
2017年2期
页码:
129-132
栏目:
应用研究
出版日期:
2017-02-05

文章信息/Info

Title:
Study of ocular surface macro genome in dry eye patients
作者:
陈红; 文小凤; 邓裕华; 魏来; 彭广华
100853　北京市，解放军总医院 眼科（陈红，彭广华）；510060　广东省广州市，中山大学中山眼科中心，眼科学国家重点实验室（文小凤，邓裕华，魏来）
Author(s):
CHEN Hong; WEN Xiao-Feng; DENG Yu-Hua; WEI Lai; PENG Guang-Hua
Department of Ophthalmology,General Hospital of Chinese PLA(CHEN Hong,PENG Guang-Hua),Beijing 100853,China;
关键词:
干眼; 眼表微生物组; 宏基因组测序; 抗性基因
Keywords:
dry eye; ocular surface microbiota; metagenomic shotgun sequencing; antibiotic resistance genes
分类号:
R777.34
DOI:
10.13389/j.cnki.rao.2017.0034
文献标志码:
A
摘要:
目的　通过比较干眼患者和健康受试者眼表宏基因组的异同探讨眼表微生物组的改变在干眼发病机制中的作用。方法　在解放军总医院眼科和中山大学中山眼科中心收集干眼患者20例及健康受试者90名。对所有受试者双眼进行结膜印迹细胞样本采集，提取核酸后进行宏基因组测序。通过计算Shannon指数比较两者微生物群落α多样性的差异；对两者微生物种群的相对丰度及代谢途径进行生物信息学分析。并对微生物中的抗生素抗性基因进行比对。结果　干眼患者眼表微生物的α多样性与健康受试者相比差异无统计学意义（P=0.13）。干眼患者眼表15种微生物的相对丰度较高，健康受试者10种微生物的相对丰度较高，且在代谢途径上存在着差异。干眼患者眼表微生物中的抗生素抗性基因明显多于健康受试者。结论　虽然干眼患者眼表微生物的多样性与健康受试者相似，但某些特定微生物种群的相对丰度及代谢途径存在特征性的改变，表明这些特定的微生物种群可能与干眼的发病机制有关。
Abstract:
Objective　To investigate the difference in ocular surface microbiota between dry eye patients and healthy subjects,and discuss the role of microbiota in dry eye.Methods　Twenty cases of dry eye patients and 90 cases of healthy subjects were collected in the PLA General Hospital and Zhongshan Ophthalmic Center.The samples of conjunctiva impression cytology were collected from all subjects,and then metagenomic shotgun sequencing was performed following the DNA extraction.The differences in alpha diversity and metabolic pathways of the ocular surface microbiota between dry eye patients and healthy subjects were evaluated.Results　There was no significant difference in alpha diversity of the microbial community between dry eye patients and healthy subjects (P=0.13).However,an increase of 15 species and a decrease of 10 species were detected on the ocular surface of dry eye patients.The enriched antibiotic resistance genes in dry eye patients were more than healthy subjects.Conclusion　Although the alpha diversity of the microbial community on ocular surface between dry eye patients and healthy subjects are not distinguishable,a significant difference could be found in relative abundance and metabolic pathways,suggest that these specific microbiome may be related to the pathogenesis and disease progression of dry eye.

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备注/Memo

备注/Memo:
国家自然科学基金面上项目（编号：31271400、31470065）；国家重点基础研究发展计划（编号：2013CB967001）
更新日期/Last Update: 2017-03-01