[1]陈凌燕,钟晖,张国明,等.基质细胞衍生因子对人视网膜微血管内皮细胞的作用[J].眼科新进展,2016,36(8):716-719.[doi:10.13389/j.cnki.rao.2016.0190]
　CHEN Ling-Yan,ZHONG Hui,ZHANG Guo-Ming,et al.Effects of SDF-1 0n proliferation and migration of human retinal capillary endothelial cells[J].Recent Advances in Ophthalmology,2016,36(8):716-719.[doi:10.13389/j.cnki.rao.2016.0190]
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基质细胞衍生因子对人视网膜微血管内皮细胞的作用
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
36卷
期数:
2016年8期
页码:
716-719
栏目:
实验研究
出版日期:
2016-08-05

文章信息/Info

Title:
Effects of SDF-1 0n proliferation and migration of human retinal capillary endothelial cells
作者:
陈凌燕; 钟晖; 张国明; 方旺; 陈仁典
518026　广东省深圳市，深圳市儿童医院眼科（陈凌燕，钟晖，方旺，陈仁典）；518040　广东省深圳市，深圳市眼科医院（张国明）
Author(s):
CHEN Ling-Yan;  ZHONG Hui;  ZHANG Guo-Ming;  FANG Wang;  CHEN Ren-Dian
Department of Ophthalmology, Shenzhen Children ’ s Hospital ( CHEN Ling-Yan, ZHONG; Hui, FANG Wang, CHEN Ren-Dian ) , Shenzhen 518026, Guangdong Province , China ; Shenzhen Eye Hospital ( ZHANG Guo-Ming ) , Shenzhen 518040 , Guangdong Province . China
关键词:
基质细胞衍生因子; 内皮细胞生长因子; 人视网膜微血管内皮细胞
Keywords:
stromal cell-derived factor-1 ;  vascular endothelial growth factor;  human retinal capillary endothelial cell
分类号:
Ｒ７７７
DOI:
10.13389/j.cnki.rao.2016.0190
文献标志码:
A
摘要:
目的　观察基质细胞衍生因子（ｓｔｒｏｍａｌｃｅｌｌ-ｄｅｒｉｖｅｄｆａｃｔｏｒ-１，ＳＤＦ-１）对体外培养的人视网膜微血管内皮细胞（ｈｕｍａｎｒｅｔｉｎａｌｃａｐｉｌｌａｒｙｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｓ，ＨＲＣＥＣｓ）增殖和迁移活性的影响。方法　体外培养ＨＲＣＥＣｓ，以ＭＴＴ法和Ｔｒａｎｓｗｅｌｌ实验分别检测ＳＤＦ-１和ＶＥＧＦ干预下细胞增殖、迁移活性的改变，二者的浓度分别为５ｎｇ·ｍＬ－１、５０ｎｇ·ｍＬ－１、１００ｎｇ·ｍＬ－１。联合５ｎｇ·ｍＬ－１ＶＥＧＦ和１００ｎｇ·ｍＬ－１ＳＤＦ-１观察二者的相互作用。结果　ＶＥＧＦ促细胞增殖呈浓度依赖型，５ｎｇ·ｍＬ－１、５０ｎｇ·ｍＬ－１和１００ｎｇ·ｍＬ－１组吸光度值分别为０．３０４±０．０３３、０．４１７±０．００１和０．４４７±０．０２５，均明显大于阴性对照组０．２０７±０．００３（均为Ｐ＜０．０５）；ＳＤＦ-１作用组吸光度值分别为０．２１±０．０３、０．２２±０．０５、０．２１±０．１１，与阴性对照组相比均无明显改变（均为Ｐ＞０．０５）。１００ｎｇ·ｍＬ－１ＳＤＦ-１联合５ｎｇ·ｍＬ－１ＶＥＧＦ共同作用时，细胞增殖活性（０．４７±０．０７）与阴性对照组相比明显增高（Ｐ＜０．０１）。ＨＲＣＥＣｓ迁移活性呈ＳＤＦ-１浓度依赖性，在５０ｎｇ·ｍＬ－１和１００ｎｇ·ｍＬ－１时，细胞迁移计数分别为４８０．０±３５．６和７００．０±７１．２，与阴性对照组相比均有显著性差异（均为Ｐ＜０．０１），联合５ｎｇ·ｍＬ－１ＶＥＧＦ促迁移活性明显增加。结论　ＳＤＦ-１可以促ＨＲＣＥＣｓ迁移，并提高ＶＥＧＦ促ＨＲＣＥＣｓ的增殖作用。
Abstract:
Objective To analyze the effects of stromal cell-derived factor-l (SDF-I) on proliferation and migration of human retinal capillary endothelial cells ( HRCECs) in vitro. Methods HRCECs were cultured in vitro. Cell proliferation was studied by MTT under different concentrations (5 ng . mL -1 ,50 ng . mL -l and 100 ng . mL-’) of SDF-I and VEGF, and Transwell was used to evaluate chemotaxis effect of SDF-I on HRCECs. The interactive effect of 100 ng . mL-l SDF-1 and 5 ng . mL -l VEGF was observed. Results On HRCECs ,VEGF induced a dose-dependent increase in cell proliferation.OD value of 5 ng . mL-’,50 ng . mL-1 and 100 ng . mL-l VEGF groups were 0. 304 + 0. 033 . 0. 417 + 0. 00 I and 0. 447 + 0. 025 , respectively , which were higher than the control group ( 0. 207 +0. 003) ( all P < 0. 05 ) . OD value of 5 ng . mL -1 ,50 ng . mL-] and 100 ng . mL-I SDF-I groups were 0. 21 +0. 03 .0. 22 +0. 05 ,0. 21 + 0. 11 , respectively , there was no statistical difference compared with the control group ( all P > 0. 05 ) . However . 100 ng . mL -1 SDF-I combined with 5 ng . mL -l VEGF promoted a significant increase in proliferation ( 0. 47 + 0. 07) compared with the control group (P < 0. 01). SDF-I led a dose-dependent increase in cell migration, which significantly increased in 50 ng . mL-1 and 100 ng . mL -l groups with cell count of 480. 0 + 35. 6 and 700. 0 + 71. 2 , respectively , there was sigruficant difference compared with the control group (P < 0. 01) ; And this effect could be amplified when combination with 5 ng . mL -1 VEGF. Conclusion SDF-I can promote the migration of HRCECs . and enhance the proliferative effect of VEGF.

备注/Memo

备注/Memo:
深圳市科技研发基金资助（编号：ＪＣＹＪ２０１３０４０１１５４７０９１６６、ＪＣＹＪ２０１３０４０１１１４１１１４６８）；深圳市科技计划项目（编号：２０１３０３０６５）
更新日期/Last Update: 2016-08-22