«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:

35卷

期数:

2015年11期

页码:

1036-1038

栏目:

实验研究

出版日期:

2015-11-05

文章信息/Info

Title:

Effects of LY294002 and octreotide on bFGF-induced proliferation of lens epithelial cells in rabbits

作者:

冯希敏; 张凤妍

453000　河南省新乡市，新乡市中心医院眼科

Author(s):

FENG Xi-Min ; ZHANG Feng-Yan

Department of Ophthalmology ,Xinxiang Central Hospital. Xinxiang 453000 , Henan Province . China

关键词:

LY294002; 奥曲肽; 晶状体上皮细胞; 后发性白内障

Keywords:

LY294002 ;  octreotide ; lens epithelial cells ;  posterior capsular opacification

DOI:

10.13389/j.cnki.rao.2015.0283

文献标志码:

A

摘要:

目的　探讨ＬＹ２９４００２联合奥曲肽对碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）诱导的兔晶状体上皮细胞（ｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌｌｓ，ＬＥＣｓ）增殖的影响。方法　兔眼ＬＥＣｓ经原代和传代培养后，共分为４组：空白对照组、增殖对照组、实验药物组、增殖药物组。空白对照组使用仅含无血清ＤＭＥＭ培养；增殖对照组使用加ｂＦＧＦ（１０ｍｇ·Ｌ－１）的无血清ＤＭＥＭ；实验药物组培养时在不加ｂＦＧＦ增殖的情况下分别单独应用１０－５ｍｏｌ·Ｌ－１ＬＹ２９４００２、单独应用１０－９ｍｏｌ·Ｌ－１奥曲肽和联合应用１０－５ｍｏｌ·Ｌ－１ＬＹ２９４００２与１０－９ｍｏｌ·Ｌ－１奥曲肽来培养；增殖药物组在加ｂＦＧＦ增殖的情况下分别单独应用１０－５ｍｏｌ·Ｌ－１ＬＹ２９４００２、单独应用１０－９ｍｏｌ·Ｌ－１奥曲肽和联合应用１０－５ｍｏｌ·Ｌ－１ＬＹ２９４００２和１０－９ｍｏｌ·Ｌ－１奥曲肽进行细胞培养，每组重复５次。各组分别培养４８ｈ。ＭＴＴ比色法测定吸光度（Ａ值）分析各组细胞的生长抑制率，流式细胞仪检测各周期细胞的百分率。结果　增殖对照组与空白对照组相比，吸光度Ａ值升高（Ｐ＜０．０５）；实验药物组中各组分别与空白对照组相比，吸光度Ａ值均有不同程度下降（均为Ｐ＜０．０５）；增殖药物组中各组分别与增殖对照组相比，吸光度Ａ值明显下降（均为Ｐ＜０．０５）；生长抑制率与吸光度Ａ值趋势相同。经流式细胞仪分析，实验药物组中联合用药组与两个单独用药组相比Ｇ０／Ｇ１期细胞比例增高，Ｓ期细胞比例降低（均为Ｐ＜０．０５）；增殖药物组中，联合用药组与两个单独用药组相比也出现Ｇ０／Ｇ１期细胞比例增高，Ｓ期细胞比例降低（均为Ｐ＜０．０５）。结论　ＬＹ２９４００２联合奥曲肽较单独用药对ＬＥＣｓ的增殖抑制作用更强。这为临床筛选药物防治后发性白内障提供依据。

Abstract:

Objective To investigate the proliferation inhibition effects of LY294002 and octreotide on rabbit lens epithelial cells ( LEC ) induced by basic fibroblast growth factor ( bFGF) . Methods LEC were isolated from rabbit, primarily cultured and passaged, divided into four groups: Blank control group, positive control group, drugs groups,positive drugs groups. LEC was cultured in free-serum DMEM as blank control group. bFGF( 10 mg . L ’l ) was used to induce the proliferation of LEC in positive control group. The drugs groups were grven only 10 -5 mol . L-l LY294002 , only 10 -9 mol . L -l octreotide and 10 -5 mol . L -l LY294002 + 10 -9 mol . L -l octreotide , rspectively. The positive drugs groups were used only 10 -5 mol . L -1 LY294002 , only 10 -9 mol . L-l octreotide and 10 -i mol . L-1 LY294002 + 10 -9 mol . L-l octreotide , the bFGF( 10 mg . L ’l ) was used to induce the proliferation of LEC.Then cultured for 48 hours. The absorbancy(A value ) was detected by methyl thiazolyl tetrazolium ( MTT ) for the assessment of inhibiting rate of LEC in different groups,and percentage of LEC in different cell cycles was tested and analyzed with flow cytometry. Results Compared with blank control group , the A value was elevated in positive control group ( P < 0. 05 ) . Compared with blank control group , the A value were obviously decreased to different extent in drugs groups( all P < 0. 05 ) . The more obvious descend in A values was seen in positive drugs groups compared with positive control groups ( all P < 0. 05 ) . In positive drugs group , compared with the octreotide + bFGF group and the LY294002 + bFGF group , the A values of the combined drug + bFGF group were all decreased( all P < 0. 05 ) . The inhibiting rate and the A value showed the same tendency. In drugs groups, compared with only drugs,the percentage of LEC of the combined drugs groups in GO/GI phase was increased( all P < 0. 05 ) .and that in S phase was decreased compared with only drugs groups ( all P < 0. 05 ) . Conclusion Compared with only drugs groups,combined drugs groups can more effectively inhibit the proliferation of LEC of rabbit. This result offers a basis for the clinical treatment of posterior capsular opacification.

常用功能

更新日期/Last Update: 2015-11-03